- Research article
- Open Access
1,2-Dichlorobenzene affects the formation of the phosphoenzyme stage during the catalytic cycle of the Ca2+-ATPase from sarcoplasmic reticulum
© Vargas-Medrano et al. 2016
- Received: 3 September 2015
- Accepted: 2 March 2016
- Published: 11 March 2016
1,2-Dichlorobenzene (1,2-DCB) is a benzene-derived molecule with two Cl atoms that is commonly utilized in the synthesis of pesticides. 1,2-DCB can be absorbed by living creatures and its effects on naturally-occurring enzymatic systems, including the effects on Ca2+-ATPases, have been poorly studied. Therefore, we aimed to study the effect of 1,2-DCB on the Ca2+-ATPase from sarcoplasmic reticulum (SERCA), a critical regulator of intracellular Ca2+ concentration.
Concentrations of 0.05–0.2 mM of 1,2-DCB were able to stimulate the hydrolytic activity of SERCA in a medium-containing Ca2+-ionophore. At higher concentrations (0.25–0.75 mM), 1,2-DCB inhibited the ATP hydrolysis to ~80 %. Moreover, ATP hydrolysis and Ca2+ uptake in a medium supported by K-oxalate showed that starting at 0.05 mM,1,2-DCB was able to uncouple the ratio of hydrolysis/Ca2+ transported. The effect of this compound on the integrity of the SR membrane loaded with Ca2+ remained unaffected. Finally, the analysis of phosphorylation of SERCA by [γ-32P]ATP, starting under different conditions at 0° or 25 °C showed a reduction in the phosphoenzyme levels by 1,2-DCB, mostly at 0 °C.
The temperature-dependent decreased levels of phosphoenzyme by 1,2-DCB could be due to the acceleration of the dephosphorylation mechanism – E2P · Ca2 state to E2 and Pi, which explains the uncoupling of the ATP hydrolysis from the Ca2+ transport.
- Sarcoplasmic reticulum
1,2-Dichlorobenzene (1,2-DCB) is a chlorinated benzene molecule used as precursor in the synthesis of pesticides. 1,2-DCB can be absorbed and accumulated by humans and wildlife, and it has been detected in different biological fluids. Moreover, 1,2-DCB has been linked to severe human health problems such as liver damage and anemia [1–6]. Oral administration of a radioactively-labeled 1,2-DCB to rats revealed that it can be distributed among all tissues, including skeletal muscle. In addition, it has been shown that DCB molecules can induce a rise in intracellular Ca2+ in human neuronal SH-SY5Y cells [7, 8]. Given this evidence, we hypothesized that 1,2-DCB can affect the functionality of skeletal muscle proteins, particularly the Ca2+-ATPase from sarcoplasmic reticulum (SERCA), a predominant protein of skeletal muscle, a hypothesis that has not previously been explored. SERCA is a large (110-kDa) sarcoplasmic reticulum protein with 10 transmembrane regions, and it plays the essential role of reducing the intracellular Ca2+ concentration by consuming ATP as a source of energy [9–12]. Each molecule of SERCA protein utilizes the energy of one ATP molecule to pump 2Ca2+ across the sarcoplasmic membrane, thus maintaining low cytoplasmic Ca2+ concentrations and high intravesicular Ca2+ concentrations [13, 14]. The catalytic cycle of SERCA involves two main conformational states, E1 with high-Ca2+ and E2 with low-Ca2+ affinities [15–17]. In addition, the three-dimensional structures of the two conformational stages of SERCA and phosphorylated intermediate (EP) have been successfully determined [18–22]. Furthermore, the coupling ratio of both transported Ca2+ and hydrolyzed ATP occurs through a sequence of phosphorylated and non-phosphorylated enzymatic intermediates, with or without Ca2+ bound to the enzyme, and it has been shown that EP accumulation can serve as an indicator of enzyme turnover, which can be affected by a variety of factors such as temperature, nature of the substrate, Ca2+ concentration and presence of organic solvents .
