Engineering Klebsiella sp. 601 multicopper oxidase enhances the catalytic efficiency towards phenolic substrates
© Li et al; licensee BioMed Central Ltd. 2011
Received: 3 January 2011
Accepted: 31 May 2011
Published: 31 May 2011
Structural comparison between bacterial CueO and fungal laccases has suggested that a charged residue Glu (E106) in CueO replaces the corresponding residue Phe in fungal laccases at the gate of the tunnel connecting type II copper to the protein surface and an extra α-helix (L351-G378) near the type I copper site covers the substrate binding pocket and might compromise the electron transfer from substrate to type I copper. To test this hypothesis, several mutants were made in Klebsiella sp. 601 multicopper oxidase, which is highly homologous to E. coli CueO with a similarity of 90% and an identity of 78%.
The E106F mutant gave smaller K m (2.4-7fold) and k cat (1-4.4 fold) values for all three substrates DMP, ABTS and SGZ as compared with those for the wild-type enzyme. Its slightly larger k cat /K m values for three substrates mainly come from the decreased K m . Deleting α-helix (L351-G378) resulted in the formation of inactive inclusion body when the mutant Δα351-378 was expressed in E. coli. Another mutant α351-380M was then made via substitution of seven amino acid residues in the α-helix (L351-G378) region. The α351-380M mutant was active, and displayed a far-UV CD spectrum markedly different from that for wild-type enzyme. Kinetic studies showed the α351-380M mutant gave very low K m values for DMP, ABTS and SGZ, 4.5-, 1.9- and 7-fold less than those for the wild type. In addition, k cat /K m values were increased, 9.4-fold for DMP, similar for ABTS and 3-fold for SGZ.
The Glu residue at position 106 appears not to be the only factor affecting the copper binding, and it may also play a role in maintaining enzyme conformation. The α-helix (L351-G378) may not only block access to the type I copper site but also play a role in substrate specificities of bacterial MCOs. The α351-380M mutant catalyzing oxidation of the phenolic substrate DMP effectively would be very useful in green chemistry.
Multicopper oxidases (MCOs) contain a multiple copper center, and catalyze the oxidation of a variety of substrates along with the four-electron reduction of dioxygen to water . Laccases are a member of family , and are widely distributed in plants and fungi [2, 3]. Owing to their involvement in the transformation of a wide variety of phenolic compounds, including the polymeric lignin and humic substrates, fungal laccases attract considerable attention. It has been reported that laccases show multiple functions, including lignin degradation, pigmentation, lignin biosynthesis and pathogenesis in fungi, and, in addition to their organic substrate, require only molecular oxygen for catalysis, which makes them suitable for biotechnological application [4–11].
In our previous study, the gene encoding a MCO in a soil bacterium Klebsiella sp. 601 was cloned, the enzyme, with a mass of 58.2 kDa was purified and a preliminary survey of the enzymatic properties of the recombinant MCO was carried out . The amino acid sequence of Klebsiella sp. 601 MCO is highly homologous to that of E. coli CueO with a similarity of 90% and a 78% identity. High homology in amino acid sequences indicates two enzymes are likely to share essentially the same three-dimensional molecular structure. In this study, five mutants have been made via either amino acid deletion or replacement at E106 and the α-helix (L351-G378) region. Apart from three mutants expressing as inactive inclusion bodies, the mutants E106F and α351-380M with oxidase activities have been purified and studied enzymatically. Compatible with the result obtained in E. coli CueO, replacement of E106 by a Phe in Klebsiella sp. 601 MCO suggests that E106 is not the only factor affecting the copper binding. Alteration of amino acid residue in the mutant α351-380M changes the secondary structure of the α-helix, and significantly improves oxidase activity towards phenolic substrate DMP.
