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Enzymatic properties of Staphylococcus aureus adenosine synthase (AdsA)

BMC Biochemistryvolume 12, Article number: 56 (2011) | Download Citation

Abstract

Background

Staphylococcus aureus is a human pathogen that produces extracellular adenosine to evade clearance by the host immune system, an activity attributed to the 5'-nucleotidase activity of adenosine synthase (AdsA). In mammals, conversion of adenosine triphosphate to adenosine is catalyzed in a two-step process: ecto-nucleoside triphosphate diphosphohydrolases (ecto-NTDPases) hydrolyze ATP and ADP to AMP, whereas 5'-nucleotidases hydrolyze AMP to adenosine. NTPDases harbor apyrase conserved regions (ACRs) that are critical for activity.

Results

NTPDase ACR motifs are absent in AdsA, yet we report here that recombinant AdsA hydrolyzes ADP and ATP in addition to AMP. Competition assays suggest that hydrolysis occurs following binding of all three substrates at a unique site. Alanine substitution of two amino acids, aspartic acid 127 and histidine 196 within the 5'-nucleotidase signature sequence, leads to reduced AMP or ADP hydrolysis but does not affect the binding of these substrates.

Conclusion

Collectively, these results provide insight into the unique ability of AdsA to produce adenosine through the consecutive hydrolysis of ATP, ADP and AMP, thereby endowing S. aureus with the ability to modulate host immune responses.

Background

Staphylococcus aureus is a Gram-positive pathogen and the leading cause of bloodstream, lower respiratory tract, skin and soft tissue infections [1]. S. aureus produces numerous virulence factors that contribute to its ability to cause disease [2–4]. These include several toxins that are known for their detrimental effects on host cells [5, 6], in particular cells of the immune system [7, 8]. Staphylococci can infect a broad range of tissues and organs resulting in excessive tissue damage [9]. This observation is highlighted by the appearance of large populations of necrotic cells surrounding staphylococcal communities within organ abscesses isolated from infected mice [10]. Cellular damage caused by bacterial triggers the release of otherwise sequestered intracellular components such as heat shock proteins (HSPs) [11], S100 proteins [12], nucleosomes [13], N-formylated mitochondrial peptides [14] and purines (ATP and ADP) [15, 16] all of which are known to potently stimulate inflammation. Excessive inflammation can be detrimental to the host due to the prolonged presence of activated immune cells as well as the leakage of proteases and other noxious agents that damage surrounding tissues. A delicate balance of pro- and anti-inflammatory mediators is critical to prevent extensive inflammation.

Extracellular nucleotides (i.e. adenosine tri-, di- and monophosphates and adenosine), which signal through purinergic cell surface receptors have recently been shown to serve as mediators of inflammation. For example, stimulation of purinergic PY receptors by ATP and ADP results in pro-inflammatory responses while stimulation of PX adenosine receptors leads to an anti-inflammatory response [17–22]. In addition, nucleotide metabolizing enzymes that hydrolyze adenosine tri- and di-phosphates (ATP and ADP) or adenosine monophosphates (AMP), termed ecto-nucleoside triphosphate diphosphohydrolases (ecto-NTPDases) or 5'-nucleotidases respectively, regulate purinergic signaling by controlling the level of extracellular nucleotides. NTPDases hydrolyze nucleoside tri- and/or diphosphates, but not monophosphates [23–25]. Eight members of the NTPDase family have been identified in mammals, all of which are characterized by five highly conserved sequence motifs known as "apyrase conserved regions" (ACR), which range from 4-13 residues in length [26]. CD39 (NTDPase 1) was the first member identified for this family of enzymes. It is expressed on activated B cells and regulatory T (Treg) cells. Hydrolysis of 5'-AMP is carried out by a second class of enzymes. CD73 is the best characterized of the 5'-nucleotidases; CD73 hydrolyzes 5'-AMP specifically and shows no activity towards 2'- and 3'-monophosphates [27]. This ecto-enzyme is expressed in different tissues, with abundant expression in the colon, kidney, liver, heart, lung and on specific cells of the immune system [27, 28]. CD73 and CD39 are co-expressed on the surfaces of CD+4/CD+25/Foxp3+ Treg cells and catalyze the enzymatic conversion of ATP/ADP-derived AMP into the anti-inflammatory mediator adenosine, subsequently leading to inhibition of T cell proliferation and secretion of cytokines [29, 30].

We recently reported that S. aureus AdsA, a cell wall anchored protein, is a 5'-nucleotidase that catalyzes the conversion of AMP to adenosine. The nucleotidase activity of AdsA is critical for S. aureus survival in blood and adsA mutants are impaired in their ability to induce abscess formation during infection [31]. Thus, we surmise that S. aureus uses AdsA to increase the concentration of adenosine concentrations within the host and take advantage of adenosine's immunosuppressive properties to escape immune clearance. Since staphylococci are surrounded by large populations of dead or dying host cells within deep tissue abscesses [10], it can be assumed that there is an abundance of extracellular nucleotides released from damaged tissues. The importance of extracellular nucleotide signaling in mediating pathogen clearance led us to further investigate AdsA's nucleotide metabolizing capacity. Although analyses of the amino acid sequence of AdsA did not reveal conserved ACR motifs indicative of NTPDases, a recombinant AdsA was able to efficiently hydrolyze both ATP and ADP in vitro. We further characterized the enzyme kinetics of AdsA hydrolysis of ATP, ADP and AMP and also identified amino acid residues critical for AdsA's hydrolase activity.

