Hundreds of extracellular proteins polymerise into filaments and matrices by using zona pellucida (ZP) domains. ZP domain proteins perform highly diverse functions, ranging from structural to receptorial, and mutations in their genes are responsible for a number of severe human diseases. Recently, PLAC1, Oosp1-3, Papillote and CG16798 proteins were identified that share sequence homology with the N-terminal half of the ZP domain (ZP-N), but not with its C-terminal half (ZP-C). The functional significance of this partial conservation is unknown.
Results
By exploiting a highly engineered bacterial strain, we expressed in soluble form the PLAC1-homology region of mammalian sperm receptor ZP3 as a fusion to maltose binding protein. Mass spectrometry showed that the 4 conserved Cys residues within the ZP-N moiety of the fusion protein adopt the same disulfide bond connectivity as in full-length native ZP3, indicating that it is correctly folded, and electron microscopy and biochemical analyses revealed that it assembles into filaments.
Conclusion
These findings provide a function for PLAC1-like proteins and, by showing that ZP-N is a biologically active folding unit, prompt a re-evaluation of the architecture of the ZP domain and its polymers. Furthermore, they suggest that ZP-C might play a regulatory role in the assembly of ZP domain protein complexes.
Background
The ZP domain is a sequence of ~260 amino acids that drives polymerisation of a large number of essential secreted proteins from multicellular eukaryotes [1–3]. It has been suggested that the domain, which includes 8 highly conserved Cys residues, consists of two subdomains [4–6]. The N-terminal subdomain (ZP-N) is thought to contain conserved Cys 1 to 4, disulfide-bonded with invariant 1–4, 2–3 connectivity. On the other hand, conserved Cys 5 to 8, located within the C-terminal subdomain (ZP-C), apparently adopt two alternative connectivities in different ZP domain proteins [3, 6–10]. In type I ZP domain proteins with 8 Cys within the ZP domain, such as ZP3, the ZP-C connectivity is 5–7, 6–8; in type II ZP domain proteins with 10 Cys within the ZP domain, like the other egg coat subunits ZP1 and ZP2, it is 5–6, 7-a, b-8 (a and b being the two additional Cys, compared to type I proteins). Interestingly, type I (ZP3-like) ZP domain proteins appear to polymerise into filaments only in the presence of type II (ZP1/ZP2-like) ZP domain proteins, whereas the latter can also form homopolymers.
Recently, placenta protein PLAC1 was described that bears significant homology to the N-terminal subdomain of sperm receptor ZP3 [11, 12]. Based on this similarity, as well as on the observation that deletion of the X chromosome region harboring the PLAC1 gene causes fetal growth restriction and abnormal placenta development [13, 14], it was proposed that PLAC1 might be required for interaction between the trophoblast and other placental or maternal tissues [11, 15]. Five additional proteins, mammalian Oosp1-3 and Drosophila Papillote and CG16798, were subsequently identified that also share homology with ZP-N, but not ZP-C [16–19]. In view of the higher structural conservation of ZP-N, these reports raise questions about the relative contribution of the two subdomains to ZP domain function. Are PLAC1-like proteins also able to polymerise, or do ZP-N sequences carry out a different role than complete ZP domains?
Results
Identification of additional protein sequences containing only ZP-N
To investigate whether other proteins exist that contain only the N-terminal half of the ZP domain, we generated a profile hidden Markov model (HMM) of ZP-N to scan genomic and non-redundant sequence databases. This analysis identified three additional putative ZP-N-containing proteins, whose genes appear to be expressed (Table 1 and Fig. 1, underlined sequences). On the other hand, no proteins containing only ZP-C were found in a parallel search with a corresponding HMM profile. These observations suggest that, unlike ZP-N, ZP-C can be found exclusively within the context of a complete ZP domain.
* E-value; bit score; matched aa (number of Cys). Calibrated expectation values are relative to the NCBI non-redundant protein database (2308679 sequences at the time of the search).
†SignalP probability (aa); SigCleave score (aa).
§This protein is referred to as ZP2 in ref. 41. However, because it contains a trefoil domain immediately before the ZP domain, it should be regarded as a member of the ZP1 family.
Figure 1
Architecture of ZP-N-containing proteins. The primary sequence of each protein is shown as a grey bar, drawn to scale and with the amino and carboxy termini marked. Signal peptides (as identified by SignalP) and transmembrane domains (as predicted by SMART) are represented by red and blue rectangles, respectively; ZP-N sequences are shown as pink rectangles and a trefoil (P) domain is depicted as a yellow rhombus. Proteins are in the same order as in Table 1 and identified by their accession number.
Expression, purification and characterisation of recombinant ZP-N
To establish whether ZP-N is able to fold independently and investigate its biological role, we over-produced in recombinant form the PLAC1-homology region of the ZP domain of mouse ZP3. The 102-amino acid ZP-N fragment was expressed as an affinity sandwich [20], with E. coli maltose binding protein (MBP) fused to its N-terminus via a short linker and a polyhistidine tag (6his) fused to its C-terminus (Fig. 2A). MBP was chosen as a fusion partner since it is strictly monomeric in the presence of maltose [21, 22] and has either no or minimal interaction with the proteins to which it is fused, so that the stoichiometry of MBP fusion proteins is entirely determined by the properties of the non-MBP moieties [22, 23].