Given the critical role of SERCA in maintaining appropriate cytosolic Ca2+ levels, chemical agents that affect its functionality can have harmful effects to the overall cellular function. In this regard, a multitude of molecules have been described to act as inhibitors of SERCA . Naturally-occurring compounds such as cyclopiazonic acid (a fungal toxin), and thapsigargin (a sesquiterpene lactone) are two of the most widely used inhibitors of SERCA [25–27]. Inorganic compounds such as vanadate, tungstate and molybdate have also been reported to inhibit SERCA activity [28–31]. Other natural compounds such as ellagic acid and gingerol, which have been described to stimulate SERCA activity, have been shown to be promising therapeutic targets for cardiovascular dysfunction [32, 33]. In addition, several molecules, including thapsigargin derivatives and flavonoids are being developed as potential cancer treatments based on their SERCA-mediated anti-proliferative and pro-apoptotic effects [34, 35]. Furthermore, a broad variety of organic solvents and hydrophobic compounds bearing nucleophilic groups have been extensively reported as inhibitors of SERCA activity [36–41]. The inhibitory effect of these compounds was related to their hydrophobicity, and notably, they were also able to stimulate SERCA activity at certain concentrations. In this study, we provide evidence, for the first time, that 1,2-DCB affects Ca2+ homeostasis by affecting SERCA, a fundamental Ca2+ modulator. We report here the effect of 1,2-DCB on several key features of SERCA, including ATP hydrolytic activity, the ratio of hydrolysis of ATP/Ca2+ transported, and the phosphorylation stage (EP) during the catalytic cycle of this enzyme.
Chemicals and reagents
[γ-32P]ATP and 45CaCl2 were purchased from Perkin-Elmer, USA. [3H]Glucose and 45CaCl2 were products of DuPont NEN. The Ca2+ ionophore A23187 (calcimycin), EGTA (ethylene glycol-bis (β-aminoethyl ether)-N,N,N’,N’-tetra acetic acid), Mops (4-morpholinepropanesulfonic acid), liquid scintillation cocktail (Sigma-Fluor S-4023), ATP disodium salt, LaCl3, K-oxalate, benzene, 1,2-DCB was obtained from Sigma, Co. (St. Louis, MO). CaCl2 and other chemicals ware purchased from J.T. Baker (México). The Ca2+ ionophore A23187 was dissolved in ethanol. Benzene and 1,2-DCB were dissolved in methanol. Ethanol and methanol never reached a concentration higher than 1 % (v/v) in the reaction media after the addition of 1,2-DCB or A23187.
Preparation of sarcoplasmic vesicles
SR (sarcoplasmic reticulum) membranes rich in Ca2+-ATPase (SERCA) were prepared from the low density SR of skeletal fast-twitch muscle of adult New Zealand rabbit hind limbs, and preserved in a buffer containing 10 mM Mops, pH 7.0 and 30 % sucrose and stored at −80 °C as described by Eletr and Inesi . The sarcoplasmic membrane concentration refers to milligrams (mg) of total protein per milliliters (ml) and was measured by the colorimetric procedure described by Lowry et al. . We used bovine serum albumin as standard protein.
Free Ca2+ concentration in the reaction media
Free Ca2+ concentration was adjusted by adding the appropriate volume of CaCl2 and EGTA to the enzymatic reaction media . Free Ca2+ concentration was calculated by the computer program Calcium which is based on the absolute constant stability for the Ca2+-EGTA complex, the EGTA protonation equilibrium, Ca2+ ligands and media pH [45, 46].