Methods

Purification of recombinant AdsA

Recombinant GST-tagged AdsA (rAdsA) was expressed using pVT1 in Escherichia coli BL21 (DE3) and purified using glutathione S-transferase affinity chromatography as described previously [31]. The N-terminal GST tag was cleaved with thrombin and thrombin removed by incubation with benzamadine sepharose beads per manufacturer's conditions (GE Healthcare).

Assays for enzymatic activity of AdsA

Hydrolysis of ATP, ADP and AMP (Sigma-Aldrich) was carried out in 50 mM Tris-HCl buffer pH 7.5, in the presence of 1 mM nucleotide and 0.5 mM MnCl2. rAdsA was added to the reaction at 0.15 μg/μl and the reaction was incubated at 37°C for 15 min. Inorganic phosphate release was detected by addition of malachite green dye reagent [32] (1.1% w/v ammonium molybdate, 0.04% w/v malachite green hydrochloride) and 3.4% citric acid and concentration was calculated from a known concentration range of phosphate standards. Similar conditions were used to determine the pH optima of rAdsA for AMP and ADP. Inorganic phosphate release was also recorded using malachite green dye reagent to assess hydrolysis of non-adenine based nucleotides. To determine the divalent cation preference of rAdsA, ADP hydrolysis (1 mM) was assayed in 50 mM Tris-HCL buffer pH 7.5, containing either 0-5 mM MgCl2 or MnCl2, or 0-2.5 mM ZnCl2, or CuSO4. Thin layer chromatography was performed as previously described [31]. Purified rAdsA (2 μM) was incubated in a 15 μl reaction volume with increasing amounts of [14C]AMP (Moravek biochemicals) in 50 mM Tris-HCL buffer, pH 7.5 containing 0.5 M sucrose and 0.5 mM MnCl2. Samples were incubated for 15 minutes and then spotted onto silica plates, followed by separation by TLC using a 75:25 isopropanol/double distilled H2O-0.2 M ammonia bicarbonate solvent. 75 μM cold nucleotide (AMP or ADP) was used for competitive inhibition experiments.

Site-directed mutagenesis of rAdsA

The following primers were used for PCR amplification reactions:

D127F (5'-ACAACACATAAAATATTACA TACAAATGCTATCCATGGCCGACTAGC-3'), D127R (5'-GCTAGTCGGCCATGGATA GCATTTGTATGTAATATTTTATGTGTTGT-3'), H129F (5'-ACACATAAAATATTACATACAAATGATATCGCTGGCCGACTAGCCGAAG A-3'), H129R (5'-TCTTCGGCTAGTCGGCCAGCGATATCATTTGTATGTAATATTTTAT GTGT-3'), H196F (5'-GATG CTATGGCAGTCGGTAACGCTGAATTTGACTTTGGATAC-3'), H196R (5'-GTATCCA AAGTCAAATTCAGCGTTACCGACTGCCATAGCATC-3'), D199F (5'-GTCGGTAAC CATGAATTTGCCTTTGGATACGATCAGTTG-3'), D199R (5'-CAACTGATCGTATC CAAAGGCAAATTCATGGTTACCGAC-3'). Site-directed mutagenesis was performed using AccuPrime pfx DNA polymerase (Invitrogen) using pVT1 as a template for replacement of Asp127 to Ala (D127A), His129 to Ala (H129A), His196 to Ala (H196A), and Asp199 to Ala (D199A). All plasmids were transformed in E. coli BL21 (DE3) to produce recombinant variants. All mutations were confirmed by nucleotide sequencing of plasmid DNA.

Assessment of nucleotide binding to rAdsA

Binding of AMP and ADP to rAdsA was carried out as described [33]. Briefly, rAdsA (10 μM) was incubated with 9 μCi [14C]AMP or 9 μCi [14C]ADP for 15 min on ice in 50 mM Tris-HCl buffer, pH 7.5 containing 0.5 M MnCl2. Samples were adsorbed to a nitrocellulose membrane using a vacuum manifold and washed twice with 10 ml buffer. Radioactivity retained on the membranes was measured by scintillation counting.

Circular dichroism

rAdsA was dialyzed against 8 mM NaH2PO4, 1.5 mM Na2HPO4 buffer. Purified protein (100 μg/ml) was subjected to circular dichroïsm using an AVIV 202 CD spectrometer at room temperature.

Results

AdsA hydrolyzes adenosine nucleoside tri- and di-phosphates

We have previously shown that S. aureus AdsA hydrolyzes AMP to produce adenosine [31]. However, extracellular ATP and ADP stimulate inflammation as well. We wondered whether AdsA might also hydrolyze these molecules. Hydrolysis was assessed following incubation with rAdsA for 15 min by measuring the release of inorganic phosphate using the malachite green colorimetric assay. rAdsA hydrolyzed ATP and ADP efficiently. In fact, the enzyme released 1.5 fold more phosphate from ATP and ADP after 15 min incubation than from AMP (Figure 1A). In addition, rAdsA hydrolyzed both guanosine derivatives GTP and GDP, albeit at reduced efficiency compared to ATP and ADP. In comparison, the inosine nucleosides ITP and IDP were poor substrates for the enzyme (Figure 1B, C). Cytosine nucleosides were not hydrolyzed by rAdsA (Figure 1B, C).

