Table 3 Oligonucleotide primers used for mutating Klebsiella sp. 601 MCO gene
Oligo name | Sequence |
|---|---|
106F-F | 5'-ACTGGCATGGCCTGTTT GTCCCGGGCGAGGTC-3' |
106F-R | 5'-CCTCGCCCGGGACAAA CAGGCCATGCCAGTG -3' |
Δα351-378- F | 5'- GGGCTGACGCAGCGTCAG ATGGACCACGGCATGATGGA -3' |
Δα351-378- R | 5'-CTGACGCTGCGTCAGCCC CGTCAGGGACGGT -3' |
α351-380M-F1 | 5'-GATCCGATGCTCGACGGAGGA GGCATGCAGGCGGGAGGACCT AAGTATGGC GACCAG-3' |
α351-380M-R1 | 5'-CTGGTCGCCATACTTAGGTCCTCC CGCCTGCATGCCTCCTCC GTCGAGCAT CGGATC-3 |
α351-380M-F2 | 5'-TATGGCGACCAGGCGAAA GCGGGACCT GACCACGGCATGATG -3' |
α351-380M-R2 | 5'-CATCATGCCGTGGTCAGG TCCCGCTTT CGCCTGGTCGCCATA -3' |