Figure 2
Characterisation of MBP-ZP-N-6his. (A) Schematic representation of MBP-ZP-N-6his fusion protein. (B) Multimerisation of MBP-ZP-N-6his. Purified protein, separated by SDS-PAGE under both reducing (R, lanes 2, 4, 6 and 7) and non-reducing (NR, lanes 3 and 5) conditions, was visualised by Coomassie staining (lanes 2, 3) and by immunoblot analysis with monoclonal anti-6his (lanes 4–7). Lane 1, Mr markers; lanes 6 and 7, progressively long exposures of lane 4. The position of bands corresponding to monomeric, dimeric and tetrameric MBP-ZP-N-6his is indicated. (C, D) Disulfide linkages of monomeric MBP-ZP-N-6his. The fusion protein contains 4 Cys residues, all within the ZP-N sequence. Native 1–4, 2–3 disulfides were assigned on the basis of MALDI-TOF-MS measurements of trypsin-digested MBP-ZP-N-6his, performed under non-reducing (NR, C) and reducing (R, D) conditions (Methods). MBP and ZP3 amino acid numbers refer to database entries 1HSJ_A and P10761, respectively. Peaks represent average mass/charge ratio (m/z). Disulfide-bonded and free Cys-residue containing peptides are marked by blue and red circles, respectively; LEH6 C-terminal tag peptide is marked by a black circle; peaks with intensity below 5% are indicated by dashed circles.
Using a bacterial strain that facilitates formation of disulfides by carrying trxB and gor mutations [24] and co-expressing modified versions of disulfide isomerase [24] and thioredoxin [25], significant amounts of MBP-ZP-N-6his were obtained that could be purified to homogeneity with a two-step affinity method (Fig. 2B, lane 2).
Although the fusion protein was soluble, as judged by ultracentrifugation at 100,000 g, it eluted in the void volume of 300 kDa molecular weight (Mr) cut-off size-exclusion columns, suggesting the presence of multimers. Analysis in the presence of ethylenedinitrilotetraacetic acid (EDTA) yielded identical elution profiles, excluding the possibility that trace amounts of Ni2+ ions could have leaked from the immobilised metal ion affinity chromatography (IMAC) column used during purification and caused non-specific protein aggregation by cross-linking multiple histidine tags.
Western blot analysis of purified MBP-ZP-N-6his revealed a band corresponding to monomeric protein and, in addition, a ladder of bands corresponding to dimers, tetramers etc. (i.e. 2n × Mr, with n = 1, 2, ...) (Fig. 2B). Although these multimers were much less abundant under reducing conditions, several lines of evidence suggest that this was due to more extensive denaturation of the ZP domain moiety of MBP-ZP-N-6his, rather than to the presence of spurious intermolecular disulfides. First, unlike the situation reported for other proteins [26], no bands were observed for trimeric, pentameric, etc. (i.e. (2n+1) × Mr) forms of MBP-ZP-N-6his (Fig. 2B). Second, as seen in the case of bands corresponding to the monomeric protein, dimeric and tetrameric MBP-ZP-N-6his also migrated differently under reducing and non-reducing conditions (Fig. 2B, compare lanes 2 and 3, and lanes 5 and 6, 7). Third, when samples were analysed by gel filtration under reducing conditions, most of the protein was still eluted in the void volume. Fourth, mass spectrometric analysis of proteolytic digests of dimeric MBP-ZP-N-6his did not reveal additional peaks compared to monomeric protein, whose spectra were consistent with native, intramolecular disulfides (ZP3 Cys 1 (aa 46)-Cys 4 (aa 139) and Cys 2 (aa 78)-Cys 3 (aa 98)) (Fig. 2C, D) [3, 6–10].
Structural analysis of recombinant ZP-N
Electron microscopy (EM) of negatively stained MBP-ZP-N-6his revealed that the protein assembles into long filaments (Fig. 3A) whose features are reminiscent of the helical structure described for full-length ZP domain proteins (Fig. 3B, C) [2, 3]. Moreover, a pattern was observed in immunolocalisation studies which suggests that dimeric MBP-ZP-N-6his is present as repeating units within filaments (Fig. 3D, E).
Figure 3
MBP-ZP-N-6his assembles into filaments. (A-C) Electron micrographs showing overview (A) and details (B, C) of negatively stained samples. (D, E) Immunogold localisation using monoclonal anti-MBP. Arrows mark closely spaced pairs of beads. Bars represent 0.1 μm.