Overexpression and purity of mutant enzymes
Protein expression of Klebsiella sp. 601 MCO and 5 mutants in E. coli
Expression in E.coli
Active in supernatant
Protein in pellet
Comparison of kinetic parameters for three laccase substrates between two mutants and wild type of Klebsiella sp. 601 MCO
2,847 ± 30
1,176 ± 47
639 ± 17
37,800 ± 32
37,480 ± 886
79,913 ± 944
539 ± 5.0
159 ± 1.0
284 ± 6.2
68,409 ± 342
32,595 ± 152
35,990 ± 704
35,990 ± 704
11.67 ± 0.2
1.57 ± 0.02
1.46 ± 0.02
10.8 ± 0.1
2.43 ± 0.02
4.3 ± 0.05
A Ni-affinity column was used for purifying the mutant and wild-type enzymes to minimize the risk of CueO contamination from E. coli host cells. After purification by Ni-affinity chromatography, the enzymes purified from the mutants E106F and α351-380M and the wild-type MCO exhibited a single band on the SDS gel with a molecular mass of about 60 kDa, similar to that (58.2 kDa) reported for wild-type enzyme  (see Figure 2b). About 15 mg E106F, 8 mg α351-380M and 20 mg wild-type pure enzymes were obtained respectively from 1L cultures of E. coli BL21(DE3)pLysS transformants after the purification described.
Comparison of kinetic parameters between two mutant enzymes and wild-type MCO
Enzyme activities of two mutants and wild-type MCO were examined with a fixed concentration of DMP (1 mM), ABTS (0.5 mM) or SGZ (0.01 mM) over a range of pH values: the optimal pHs for the catalysis of two mutants were pH5.0 for DMP, pH3.6 for ABTS and pH6.8 for SGZ. The wild-type enzyme also gave the highest activities at pH 5.0 for DMP, pH3.0 for ABTS and pH6.8 for SGZ.
Kinetic parameters were estimated under the conditions described in the experimental section by varying the DMP (0.2-5.0 mM), ABTS (0.05-1.0 mM) and SGZ (0.6-20 μM) concentrations. The results are summarized in Table 2. As compared to the wild type, the K m value of E106F for DMP at pH5.0 is 2.4-fold low and the k cat value remains comparable. The decreased Km value results in a 2.4-fold increase in the catalytic coefficient (k cat /K m ) in mutant E106F. The K m value for the α351-380M mutant is 4.5-fold smaller, and the k cat and k cat /K m values are 2.1-fold and 9.4-fold larger respectively, as compared with those for the wild-type enzyme. The large catalytic coefficient with a low K m value indicates the mutant α351-380M favors DMP as its substrate.
Kinetic parameters of two mutants for ABTS were also monitored at pH3.6. Both E106F and α351-380M mutants gave the K m values of 159 and 284 μM, 3.4- and 1.9-fold smaller than that (539 μM) for the wild-type enzyme at pH3.0. Nevertheless, the catalytic coefficients for both mutants are very close to that for the wild-type enzyme due to their low k cat values. Kinetic parameters for anther substrate SGZ were also estimated at pH6.8. Both mutants gave similar K m values for SGZ, 7-fold smaller than that for wild-type enzyme. However, again, k cat /K m values for both mutants are only 1.7- and 3-fold larger than that for the wild-type enzyme because of their low k cat values. Table 2 also shows that the wild-type enzyme, similar to the mutant E106F, catalyzes oxidation of ABTS much more effectively than the other two substrates DMP and SGZ. In contrast, the α351-380M mutant uses DMP as effectively as ABTS based on the values of k cat /K m obtained.