Figure 1
figure1

AdsA efficiently hydrolyzes adenosine nucleoside tri- and diphosphates. The ability of rAdsA to hydrolyze nucleoside tri- or diphosphates was evaluated by assessing inorganic phosphate released after incubation of rAdsA with the indicated substrates for 15 minutes. Percent activity is expressed as the amount of inorganic phosphate released relative to ATP (A and C, 914 ± 70 nmol pi) and ADP (B, 840 ± 55 nmol pi) within each experiment. The results are the average of 3 independent analyses conducted in duplicate and the error bars represent the SEM.

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In mammals, nucleotide di- and tri-phosphate hydrolysis is primarily attributed to NTPDases whereas 5'-nucleotidases display specificity toward nucleotide mono-phosphate substrates. NTPDases encompass five conserved ACR motifs that form the active site of these enzymes. Such ACR motifs indicative of NTPDases cannot be found in the primary sequence of AdsA. NTPDases are rarely found in prokaryotes, however bacterial 5'-nucleotidases from Escherichia coli and Vibrio costicola have been shown to possess the capacity to hydrolyze ATP molecules [34, 35]. Thus, our results suggest that the bacterial 5'-nucleotidase AdsA utilizes a distinctive mechanism for the hydrolysis of ADP/ATP that has been shown to occur in mammalian NTPDases.

Kinetic activity of AdsA

Substrate preference for nucleoside di- and tri-phosphates varies among members of the ecto-NTPDase family of enzymes. For example, CD39/NTPDase1 hydrolyzes both ATP and ADP with similar efficiency, whereas NTPDase2 preferentially exhibits ATPase activity [26]. We examined and compared the relative rate of ATP, ADP and AMP hydrolysis by rAdsA at pH 7.5 in the presence of 0.5 mM MnCl2 (Figure 2A). The rate of inorganic phosphate released from all three nucleotides was linear over the first 20 min of incubation (with linear regression using GraphPad Prism5 software yielding r2 value of 0.90, 0.98 and 0.96 for AMP, ADP and ATP hydrolysis, respectively). The initial rate of reaction varied with the concentration of substrate (AMP or ADP) and followed the Michaelis-Menten kinetic model of a single-substrate reaction (Figure 2B). GraphPad Prism5 software was used to perform nonlinear regression analysis and a V max value 713 μmol Pi/min/mg for ADP hydrolysis compared to 391 μmol Pi/min/mg for AMP hydrolysis together with a calculated K m value of 0.93 mM for AMP and 0.51 mM for ADP. The total release of inorganic phosphate from 1 mM ADP was approximately 1.8 times higher than from 1 mM AMP after 10 minutes (Figure 2B). ADP contains two phosphate groups to a single phosphate group in AMP suggesting similar hydrolysis rates for both the α- and β-phosphate groups. However, only a slight increase in inorganic phosphate was observed from 1 mM ATP relative to 1 mM ADP over the same time period, suggesting that AdsA may preferentially hydrolyze the α- and β-phosphate groups of AMP and ADP compared to the γ-phosphate in ATP. Together these results confirm that AdsA is capable of hydrolyzing ATP and ADP in addition to its known AMP substrate.

Figure 2
figure2

Kinetic activity of AdsA. (A) hydrolysis of 1 mM AMP, 1 mM ADP and 1 mM ATP by 0.15 μg/μl rAdsA, measured as nmol Pi released over time. The results are expressed as nmol Pi released over time and the average of two independent analyses conducted in triplicate. Error bars represent the standard error of the mean. (B) Michaelis-Menten plots of enzyme velocity demonstrating the effect of varying AMP (upper panel) or ADP (lower panel) concentration as shown. Curve fitting using nonlinear regression was performed using GraphPad Prism5 software. The results are expressed as average μmol Pi released/min/mg protein and error bars represent the standard error of the mean. Data is representative of two independent analyses conducted in triplicate.

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Effect of pH and metal cations on AdsA activity

Increased concentrations of adenine nucleotides in the extracellular milieu may contribute to the drop in pH observed during severe inflammation and hypoxia. This prompted us to evaluate the effect of pH on both ADP and AMP hydrolysis over a pH range of 4-10. AdsA displayed optimal activity at pH 7.0 when AMP and ADP served as substrates. However, substantial ADPase activity was observed as low as pH 4.0 (Figure 3A, B). This finding suggests that the enzyme remains active in various acidic environments that bacteria encounter during host invasion.

Figure 3
figure3

AdsA is highly active at a near neutral pH and is dependent on metal cations. (A and B) Effect of pH on activity of rAdsA. Relative hydrolysis of 1 mm AMP (A) or 1 mm ADP (B) in buffer containing 50 mm Tris-HCl and 0.5 mM MnCl2 over the indicated pH range is shown. The results are expressed as the percentages of activity and are the average of three independent analyses. (C-D), Shown is the relative ADPase activity of AdsA in the presence of (C) MnCl2, (D) MgCl2, (E) ZnCl2, and (D) CuCl2. The results are expressed as relative activity of the highest value in each experiment. Data is the average of two independent analyses conducted in triplicate.

