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Table 1 Protein expression of Klebsiella sp. 601 MCO and 5 mutants in E. coli

From: Engineering Klebsiella sp. 601 multicopper oxidase enhances the catalytic efficiency towards phenolic substrates

Mutation Expression in E.coli Active in supernatant Protein in pellet
Wild type + + +
E106F + + ++
Δα351-378 + - ++++
E106F/Δα351-378 + - ++++
α351-380M + + +++
E106F/α351-380M + - ++++
  1. Enzyme activity in supernatant was measured at 37°C by recording the change in A447 in 1 ml assay mixture containing 1 mM DMP, 0.2 mM CuSO4, 50 mM acetic acid-sodium acetate (pH5.0) and 10 μl crude extract. +: positive; -: negative.