Far-UV CD spectra of the two mutant enzymes
Based on the information up to date, bacterial multicopper oxidases possess much lower oxidative activities compared to fungal laccases, although structure comparison between bacterial and fungal laccases shows the overall structure and copper coordination are very similar. Nevertheless, a charged residue Glu (E106) in CueO replaces the corresponding residue Phe in fungal laccases at the gate of the tunnel connecting type II copper to the protein surface . Li et al (2007) have suggested the hydrophobic residue Phe in fungal laccases likely gates the tunnel and prevents type II copper exchange from solvent, while the charged residue Glu in CueO would make type II copper solvent accessible and easy to be lost . According to this hypothesis, replacement of E106 by a Phe residue should enhance oxidase activities of Klebsiella sp. 601 MCO. Kinetic data collected from individual enzyme assay towards DMP, ABTS or SGZ shows that the E106F mutant has smaller K m and k cat values but larger k cat /K m values for all three substrates as compared with those for the wild-type enzyme (see Table 2). Its larger k cat /K m values for three substrates, which mainly comes from its small K m , indicate the mutant E106F catalyzes DMP, ABTS or SGZ more effectively than the wild-type enzyme. Although the Km value may not really represent binding constant for its substrate to an enzyme, smaller K m values for three substrates imply that E106 is not to be the only factor affecting the copper binding. Given that E106 is far from the substrate binding site, it is difficult to explain why E106F should have decreased K m values for all three substrates. Easy access of three substrates to the Cu I site in the active pocket may imply a possibly conformational change of this active pocket in the mutant enzyme. This may also offer us an explanation for the combined mutant E106F/α351-380M expressed in E. coli as inactive inclusion body owing to improper folding.
In the proposed mechanism of laccase catalysis, electrons are first transported from substrate to type I copper, and then travel through a 13 Å pathway formed by a cysteine and two histidines to type III copper, and finally reduce molecular oxygen to water at type II copper . According to this mechanism, substrates should bind near the type I copper. In the molecular model of the complex of B. subtilis CotA and substrate, the ABTS-binding pocket is indeed very close to the type I copper site . Kataoka et al (2007) also noticed that the substrate-binding site of CueO is buried deeply under a methionine-rich helical region including α-helices 5, 6 and 7, which is different from other MCOs . They deleted the methionine-rich region Pro357-His406 and then replaced it with a Gly-Gly linker. The cuprous oxidase activity of the truncated mutant decreases to10% that of the wild-type enzyme, but the activities for laccase substrates ABTS and DMP are very similar in the presence of Cu(II) ions . 50 amino acid residues replaced by a Gly-Gly linker may perhaps result in too much flexibility of the peptide chain containing rich Gly residues in solvent so that substrates may not easily gain access to the active pocket of the mutated enzyme. Detailed structural comparison has shown that the substrate binding pockets in fungal laccases are fully open and type I copper is exposed to solvent. In contrast, in the bacterial CueO, an extra α-helix (L351-G378) is located over type I copper and makes the substrate binding pocket smaller . To test this hypothesis in the present study, a mutant Δα351-378 was first created by deleting 28 amino acids of the α-helix. The mutated protein was expressed in the form of inactive inclusion bodies in the pellet, similar to the result obtained in E. coli CueO . The deletion of α-helix may result in improper folding of the Δα351-378 mutant. To further examine if the α-helix from L351 to G378 affects substrate binding, the mutant α351-380M was made through substitution of seven amino acid residues in the α-helix. Far-UV CD spectrum suggests that the replacement of seven amino acids appears to abolish the secondary structure of the α-helix between L351 G378. The α351-380M mutant is active towards all three substrates tested with very small K m values, 2-7 fold lower than those for the wild-type enzyme (see Table 2). Decreased K m values indicate that the α-helix from L351 to G378 indeed is a barrier for substrate binding to the enzyme. As compared to the wild-type enzyme, the α351-380M mutant gives a 2-fold lower k cat with a similar k cat /K m values for ABTS, a 2-fold lower k cat with a 3-fold larger k cat /K m value for SGZ, and a 2-fold larger k cat with a 10-fold larger k cat /K m value for DMP. Smaller K m , larger k cat and k cat /K m for DMP demonstrate that structural modification at α351-378 markedly enhances oxidase activity of Klebsiella sp. 601 MCO towards phenolic substrate DMP. The wild-type MCO favors ABTS as substrate, whereas the α351-380M mutant catalyzes the substrate DMP as effectively as ABTS, demonstrating that the α351-378 shifts its substrate specificity towards DMP. Our results suggest the α-helex may also play a role in substrate specificities of bacterial MCOs.
