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Table 1 Protein expression of Klebsiella sp. 601 MCO and 5 mutants in E. coli

From: Engineering Klebsiella sp. 601 multicopper oxidase enhances the catalytic efficiency towards phenolic substrates

Mutation

Expression in E.coli

Active in supernatant

Protein in pellet

Wild type

+

+

+

E106F

+

+

++

Δα351-378

+

-

++++

E106F/Δα351-378

+

-

++++

α351-380M

+

+

+++

E106F/α351-380M

+

-

++++

  1. Enzyme activity in supernatant was measured at 37°C by recording the change in A447 in 1 ml assay mixture containing 1 mM DMP, 0.2 mM CuSO4, 50 mM acetic acid-sodium acetate (pH5.0) and 10 μl crude extract. +: positive; -: negative.