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All mammalian surface-located NTPDases are inactive in the absence of Mg2+ or Ca2+ cations [26]. In contrast, the majority of parasitic enzymes are stimulated by Mg2+ or Ca2+[36–38] and Zn2+. Hydrolysis of ATP and ADP by other bacterial 5'-nucleotidases require Mg2+ or Mn2+ and these enzymes are inhibited by Zn2+[34]. We have previously shown that optimum hydrolysis of AMP by staphylococcal AdsA is stimulated by Mg2+ and Mn2+ and inhibited by Zn2+ and Cu2+[31]. Here, we examine how divalent cations modulate the ADPase activity of rAdsA. Similar to AMP hydrolysis, we observe that optimal ADPase activity occurred in the presence of Mn2+ and Mg2+ specifically at 0.5 mM MnCl2 and MgCl2 (Figure 3C, D). The presence of Zn2+ and Cu2+ inhibited hydrolysis of ADP. The 50% Inhibitory Concentration (IC50) for Zn2+ and Cu2+ were calculated using non-linear regression as 159 μM and 512 μM, respectively (Figure 3E, F). These results suggest that hydrolysis of AMP and ADP may require similar catalytic reactions and active site residues.

Inhibition of AdsA 5'-nucleotidase activity by ADP

The crystal structure of S. aureus AdsA has not been determined and the molecular interactions between AdsA and its substrates are unknown. To examine AdsA's substrate interactions further, we asked whether ADP is a competitive inhibitor of rAdsA's 5'-nucleotidase activity. rAdsA was incubated with [14C]AMP and increasing concentrations of cold non-radiolabeled AMP or ADP and production of [14C]adenosine was measured by separation of substrate and product using thin layer chromatography (TLC) and recording of radioactive counts. (Figure 4A) and the data were analyzed using the non-linear curve fitting of the GraphPad Prism software to determine K m values (Figure 4B). Co-incubation of either cold AMP or ADP with [14C]AMP similarly inhibited the hydrolysis of [14C]AMP, as the K m value of [14C]AMP in the presence of either AMP or ADP was reduced to similar levels. Furthermore, Lineweaver-Burk analyses (Figure 4C) clearly show that cold AMP and ADP function as similar competitive inhibitors of the reaction, suggesting that ADP and AMP may compete for the same AdsA substrate binding site.

Figure 4
figure4

Inhibition of AdsA 5'-nucleotidase activity by ADP. 2 μM rAdsA was incubated with 0-500 μM [14C]AMP in the presence of 75 μM cold AMP or ADP as indicated. (A) Radioactive signals for [14C]AMP and [14C]Ado after TLC were captured by a phosphorimager. Data is representative of two independent analyses. (B) Curve fitting analysis of data from (A) using nonlinear regression was performed using GraphPad Prism5 software. The results are expressed as nmols [14C]adenosine produced/min/mg rAdsA and are the averages of two experiments performed in duplicate. (C) Lineweaver-Burk plot of data in (B).

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Residues in the 5'-nucleotidase signature sequence that contribute to AdsA activity

Extensive site-directed mutagenesis studies have been carried out with both CD39/NTPDase1 and NTPDase3 [39–42], as well as bacterial 5'-nucleotidases [27, 43, 44] to reveal the importance of conserved residues to catalytic activities. The structure-function analysis of E. coli 5'-nucleotidase (UDP-sugar hydrolase) identified residues within its first nucleotidase signature sequence that are implicated in binding divalent metal cations. This analysis also revealed that the enzyme's catalytic Asp-His dyad is located in the second nucleotidase signature sequence. Similar amino acid residues and signature sequences can be identified in the primary sequence of staphylococcal AdsA. Specifically, Asp127 and His129 are located in the first signature sequence, ILHTnD127iH129GrL, whereas His196 and Asp199 are located in the second signature sequence, YdamaVGNH196EFD199. To examine the contribution of these four amino acids to AdsA catalysis, we individually substituted Asp127, His129, His196 and Asp199 for alanine. Each variant rAdsA was purified and its catalytic activity and substrate specificity was compared to that of the wild-type enzyme.

The release of inorganic phosphate was monitored when using either AMP or ADP as substrates. Replacing His129 or Asp199 with alanine did not affect the ability of the enzyme to hydrolyze AMP or ADP. However, substitution of Asp127 to Ala reduced ADP hydrolysis to 30% ± 4% of wild type levels but did not affect the ability of rAdsA to hydrolyze AMP. Conversely, subsitution of His196 to Ala led to a moderate reduction of AMP hydrolysis (65% ± 3% of wild-type activity) but significantly affected the ADPase activity of the enzyme (23% ± 5% of wild-type activity) (Figure 5A,B). Next, we asked whether reduced AMP or ADP hydrolysis by the variant proteins (specifically Asp127Ala, His196Ala and Asp199Ala) could be explained by a loss of affinity for the substrates. Wild-type and variants of rAdsA were incubated with either [14C]AMP or [14C]ADP at 4°C degrees for 10 min and adsorbed onto a nitrocellulose membrane. Unbound [14C] labeled nucleotide was removed by extensive washes of the membranes and measurements of radioactivity counts were recorded as a direct assessment of nucleotide binding to the enzymes. All three mutants (Asp127Ala, His196Ala and Asp199Ala) bound [14C]AMP or [14C]ADP with affinities similar to the wild type rAdsA enzyme (Figure 5C,D). Furthermore, we recorded circular dichroism (CD) spectra and confirmed that none of the substitutions altered the secondary structure of rAdsA (Figure 5E). Together, these data identify rAdsA residues implicated in metal ion binding as well as its Asp-His catalytic dyad, two AdsA features that are shared with other 5'-nucleotidases.

