Figure 1

AdsA efficiently hydrolyzes adenosine nucleoside tri- and diphosphates. The ability of rAdsA to hydrolyze nucleoside tri- or diphosphates was evaluated by assessing inorganic phosphate released after incubation of rAdsA with the indicated substrates for 15 minutes. Percent activity is expressed as the amount of inorganic phosphate released relative to ATP (A and C, 914 ± 70 nmol pi) and ADP (B, 840 ± 55 nmol pi) within each experiment. The results are the average of 3 independent analyses conducted in duplicate and the error bars represent the SEM.