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Fig. 1 | BMC Biochemistry

Fig. 1

From: 1,2-Dichlorobenzene affects the formation of the phosphoenzyme stage during the catalytic cycle of the Ca2+-ATPase from sarcoplasmic reticulum

Fig. 1

Effect of 1,2-DCB and benzene on the initial rates of ATP hydrolysis supported by A23187. The reaction was performed in a buffer containing 20 mM Mops, pH 7.0, 80 mM KCl, 5 mM MgCl2, 1 mM EGTA, 0.967 mM CaCl2, and 1.5 μM A23187. The reaction was conducted by adding 0.01 mg/ml of SR vesicles with different concentrations of 1,2-DCB (mM) or benzene (mM). First data point corresponds to Ca2+-ATPase activity without DCB (control) and it was ~2.6 ± 0.05 μmol Pi/mg∙min

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BMC Biochemistry

ISSN: 1471-2091

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