Laccases are very attractive enzymes for application in biotechnology and green chemistry. At present, only fungal laccases are used in industrial processes. However, fungal laccases are much more difficult to express as active enzymes in bacterial or yeast hosts to meet industrial requirement. Bacterial laccases perhaps are another candidate for industry. 10-fold increase of substrate specificity offers an opportunity to use the α351-380M mutant for biotechnological application. Its low soluble expression in E. coli will be improved in the near future by periplasmic protein expression, random mutagenesis or incubating under low temperature plus microaerobic conditions, because these strategies have been recently demonstrated to be a very useful in B. licheniformis and Bacillus sp. HR03 CotA [25, 26]. The α351-380M mutant is an example of the successful modification of MCO function to a large extent, and extensive engineering of bacterial laccases is expected to create more novel functions.
In summary, the data obtained from kinetic analysis of two mutants shows that (1) the glutamate residue at position 106 appears not to be the only factor affecting the copper binding, and it may also play a role in maintaining enzyme conformation correctly; and (2) the α-helix (L351-G378) may not only block access to the type I copper site but also play a role in substrate specificities of bacterial MCOs. The α351-380M mutant catalyzing oxidation of the phenolic substrate DMP effectively would be very useful in green chemistry.
Materials and methods
Materials and chemicals
KOD plus taq DNA polymerase, dNTP and Dpn1 were obtained from Takara and Toyoba (Japan). Laccase substrates 2,6-dimethylphenol (MDP), 2,2'-azino-bis-(3-ethylbenzibiozoline 6-sulfonic acid) (ABTS) and 4-hydroxy-3,5- dimethoxybenzaldehyde azine (SGZ) were acquired from Sigma (USA). Oligonucleotides for mutagenesis and DNA sequencing were made by Invitrogen (Shanghai). All chemicals were of analytical grade.
Bacterial strains and vectors
The E. coli strains used in mutagenesis and gene expression were DH5α (F- endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZ ΔM15 Δ(lacZYA-argF)U169, hsdR17(rK- mK+), λ-) and BL21(DE3)pLysS (F- ompT gal dcm lon hsdSB(rB- mB-) λ(DE3) pLysS(cmR)), both from Promega Biotech Co. The recombinant plasmid pET23a-601 harbouring Klebsiella sp. 601 MCO gene was constructed by Ms. Jiao Yin.
Mutagenesis of Klebsiella sp. 601 MCO gene
Oligonucleotide primers used for mutating Klebsiella sp. 601 MCO gene
5'-CCTCGCCCGGGACAAA CAGGCCATGCCAGTG -3'
5'- GGGCTGACGCAGCGTCAG ATGGACCACGGCATGATGGA -3'
5'-CTGACGCTGCGTCAGCCC CGTCAGGGACGGT -3'
5'-GATCCGATGCTCGACGGAGGA GGCATGCAGGCGGGAGGACCT AAGTATGGC GACCAG-3'
5'-CTGGTCGCCATACTTAGGTCCTCC CGCCTGCATGCCTCCTCC GTCGAGCAT CGGATC-3
5'-TATGGCGACCAGGCGAAA GCGGGACCT GACCACGGCATGATG -3'
5'-CATCATGCCGTGGTCAGG TCCCGCTTT CGCCTGGTCGCCATA -3'
In this study, five mutants were finally obtained through one, two or three rounds of PCR and Dpn I digestion as described above. In the mutant E106F, the phenylalanine codon (TTT) replaced the wild-type glutamate (GAG). To investigate if the α-helix (L351-G378) affects substrate access to the type I copper site, the mutant Δα351-378 was first created by deleting all 28 amino acids in the α351-378 region of wild-type enzyme. Then the mutant α351-380M was also made via substitution of 361R, 362M, 367L, 368M, 369E, 376M, 379M with a glycine, glycine, glycine, glycine, proline, lysine and proline, respectively. The point mutant E106F was also combined with the mutant Δα351-378 and α351-380M to form two other mutants E106F/Δα351-378 and E106F/α351-380M. All mutations were confirmed by sequencing the overall DNA of each mutated gene.