Ca2+-ATPase (SERCA) hydrolytic activity supported by A23187 or K-oxalate
Initial rates of ATP hydrolysis by SR vesicles were measured at 25 °C for 5 min (min) by following the liberation of inorganic phosphate (Pi). A typical assay medium was buffered at pH 7.0, and contained 20 mM Mops, pH 7.0, 80 mM KCl, 5 mM MgCl2, 1 mM EGTA, 0.967 mM CaCl2 (10 μM free Ca2+),0.01 mg/ml SR vesicles, 1.5 μM A23187 (to generate leaky vesicles to measure in min) or 5 mM K-oxalate (no leaks and to measure in min). A typical reaction was started after the addition of 1 mM of ATP. Any change in the concentration of SR protein added to the reaction medium is detailed in the figure captions. The compound 1,2-DCB was added at the indicated concentrations (0.05–1 mM). The appearance of Pi from the hydrolysis of ATP mediated by SERCA was evaluated with a molybdovanadate reagent previously described by Lin and Morales . Experimental details are indicated in the corresponding figure legends.
Ca2+ uptake supported by K-oxalate
SERCA is a Ca2+ pump that depends on Mg2+ and the presence of ATP to transport Ca2+ across of the SR membrane. For this reason, we studied the Ca2+ transported by SERCA in the presence of 1,2-DCB. Ca2+ transported by SERCA was measured at 25 °C using 45CaCl2 as a radioactive tracer followed by sample filtration according with the methods described by Martonosi and Feretos . The experiment was conducted in a reaction mixture containing 20 mM Mops, pH 7.0, 80 mM KCl, 5 mM MgCl2, 1 mM EGTA, 5 mM K-oxalate instead of ionophore A23187 (to keep vesicles intact), 0.967 mM CaCl2 (10 μM free Ca2+) with 1000 cpm 45CaCl2 per nmol of Ca2+, and different concentrations of 1,2-DCB. The reaction contained 0.01 mg/ml of protein and was started after adding 1 mM of ATP. Ca2+ uptake mediated by SERCA was stopped by filtering the vesicles in 0.45 μm HA type nitrocellulose membranes filters (Millipore, Milford, MA). The filters containing the Ca2+-loaded SR vesicles were washed two times with 2 ml of a buffer containing 20 mM Mops pH 7.0, 80 mM KCl, 5 mM MgCl2 and 1 mM LaCl3. Finally, 45Ca2+ transported was measured by scintillation spectroscopy using 3 ml of scintillation cocktail per vial. The use of radioactive standards allowed us to express the Ca2+ uptake data as nmol Ca2+/mg of protein.
Membrane integrity test
In order to determine if 1,2-DCB was affecting the membrane integrity and therefore interfering with Ca2+ measurements, SR vesicles (0.01 mg/ml of protein) were loaded with 45Ca2+ by performing a Ca2+ transport assay using the active transport of SERCA as described before for Ca2+ uptake . The reaction was started with 1 mM of ATP and the reaction ran for 5 s (s) until SR vesicles were loaded with detectable amounts of Ca2+. In order to determine if DCB induced vesicle leakage, the 45Ca2+-loaded vesicles were exposed to 1 mM of a 1,2-DCB right before stopping the reaction by filtration. In addition, two positive controls were incorporated in substitution for oxalate: Ca2+ ionophore (1.5 μM of A23187) in combination with 1 mM 1,2-DCB, and Ca2+ ionophore alone. Very importantly, A23187 does not allow Ca2+ to be accumulated into SR vesicles. For more details of the experiment please review Ca2+ uptake supported by oxalate above. The amount of 45Ca2+ loaded into the vesicles was determined by scintillation spectroscopy.
