Mutation | Expression in E.coli | Active in supernatant | Protein in pellet |
---|
Wild type | + | + | + |
E106F | + | + | ++ |
Δα351-378 | + | - | ++++ |
E106F/Δα351-378 | + | - | ++++ |
α351-380M | + | + | +++ |
E106F/α351-380M | + | - | ++++ |
- Enzyme activity in supernatant was measured at 37°C by recording the change in A447 in 1 ml assay mixture containing 1 mM DMP, 0.2 mM CuSO4, 50 mM acetic acid-sodium acetate (pH5.0) and 10 μl crude extract. +: positive; -: negative.