Figure 5
figure5

Amino acid substitutions of conserved residues present in the 5'-nucleotidase signature sequences affects the level of AdsA activity. (A and B) hydrolysis of 1 mm AMP (A) or 1 mM ADP (B) by 0.15 μg/μl rAdsA measured as nmol Pi released over time. (C and D) Binding of AMP or ADP to rAdsA protein. Protein in mixtures of rAdsA and [14C]AMP (C) or [14C]ADP (D) were adsorbed onto nitrocellulose filters and the amount of radioactivity retained on the filter was determined. The results are expressed as radioactive counts per minute (cpm). Data is representative of two independent analyses conducted in triplicate and error bars represent the SEM. (E) CD spectra of WT rAdsA, rAdsAD127A and rAdsAH196A. (F) SDS-PAGE analyses of 5 μg protein as quantified by a BCA protein assay. Lanes 1-5 correspond to WT, D127A, H129A, H196A and D199A respectively.

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Discussion

We have previously shown that AdsA secreted by S. aureus hydrolyzes AMP to produce adenosine, which enhances the ability of S. aureus to evade immune clearance [31]. In this study, we defined the enzymatic properties of rAdsA and demonstrated that in addition to exhibiting 5'-nucleotidase activity, rAdsA also exhibits NTPDase activity. This was exemplified by rAdsA's ability to hydrolyze ADP and ATP and to a lower extent GDP and GTP, with its ADPase activity retained even under acidic conditions. In contrast, the enzyme was not able to utilize CDP or CTP.

In mammals, the conversion of ATP to adenosine requires the sequential activity of ecto-NTPDases and 5'-nucleotidases. The substrate specificities of the two types of enzyme are quite specific as CD39 (NTPDase1) cleaves ATP and ADP but not AMP [26] and likewise CD73 (5'-nucleotidase) cleaves AMP but not ATP and ADP. Substrate specificity is thought to result from structural differences between the binding pockets of NTPDases and 5'-nucleotidases. Active site residues lying within the NTPDase ACR motifs are shown to be situated in close proximity of the γ- and β-phosphate groups of ATP whereas the α-phosphate of an AMP molecule would be further buried and inaccessible. We identified amino acid residues Asp127 and His196 within the conserved 5'-nucleotidase signature sequences as being critical for ADP hydrolysis. Furthermore, results from the competitive inhibition experiments with cold nucleotide substrates displayed in Figure 4 imply that AdsA binds AMP and ADP at a single site. Together these observations suggest that the two 5'-nucleotidase signature sequences of AdsA lie within close proximity of the nucleotide binding pocket in a unique spacial orientation that allows for the removal of both the β- and α-phosphates. Comparison of crystal structures from AdsA bound to AMP or ADP substrates is needed to further our understanding of AdsA's unique enzymatic activity; this is currently being pursued in the laboratory.

The ability to produce adenosine from multiple substrates provides a clear advantage for S. aureus in the fight against the host's immune system. Initiation of staphylococcal infections usually involve bacterial invasion of the skin or blood stream via trauma, surgical wounds, or medical devices [45] and much is known about the mechanisms that S. aureus uses to combat the initial innate immune defense in the blood. Advanced S. aureus infection leads to dissemination of staphylococci into various tissues and formation of abscesses in organs. However, the molecular mechanisms of abscess formation during staphylococcal infections are not clearly understood but likely involve both pathogen and host response factors [46]. The architecture of kidney abscesses observed in cross sections of kidneys collected from mice 5 days after staphylococcal infection shows a central staphylococci community surrounded by several distinct layers of infiltrating immune cells [10]. Closer examination of the abscesses in the histological images reveals a population of necrotic immune cells directly surrounding foci of bacteria, which likely encompasses a localized environment rich in cellular debris. As high concentrations of nucleotides are likely available as substrates for AdsA, S. aureus may be able to increase the abundance of adenosine to local concentrations that are even higher than those observed in blood [31]. In turn, the accumulation of adenosine may diminish the bactericidal attributes of infiltrating immune cells or alter the spectrum of immune cells that arrive at the abscess lesions. Furthermore, it has been shown that inflammatory microenvironments are significantly more acidic due to hypoxia and high levels of adenosine. AdsA's ADPase activity is retained at low pH (Figure 3B), an attribute that may be important in pathogenesis under these conditions. Consistent with these observations, we have shown that kidneys isolated from mice infected with wild-type S. aureus harbored higher number of abscesses as compared to mice infected with isogenic adsA variants [31].

S. aureus is known to survive within the host for prolonged periods of time, however the mechanisms involved in such a lifestyle are not clearly known [reviewed in [47, 48]]. The conversion of GTP to GDP inside cells is critical to intracellular signaling events [49] and the ability of staphylococci to hydrolyze GTP substrates may play a role in host intracellular survival, an area of investigation that we believe warrants further examination.