Ca2+ binding at equilibrium assay
The binding of Ca2+ to SERCA was tested in the absence of ATP using 45CaCl2. Unbound Ca2+ was evaluated from the filter wet volume by using [3H]glucose as a marker. This approach was used to test the Ca2+ high-affinity conformational state of SERCA (E1Ca2 stage). The effect of 1,2-DCB on E1Ca2 state was evaluated at 25 °C for 5 min in a Ca2+-saturating medium containing 20 mM Mops, pH 7.0, 5 mM MgCl2, 80 mM KCl, 0.1 mM EGTA, 0.105 mM [45Ca]CaCl2 (~5000 cpm/nmol) (10 μM free Ca2+), 1 mM [3H]glucose (~10,000 cpm/nmol), and 0.2 mg/ml of SR protein. Subsequently, we added different concentrations of 1,2-DCB. In order to test the effect of 1,2-DCB on E2 stage we used a Ca2+-free media containing 20 mM Mops, pH 7.0, 5 mM MgCl2, 80 mM KCl, 0.1 mM EGTA, 1 mM [3H]glucose (~10,000 cpm/nmol), and 0.2 mg/ml of SR protein, and different concentrations of 1,2-DCB. The incubation was performed at 25 °C for 5 min. Later, the reaction for E2 stage was supplemented by adding a buffer containing 20 mM MOPS, pH 7.0, 5 mM MgCl2, 80 mM KCl, 0.1 mM EGTA, 3.15 mM [45Ca]CaCl2 (~5000 cpm/nmol) and 1 mM [3H]glucose (~10,000 cpm/nmol) with a final free-Ca2+ concentration of 10 μM. Following, the assay was prolonged for 1 min at 25 °C. Samples of 1 ml (0.2 mg of protein) were filtered under vacuum in 0.45 μm nitrocellulose filters (Millipore, HA type). Any further washing to determine 45Ca and 3H associated with the filters and the amount of radioactivity in the filters was determined by scintillation spectroscopy. Specific binding of Ca2+ to the SERCA was calculated by subtracting unbound Ca2+ retained by the filter.
Phosphorylation of SERCA by [γ32]ATP
Maximal levels of EP (phosphorylated enzyme intermediate) were determined using [γ-32P]ATP as a substrate and starting from different incubation conditions: E1Ca2 -DCB, E2 -DCB and E2[γ-32P]ATP-DCB in similar manner as we described in previous work [25, 26]. These conditions were studied during the enzyme turnover by mixing equal volumes (0.5 ml) of SR vesicles with the phosphorylating medium with or without Ca2+ (after both solutions were mixed, the final volume was 1 ml and 0.1 mg/ml of SR protein). The SR vesicles (0.2 mg protein/ml) were suspended in a tube with 0.5 ml of 20 mM Mops, pH 7.0, 80 mM KCl, 5 mM MgCl2, 1 mM EGTA, 0.967 mM CaCl2 (10 μM free Ca2+), and 15 μM A23187. To measure E1Ca2-DCB stage, the reaction mixture was preincubated with a defined concentration of 1,2-DCB and the phosphorylation reaction was started by mixing 0.5 ml of this medium with 20 μl of medium containing 20 mM Mops, pH 7.0, 80 mM KCl, 5 mM MgCl2, 1 mM EGTA, 0.967 mM CaCl2, and 1.25 mM [γ-32P]ATP (50 μM final concentration, ~20,000 cpm/nmol). When the enzyme (0.2 mg/ml) was in nominally Ca2+-free medium (E2 -DCB stage), the phosphorylating medium contained CaCl2 to give 10 μM free Ca2+ after mixing. In order to determine if 1,2-DCB was directly affecting the E2ATP in a Ca2+ free medium, the formation of the EP stage was started when Ca2+ (to give 10 μM free) was added into the reaction medium. Phosphorylation time was 2 s when the enzyme turnover was studied at 0 °C, and 5 s when studied at 25 °C. The reaction was started under continuous vortexing and stopped by adding 1 ml of ice-cold 250 mM perchloric acid and 2 mM sodium phosphate. Stopped reactions were incubated on ice for 5 min before filtration through 0.45 μm nitrocellulose filters (Millipore, HA type). SR vesicles retained by the filters were washed 5 times with ice-cold 250 mM perchloric acid and 2 mM sodium phosphate. Finally, the amount of radioactivity in the filters was determined by scintillation spectroscopy. These results are expressed as nmol EP/milligram of protein.
Statistical and kinetic analysis was performed using Sigma Plot 11 software. Unless otherwise stated, the experiment values are represented as the mean of at least three independent experiments, each performed in triplicate +/− average SE, with statistical significance of p < 0.05 determined by t-student’s paired t-test.