The recombinant plasmids with the mutated MCO genes were extracted from E. coli DH5α, and then transformed into E. coli BL21(DE3)pLysS. Positive transformants of E. coli BL21(DE3)pLysS were grown at 23°C in LB broth containing 100 μg/ml ampicillin and induced by adding 0.5 mM isopropyl β-D-thiogalactopyranoside (IPTG) into the culture when OD600 was equal to 0.6. Bacterial cells were harvested and sonicated, and crude extracts were prepared through centrifugation at 27,000 g for 15 min.
Each mutant enzyme was purified by Ni-affinity chromatography, as described for the cloned wild-type MCO . The clarified crude extract was loaded onto the Ni-column equilibrated with 100 mM phosphate (pH7.4). The column was washed with 100 mM phosphate (pH7.4) and then eluted with a gradient of 20-150 mM imidazole in 100 mM phosphate (pH7.4). Fractions were collected automatically with 1 ml per tube. Enzyme purity was routinely examined by SDS-PAGE on 15% gels, and the concentration of purified enzyme was estimated from A280 on the basis of the absorption coefficient of 1.03 mg ml-1 predicted by the program ProtParam (http://expasy.org/tools/protparam.html). The pure enzyme, precipitated in 65% ammonium sulfate and stored at 4°C, was dialyzed before use against several changes of 100 mM phosphate buffer (pH7.4), and clarified by centrifugation.
Enzyme activity in crude extract was initially examined using activity staining on 10% native gel, as described by Li et al . The gel was first soaked in 50 mM acetate buffer (pH5.0) for 10 min, and then stained in 2.5 mM DMP, 0.2 mM CuSO4 and 50 mM H3PO4-Na2HPO4 (pH5.0) at room temperature for 10-15 min. Enzyme activity was also examined spectrophotometrically at 37°C by recording the change in A447 in the assay mixture containing 1 mM DMP, 0.2 mM CuSO4, 50 mM acetic acid-sodium acetate (pH5.0) and 10 μl crude extract.
Specific activity was measured with a Shimadzu UV/visible spectrophotometer (UV-2550). The extinction coefficients used in active assays were 14.8 mM-1 cm-1 for DMP, 36 mM-1 cm-1 for ABTS and 65 mM-1 cm-1 for SGZ. Oxidation of DMP was assayed at 37°C in 50 mM acetate (pH5.0), of ABTS in 50 mM acetic acid-sodium acetate (pH3.0 or 3.6), and of SGZ in 50 mM phosphate (pH6.8). In addition, 0.2 mM CuSO4 was routinely added into the reaction solution. Specific activities were expressed as units of activity per milligram of protein, where one unit represented a micromole of substrate oxidized per minute. To determine kinetic parameters, rates were measured spectrophotometrically at 37°C with 0.2 mM CuSO4 over a range of substrate concentrations (for DMP 0.2-5.0 mM, ABTS 0.05-1.0 mM and SGZ 0.6-20 μM). Michaelis-Menten parameters were calculated using the UVProbe-[Kinetics] version 1.11a (SHIMADZU Corporation), and kinetic parameters were determined by Lineweaver-Burk plot  and also checked by Hanes-Woolf and Eddie-Hofstee plots. The deviation between the same parameters obtained from different plots was less than 5%. In each plot, correlation coefficient (r2) value was equal to or large than 0.997. All data were also analyzed using the statistic software SPSS based on the non-linear regression method INVERSE, and analysis of variance gave P values of less than 0.005 in each case.
We thank Prof. Paul C Engel of Conway Institute, University College Dublin, Republic of Ireland, for his helps with review of the manuscript and English editing, and Mr. Wenfeng Zuo for his kindly technical supports. These studies were financially supported by Hubei Provincial Department of Education (2004Z001 to XGW) and Wuhan Science and Technology Bureau (201160823257 to YL).
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