Conclusions

We show that in addition to its 5'-nucleotidase function, AdsA is functionally similar to NTPDase enzymes, owing to its ability to hydrolyze both ATP and ADP substrates. AdsA does not harbor any NTPDase ACR motifs and suggests that bacterial 5'-nucleotidases such as AdsA may harbor a unique active site. Comparative structural analyses between AdsA and NTPDases may enable the future design of inhibitors that block not only S. aureus AdsA but perhaps even the AdsA homologs from other bacterial pathogens [31].

Abbreviations

AdsA:

adenosine synthase A

NTPDase:

nucleoside triphosphate diphosphorylase

ATP:

adenosine triphosphate

ADP:

adenosine diphosphate

AMP:

adenosine monophosphate

Ado:

adenosine

ACR:

apyrase conserved regions

CD:

cluster of differention

HSP:

heat shock protein

TLC:

thin layer chromatography

PCR:

polymerase chain reaction

CD:

circular dichroism

GTP:

guanosine triphosphate

GDP:

guanosine diphosphate

ITP:

inosine triphosphate

IDP:

inosine diphosphate.

References

  1. 1.

    Diekema DJ, Pfaller MA, Schmitz FJ, Smayevsky J, Bell J, Jones RN, Beach M: Survey of infections due to Staphylococcus species: frequency of occurrence and antimicrobial susceptibility of isolates collected in the United States, Canada, Latin America, Europe, and the Western Pacific region for the SENTRY Antimicrobial Surveillance Program, 1997-1999. Clin Infect Dis. 2001, 32: S114-132. 10.1086/320184.

  2. 2.

    Foster TJ, Hook M: Surface protein adhesins of Staphylococcus aureus. Trends Microbiol. 1998, 6 (12): 484-488. 10.1016/S0966-842X(98)01400-0.

  3. 3.

    Jin T, Bokarewa M, Foster T, Mitchell J, Higgins J, Tarkowski A: Staphylococcus aureus resists human defensins by production of staphylokinase, a novel bacterial evasion mechanism. J Immunol. 2004, 172 (2): 1169-1176.

  4. 4.

    Foster TJ: Immune evasion by staphylococci. Nat Rev Microbiol. 2005, 3 (12): 948-958. 10.1038/nrmicro1289.

  5. 5.

    Bubeck Wardenburg J, Patel RJ, Schneewind O: Surface proteins and exotoxins are required for the pathogenesis of Staphylococcus aureus pneumonia. Infect Immun. 2007, 75 (2): 1040-1044. 10.1128/IAI.01313-06.

  6. 6.

    Bubeck Wardenburg J, Bae T, Otto M, Deleo FR, Schneewind O: Poring over pores: alpha-hemolysin and Panton-Valentine leukocidin in Staphylococcus aureus pneumonia. Nat Med. 2007, 13 (12): 1405-1406. 10.1038/nm1207-1405.

  7. 7.

    Dinges MM, Orwin PM, Schlievert PM: Exotoxins of Staphylococcus aureus. Clin Microbiol Rev. 2000, 13 (1): 16-34. 10.1128/CMR.13.1.16-34.2000. table of contents

  8. 8.

    Wang R, Braughton KR, Kretschmer D, Bach TH, Queck SY, Li M, Kennedy AD, Dorward DW, Klebanoff SJ, Peschel A: Identification of novel cytolytic peptides as key virulence determinants for community-associated MRSA. Nat Med. 2007, 13 (12): 1510-1514. 10.1038/nm1656.

  9. 9.

    Lowy FD: Staphylococcus aureus infections. N Engl J Med. 1998, 339 (8): 520-532. 10.1056/NEJM199808203390806.

  10. 10.

    Cheng AG, Kim HK, Burts ML, Krausz T, Schneewind O, Missiakas DM: Genetic requirements for Staphylococcus aureus abscess formation and persistence in host tissues. Faseb J. 2009, 23 (10): 3393-3404. 10.1096/fj.09-135467.

  11. 11.

    Panjwani NN, Popova L, Srivastava PK: Heat shock proteins gp96 and hsp70 activate the release of nitric oxide by APCs. J Immunol. 2002, 168 (6): 2997-3003.

  12. 12.

    Hofmann MA, Drury S, Fu C, Qu W, Taguchi A, Lu Y, Avila C, Kambham N, Bierhaus A, Nawroth P: RAGE mediates a novel proinflammatory axis: a central cell surface receptor for S100/calgranulin polypeptides. Cell. 1999, 97 (7): 889-901. 10.1016/S0092-8674(00)80801-6.

  13. 13.

    Hefeneider SH, Cornell KA, Brown LE, Bakke AC, McCoy SL, Bennett RM: Nucleosomes and DNA bind to specific cell-surface molecules on murine cells and induce cytokine production. Clin Immunol Immunopathol. 1992, 63 (3): 245-251. 10.1016/0090-1229(92)90229-H.

  14. 14.

    Carp H: Mitochondrial N-formylmethionyl proteins as chemoattractants for neutrophils. J Exp Med. 1982, 155 (1): 264-275. 10.1084/jem.155.1.264.

  15. 15.