Effect of 1,2-DCB on the hydrolytic activity of SERCA supported by A23187
Effect of 1,2-DCB on Ca2+ uptake and ATP hydrolysis ratio mediated by SERCA and supported by K-oxalate
Membrane protein-dependent effect of 1,2-DCB and SR membrane integrity
Formation of EP stage during the catalytic cycle of SERCA was inhibited by 1,2-DCB
Our data show that 1,2-DCB exerts a dual response of stimulation and inhibition in SERCA hydrolytic activity supported by A23187 (Fig. 1). Similar effects of activation and inhibition of SERCA caused by other hydrophobic compounds have been reported in the literature, and possible explanations include alterations in the lipid environment of the enzyme, as well as accessing sensitive domains of the pump due to their hydrophobic properties [49–51]. The 1,2-DCB molecule possesses two Cl atoms in the benzene ring. In order to test the importance of the Cl atoms on the effect of this molecule on SERCA, we used benzene (which lacks Cl atoms) and we were able to avoid the inhibitory and stimulatory effects on SERCA activity produced by 1,2-DCB (Fig. 1). Our data supports a clear relation between the structure of 1,2-DCB and the effect produced on the ATP hydrolytic activity, since the absence of Cl atoms does not cause any effect on SERCA. Similar conclusions have been previously reported regarding the effects caused by the presence of electronegative groups [52–54]. Moreover, hydrophobic molecules can interact with hydrophobic domains in the membrane proteins, thus affecting enzyme functionality [37, 53, 55].
We also addressed the possibility of 1,2-DCB affecting the activity of binding/translocation of Ca2+ by SERCA. Surprisingly, a 1 mM concentration of 1,2-DCB fully inhibited Ca2+ uptake without affecting Ca2+-binding, which suggests that the binding of Ca2+ is not a major problem for the inhibition of Ca2+ transport. However, this is indicative that 1,2-DCB is affecting the Ca2+ sensitive conformation of the enzyme to a lesser extent (Figs. 2a and 3 respectively). Nevertheless, our data of binding of Ca2+ suggested that Ca2+ was able to bind to the enzyme in turnover conditions almost at normal levels, even when Ca2+ uptake was completely inhibited at the highest DCB concentration. Therefore, it was necessary to study the hydrolytic activity of SERCA under the same conditions used for the Ca2+ transport assay, using K-oxalate instead of the Ca2+ ionophore. When intravesicular Ca2+ levels get higher (~2–3 mM), Ca2+ reacts with oxalate to form calcium oxalate thus precipitating inside of the intact vesicle as crystals , which allows us to measure the enzyme activity in min. Surprisingly, we found that the hydrolytic activity of SERCA under these same conditions was still stimulated, but not inhibited at the same concentrations tested before (Fig. 3b). This may be due to the different experimental conditions such as the presence of ionophore, which allows the system to function more efficiently. The Ca2+-ionophore acts as a freely mobile carrier that transports Ca2+ across the membrane, and it is possible that it is acting simultaneously with the 1,2-DCB, stimulating the pump due to increased membrane permeability and absence of gradient [13, 56, 57]. This could explain why activation and inhibition are more pronounced when measured with the Ca2+-ionophore than when measured with K-oxalate, where the vesicle interior is not gradient-free. We also found that the Ca2+/Pi ratio was partially uncoupled at 1,2-DCB concentrations between 0.05 and 0.5 mM, and fully uncoupled at 1 mM. This phenomenon of uncoupled Ca2+-ATPase with a lower Ca2+/Pi ratio has been previously described in terms of the increased thermogenic activity that occurs when using intact vesicles [13–15], where it was found that after Ca2+ saturation, ~82 % of the hydrolytic activity is uncoupled from the Ca2+ transport, thus decreasing the Ca2+/Pi ratio.
