Figure 3
From: Enzymatic properties of Staphylococcus aureus adenosine synthase (AdsA)
AdsA is highly active at a near neutral pH and is dependent on metal cations. (A and B) Effect of pH on activity of rAdsA. Relative hydrolysis of 1 mm AMP (A) or 1 mm ADP (B) in buffer containing 50 mm Tris-HCl and 0.5 mM MnCl2 over the indicated pH range is shown. The results are expressed as the percentages of activity and are the average of three independent analyses. (C-D), Shown is the relative ADPase activity of AdsA in the presence of (C) MnCl2, (D) MgCl2, (E) ZnCl2, and (D) CuCl2. The results are expressed as relative activity of the highest value in each experiment. Data is the average of two independent analyses conducted in triplicate.