    Cronstein BN, Daguma L, Nichols D, Hutchison AJ, Williams M: The adenosine/neutrophil paradox resolved: human neutrophils possess both A1 and A2 receptors that promote chemotaxis and inhibit O2 generation, respectively. J Clin Invest. 1990, 85 (4): 1150-1157. 10.1172/JCI114547.

  16. 16.

    Poelstra K, Heynen ER, Baller JF, Hardonk MJ, Bakker WW: Modulation of anti-Thy1 nephritis in the rat by adenine nucleotides. Evidence for an anti-inflammatory role for nucleotidases. Lab Invest. 1992, 66 (5): 555-563.

  17. 17.

    Cronstein BN, Kramer SB, Weissmann G, Hirschhorn R: Adenosine: a physiological modulator of superoxide anion generation by human neutrophils. J Exp Med. 1983, 158 (4): 1160-1177. 10.1084/jem.158.4.1160.

  18. 18.

    Hasko G, Cronstein BN: Adenosine: an endogenous regulator of innate immunity. Trends Immunol. 2004, 25 (1): 33-39. 10.1016/j.it.2003.11.003.

  19. 19.

    Hasko G, Pacher P: A2A receptors in inflammation and injury: lessons learned from transgenic animals. J Leukoc Biol. 2008, 83 (3): 447-455.

  20. 20.

    Honda S, Sasaki Y, Ohsawa K, Imai Y, Nakamura Y, Inoue K, Kohsaka S: Extracellular ATP or ADP induce chemotaxis of cultured microglia through Gi/o-coupled P2Y receptors. J Neurosci. 2001, 21 (6): 1975-1982.

  21. 21.

    la Sala A, Ferrari D, Di Virgilio F, Idzko M, Norgauer J, Girolomoni G: Alerting and tuning the immune response by extracellular nucleotides. J Leukoc Biol. 2003, 73 (3): 339-343. 10.1189/jlb.0802418.

  22. 22.

    Di Virgilio F: Purinergic signalling in the immune system. A brief update. Purinergic Signal. 2007, 3 (1-2): 1-3. 10.1007/s11302-006-9048-5.

  23. 23.

    Zimmermann H: Biochemistry, localization and functional roles of ecto-nucleotidases in the nervous system. Prog Neurobiol. 1996, 49 (6): 589-618. 10.1016/0301-0082(96)00026-3.

  24. 24.

    Zimmermann H, Braun N: Extracellular metabolism of nucleotides in the nervous system. J Auton Pharmacol. 1996, 16 (6): 397-400. 10.1111/j.1474-8673.1996.tb00062.x.

  25. 25.

    Plesner L: Ecto-ATPases: identities and functions. Int Rev Cytol. 1995, 158: 141-214.

  26. 26.

    Robson SC, Sevigny J, Zimmermann H: The E-NTPDase family of ectonucleotidases: Structure function relationships and pathophysiological significance. Purinergic Signal. 2006, 2 (2): 409-430. 10.1007/s11302-006-9003-5.

  27. 27.

    Zimmermann H: 5'-Nucleotidase: molecular structure and functional aspects. Biochem J. 1992, 285 (Pt 2): 345-365.

  28. 28.

    Thompson LF, Eltzschig HK, Ibla JC, Van De Wiele CJ, Resta R, Morote-Garcia JC, Colgan SP: Crucial role for ecto-5'-nucleotidase (CD73) in vascular leakage during hypoxia. J Exp Med. 2004, 200 (11): 1395-1405. 10.1084/jem.20040915.

  29. 29.

    Deaglio S, Dwyer KM, Gao W, Friedman D, Usheva A, Erat A, Chen JF, Enjyoji K, Linden J, Oukka M: Adenosine generation catalyzed by CD39 and CD73 expressed on regulatory T cells mediates immune suppression. J Exp Med. 2007, 204 (6): 1257-1265. 10.1084/jem.20062512.

  30. 30.

    Kobie JJ, Shah PR, Yang L, Rebhahn JA, Fowell DJ, Mosmann TR: T regulatory and primed uncommitted CD4 T cells express CD73, which suppresses effector CD4 T cells by converting 5'-adenosine monophosphate to adenosine. J Immunol. 2006, 177 (10): 6780-6786.

  31. 31.

    Thammavongsa V, Kern JW, Missiakas DM, Schneewind O: Staphylococcus aureus synthesizes adenosine to escape host immune responses. J Exp Med. 2009, 206 (11): 2417-2427. 10.1084/jem.20090097.

  32. 32.

    Eichelberg K, Ginocchio CC, Galan JE: Molecular and functional characterization of the Salmonella typhimurium invasion genes invB and invC: homology of InvC to the F0F1 ATPase family of proteins. J Bacteriol. 1994, 176 (15): 4501-4510.

  33. 33.

    Soderman K, Reichard P: A nitrocellulose filter binding assay for ribonucleotide reductase. Anal Biochem. 1986, 152 (1): 89-93. 10.1016/0003-2697(86)90124-7.

  34. 34.

    Neu HC: The 5'-nucleotidase of Escherichia coli. I. Purification and properties. J Biol Chem. 1967, 242 (17): 3896-3904.

  35. 35.

    Bengis-Garber C, Kushner DJ: Purification and properties of 5'-nucleotidase from the membrane of Vibrio costicola, a moderately halophilic bacterium. J Bacteriol. 1981, 146 (1): 24-32.