Previous reports showed that non-polar organic solvents facilitate the incorporation of hydrophobic compounds to cell membranes, and that enzymatic stimulation or inhibition is related to both solvent and membrane protein quantity [38, 49]. In agreement with the literature, 1,2-DCB affects SERCA activity by sharing these features, as we further demonstrated that the inhibitory effect of 1,2-DCB was SR membrane-dependent (Fig. 4). This could be due to a partitioning effect of the DCB into the membrane fraction, which indicates that activation or inhibition is dependent on membrane composition and protein concentration. Organic solvents can reduce the inhibition of SERCA by certain drugs, which explains how hydrophobic drugs can move to a lipid environment in the membrane, thus causing enzyme inhibition [49, 50, 58]. Data from Lax et al.  showed that the addition of phosphatidylcholine liposomes to SERCA reactions caused a decrease in the inhibitory effect of the fungicide miconazol. 1,2-DCB shares similar features to those of miconazole and therefore, we believe that our data indicates that DCB is going into the SR vesicles because of its hydrophobic properties.
The Ca2+ uptake assay employed in this study measured accumulated Ca2+ inside the SR vesicles [48, 60]. However, if 1,2-DCB disrupts the time required to fill the vesicles with Ca2+, this may be reflected as an inhibition on the accumulated Ca2+ due to a loss of Ca2+ caused by vesicle damage. Molecules such as 1,2-DCB can target SR membranes affecting the filling time of SR vesicles by the SERCA pump. For example, hexachlorocyclohexane, a hydrophobic compound, was shown to affect the activity of membrane proteins by disrupting the lipid bilayer . For this reason, we filled out SR vesicles with Ca2+ using the active transport of SERCA, followed by the addition of a high concentration of DCB (1 mM) 5 s before stopping the reaction. In agreement with our previous findings, 1,2-DCB was unable to decrease the levels of accumulated Ca2+ when compared to controls (without DCB) (Fig. 5). Given that vesicle filling occurs in less than 1 s, which is the physiological time during which muscle relaxation occurs , we selected a longer vesicle filling time. This data clearly demonstrates that 1,2-DCB is not affecting the vesicle filling time by causing disturbances of the SR membrane. This was supported by our a positive control using Ca2+ ionophore A23187 (absence of gradient) alone or with 1 mM 1,2-DCB, which did not allow accumulation of Ca2+ inside the SR vesicles (Fig. 5). This phenomenon is referred to in the literature as “leaky vesicles”, since this system allows the efflux of luminal Ca2+, thus obtaining faster efflux [61, 62]. It was interesting to find that the SR membrane was not disrupted, which supports our findings that 1,2-DCB is causing uncoupling of the Ca2+/Pi ratio. Considering this phenomenon applied to a biological system, we can refer to the thermogenic activity previously proposed by Inesi and Tadini-Buoninsegni , where SERCA1 uncoupling interferes with the decrease of cytosolic Ca2+ and the subsequent relaxation of muscle fibers. If we take into account that vesicles are filled with Ca2+ in less than 1 s, a sufficient concentration of luminal Ca2+ would not be reached, thus preventing the dissociation of the E2-P.Ca2 form. This phenomenon could not lead to thermogenic activity due to a basal state of the pump. The Ca2+ leakage from the pump could occur if the luminal Ca2+ concentration reached such high levels that both its E2-P.Ca2 dissociation constant and the cytosolic Ca2+ concentrations would remain above the required levels to achieve maximal pump activation, which could occur under prolonged muscle activity. Even though the cytosolic Ca2+ levels can remain high due to multiple action potentials, this does not appear to be our case, since we have no evidence of pump overload (Figs. 2a and 5).
In order to get more insights into the mechanism by which 1,2-DCB caused uncoupling of the SERCA activity, we studied the E1P step of the enzyme. Our results demonstrated that 1,2-DCB decreased the EP stage formation but was influenced by temperature when starting from E2ATP, even when our previous experiments showed that 1,2-DCB produced a high rate of ATP hydrolysis. Our initial expectation was to observe elevated levels of EP by ATP, since it is common that hydrophobic molecules affect the E2 state of SERCA which prevents or decreases the transition to the E1 stage [25, 63]. Our data of EP inhibition suggests that 1,2-DCB (Fig. 6) could be affecting the E2 step. Similar examples of this phenomenon are found in the literature [15, 49, 51, 64, 65]. Taken together, the inhibition of the EP stage and our previous results could explain why an uncoupled Ca2+/Pi ratio was observed, even when Ca2+ is binding to the enzyme in the presence of 1,2-DCB. Phosphorylation of the enzyme is necessary for the appropriate Ca2+ transport across the SR membrane. Therefore, it is possible that a Ca2+ slippage process is taking place, where ATP hydrolysis is occurring without the subsequent accumulation of the transient E1P form and without Ca2+ transport, with Ca2+ “slipping” into the outside of the vesicles. This can be affected by several factors such as time, temperature, enzymatic stage and in our case the DCB concentration [66, 67] Our data shows that at 25 °C, EP levels were increased (Fig. 6b). However, the measurements followed the same pattern of EP decrease with increasing DCB concentration. Regarding the EP measurements conducted at 0 °C, which seem to go against the literature, it is well established that high levels of EP are obtained at low temperatures (0 °C) due to the rapid transition of the limiting E1P.Ca2 step to E2P.Ca2, and the subsequent Ca2+ translocation that leads to the EP decomposition , in other words, the phosphoenzyme formed from ATP is more sensitive to ADP at 0 °C than at 30 °C, thus confirming that ATP hydrolysis coupled to Ca2+ efflux and the ATP↔Pi exchange reaction are inhibited at 0 °C . However, the experiments starting from E2ATP showed higher levels of accumulated EP, but these experiments were conducted at 0 and 25 °C, where A23187 is also present (although protein concentration, time and ATP are different). At 0.05 mM DCB, ~30–40 % of accumulated EP can be observed at both temperatures, indicating that ATP could be protecting the enzyme from inhibition. However, higher DCB concentrations (0.1 mM and subsequent) caused a drastic decrease in EP levels (Fig. 6b, filled circles). Other factors that could affect the E1P to E2P transition include detergents and the microviscosity of the lipid environment of the ATPase . Temperature is another important factor that could potentially affect this transition, as it has been demonstrated that at 21 °C the E1P to E2P isomerization is rapidly affected at high KCl concentrations . Temperature is also important for compound solubility in aqueous solutions, as it has been shown that the solubility of aroma compounds (such as methyl ketones, ethyl esters, aldehyde and alcohol) decreases when incubated on ice as the concentration of the compound is increased, which could contribute to explain the effect of temperature and DCB solubility at 0 and 25 °C in the EP measurements .
Our data demonstrates that 1,2-DCB affects the ATP hydrolytic activity of SERCA, causing both stimulation and inhibition of ATP hydrolysis. This dual effect caused by 1,2-DCB fits it into a common pattern displayed by several other hydrophobic compounds. We also found that 1,2-DCB partially inhibited Ca2+ transport and EP stage formation due to uncoupled enzyme activity, even without affecting the Ca2+ bound to the enzyme. Finally, to the best of our knowledge, this is the first study designed to study the effect of 1,2-DCB, a chemical that is widely found in the environment, on the functionality of SERCA proteins, and thus the data presented here not only provide insights into the molecular mechanisms of the enzyme’s catalytic cycle, but on the possible toxic effects caused by 1,2-DCB.
This project was supported by grant PIFI for CA-02 Diagnosis Academic Group from the Universidad Autónoma de Ciudad Juárez (UACJ), Chihuahua, México. Our recognition of the support provided by Dr. Eppie Rael through NIH grants S06 GM08012 and G12 RR08124 from the University of Texas at El Paso, TX, USA. We also wish to acknowledge the recommendations and comments made by Dr. Armando Gómez-Puyou and Dr. Marietta Tuena de Gómez from the Instituto de Fisiología Celular, UNAM, México.
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