  36. 36.

    de Aguiar Matos JA, Borges FP, Tasca T, Bogo MR, De Carli GA, da Graca Fauth M, Dias RD, Bonan CD: Characterisation of an ATP diphosphohydrolase (Apyrase, EC 3.6.1.5) activity in Trichomonas vaginalis. Int J Parasitol. 2001, 31 (8): 770-775. 10.1016/S0020-7519(01)00191-6.

  37. 37.

    Jesus JB, Lopes AH, Meyer-Fernandes JR: Characterization of an ecto-ATPase of Tritrichomonas foetus. Vet Parasitol. 2002, 103 (1-2): 29-42. 10.1016/S0304-4017(01)00576-3.

  38. 38.

    Torres CR, Vasconcelos EG, Ferreira ST, Verjovski-Almeida S: Divalent cation dependence and inhibition of Schistosoma mansoni ATP diphosphohydrolase by fluorosulfonylbenzoyl adenosine. Eur J Biochem. 1998, 251 (1-2): 516-521. 10.1046/j.1432-1327.1998.2510516.x.

  39. 39.

    Schulte am Esch J, Sevigny J, Kaczmarek E, Siegel JB, Imai M, Koziak K, Beaudoin AR, Robson SC: Structural elements and limited proteolysis of CD39 influence ATP diphosphohydrolase activity. Biochemistry. 1999, 38 (8): 2248-2258. 10.1021/bi982426k.

  40. 40.

    Drosopoulos JH: Roles of Asp54 and Asp213 in Ca2+ utilization by soluble human CD39/ecto-nucleotidase. Arch Biochem Biophys. 2002, 406 (1): 85-95. 10.1016/S0003-9861(02)00414-9.

  41. 41.

    Drosopoulos JH, Broekman MJ, Islam N, Maliszewski CR, Gayle RB, Marcus AJ: Site-directed mutagenesis of human endothelial cell ecto-ADPase/soluble CD39: requirement of glutamate 174 and serine 218 for enzyme activity and inhibition of platelet recruitment. Biochemistry. 2000, 39 (23): 6936-6943. 10.1021/bi992581e.

  42. 42.

    Yang F, Hicks-Berger CA, Smith TM, Kirley TL: Site-directed mutagenesis of human nucleoside triphosphate diphosphohydrolase 3: the importance of residues in the apyrase conserved regions. Biochemistry. 2001, 40 (13): 3943-3950. 10.1021/bi002711f.

  43. 43.

    McMillen L, Beacham IR, Burns DM: Cobalt activation of Escherichia coli 5'-nucleotidase is due to zinc ion displacement at only one of two metal-ion-binding sites. Biochem J. 2003, 372 (Pt 2): 625-630.

  44. 44.

    Knofel T, Strater N: Mechanism of hydrolysis of phosphate esters by the dimetal center of 5'-nucleotidase based on crystal structures. J Mol Biol. 2001, 309 (1): 239-254. 10.1006/jmbi.2001.4656.

  45. 45.

    Lowy FD: Staphylococcus aureus infections. New Engl J Med. 1998, 339: 520-532. 10.1056/NEJM199808203390806.

  46. 46.

    Cheng AG, DeDent AC, Schneewind O, Missiakas DM: A play in four acts: Staphylococcus aureus abscess formation. Trends Microbiol. 2011,

  47. 47.

    Sendi P, Proctor RA: Staphylococcus aureus as an intracellular pathogen: the role of small colony variants. Trends Microbiol. 2009, 17 (2): 54-58. 10.1016/j.tim.2008.11.004.

  48. 48.

    Garzoni C, Kelley WL: Staphylococcus aureus: new evidence for intracellular persistence. Trends Microbiol. 2009, 17 (2): 59-65. 10.1016/j.tim.2008.11.005.

  49. 49.

    Scheffzek K, Ahmadian MR: GTPase activating proteins: structural and functional insights 18 years after discovery. Cell Mol Life Sci. 2005, 62 (24): 3014-3038. 10.1007/s00018-005-5136-x.

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Acknowledgements

We thank members of our laboratory for discussion and Dr. Justin Kern for assistance with circular dichroism. This work was supported in part by a grant from the National Institute of Allergy and Infectious Diseases (NIAID), Infectious Diseases Branch (AI52474). V.T. acknowledges support from the Immunology Training Grant at the University of Chicago (AI07090) and an AHA postdoctoral fellowship (10POST4590023). D.M.M. and O.S. are members of and supported by the Region V "Great Lakes" Regional Center of Excellence in Biodefense and Emerging Infectious Diseases Consortium (NIAID, NIH Award 1-U54-AI-057153).

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  1. Department of Microbiology, University of Chicago, 920 E. 58th St., Chicago, Illinois, 60637, USA

    • Vilasack Thammavongsa
    • , Olaf Schneewind
    •  & Dominique M Missiakas

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Correspondence to Dominique M Missiakas.

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VT defined the concept and conducted the experiments of the study. VT, OS, and DM drafted the manuscript. All authors have read and approved the final manuscript.

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Keywords

  • Circular Dichroism
  • Malachite Green
  • Extracellular Nucleotide
  • Nucleotidase Activity
  • Infiltrate Immune Cell

BMC Biochemistry

ISSN: 1471-2091

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