Discussion
Our results indicate that E. coli-expressed MBP-ZP-N-6his is correctly folded and, because MBP is monomeric and does not influence the multimerisation state of passenger proteins [21–23], that the fusion protein assembles into filaments through its ZP-N sequence. The solubility of purified MBP-ZP-N-6his filaments can be explained by the well documented solubilisation properties of MBP [27, 28]. Furthermore, the periodicity observed by both SDS-PAGE (Fig. 2B) and EM (Fig. 3E) suggests that multimerisation of MBP-ZP-N-6his involves formation of non-covalently linked homodimers. Consistent with these conclusions, a large portion of ZP-C sequence is apparently missing from polymeric Tamm-Horsfall protein due to proteolytic processing between conserved Cys 6 and 7 of the ZP domain [29]. Moreover, homodimerisation of full-length ZP domain proteins, including mammalian ZP3, has been described [3, 9, 30–33].
By demonstrating that ZP-N is a conserved, autonomously folding unit that is biologically active, we suggest that this sequence should be considered a domain on its own and that the current definition of ZP domain should be revised. PLAC1-like proteins are able to polymerise and this explains why the majority of ZP domain mutations causing disease in humans, such as those in α-tectorin and Tamm-Horsfall protein, are clustered within the first half of the domain [3, 34–36]. The importance of ZP-N is also underscored by the observation that ZP domain protein endoglin contains a canonical ZP-N sequence whereas only 2 Cys are conserved within its ZP-C subdomain ([37–40]; accession number AAT84715), and that some fish ZP1 protein isoforms completely lack ZP-C ([41]; Table 1). The availability of a recombinant ZP-N construct able to assemble into filaments that can be easily purified will be instrumental in understanding the effects of these mutations at the molecular level. Our results also raise important questions about the structure of ZP domain filaments and the function of ZP-C. Because the latter is only found as part of a complete ZP domain and can adopt different disulfide connectivities [3, 6–9], it may play a crucial role in regulating the specificity of ZP-N to determine whether or not a given ZP domain protein can homo- or heteropolymerise. Indeed, presence of ZP-C, as well as of hydrophobic patches that regulate polymerisation of ZP domain proteins [4], within full-length ZP3 could explain why – unlike its ZP-N fragment – this is apparently not able to assemble into filaments in the absence of a type II ZP domain counterpart [9, 42, 43]. Alternatively, it is possible that full-length ZP3 and ZP2 are in principle also able to homopolymerise, but the resulting filaments are not stable unless they interact with each other [10].
Conclusion
Recent studies led to the hypothesis that the ZP domain, a module responsible for the polymerisation of a large number of extracellular proteins, consists of two subdomains. In this work, we identified protein sequences sharing homology exclusively with the N-terminal half of the ZP domain (ZP-N), but did not find sequences containing only its C-terminal half (ZP-C). We then showed that a recombinant protein corresponding to the ZP-N region of mammalian sperm receptor ZP3 is able to fold independently from its ZP-C counterpart, and that it assembles into filaments which appear to consist of dimeric subunits. Our results argue that ZP-N should be considered a domain of its own, suggest a function for proteins containing only ZP-N, are consistent with the higher structural conservation of the N-terminal part of the ZP domain, and provide an explanation for the clustering of mutations within ZP-N. Finally, we propose that ZP-C might function by regulating ZP-N-mediated polymerisation of proteins containing a full ZP domain.
Methods
Sequence analysis
Calibrated profile HMMs for ZP-N and ZP-C were generated with HMMER 2.3.2 [44], using sequence databases derived from the Pfam [45] ZP domain protein family (PF00100) alignment. Sequences that were not complete within the amino acid range of interest were removed prior to HMM building. In the case of ZP-N, sequences that did not contain all conserved Cys 1–4 were also excluded, whereas conservation of Cys 5–8 was not explicitly imposed for inclusion of the more divergent ZP-C sequences. Profile HMMs were used to scan Ensembl [46] genome databases and the NCBI Entrez non-redundant protein database (~3800000 total sequences), and matching sequences were automatically extracted and submitted to BLAST [47], CD-SEARCH [48] and SMART [49]. Entries that were either partial (based on the alignment and annotation of matching BLAST sequences) or contained a complete ZP domain (as indicated by CD-SEARCH and/or SMART, as well as by their presence within both ZP-N and ZP-C matches) were filtered out, and remaining entries (~800 sequences) were individually analysed. Final acceptance criteria were high significance and completeness of the matches, as indicated by HMM E-values < 0.1 and extent of the alignment to HMM profiles (together with presence of conserved Cys 1–4 (ZP-N) or Cys 5–8 (ZP-C)), respectively. In addition, since both proteins with a complete ZP domain and PLAC1-like proteins are secreted, matches were accepted only if they also included a putative signal peptide (as predicted by SignalP [50] and EMBOSS SigCleave [51, 52]) which did not overlap with ZP domain sequence (as identified by CD-SEARCH and/or SMART). This analysis yielded 8 unique sequences containing only ZP-N, and no sequences containing only ZP-C (Table 1). An additional mouse sequence with E-value = 1.2 (protein LOC225923; accession number NP_001028455.1) was added to the ZP-N protein list on the basis of its significant similarity to proteins Oosp1 and LOC219990. BLAST and BLAT [53] searches of the mouse genome indicated that the genes encoding proteins Oosp1 and LOC225923, as well as the gene for a third protein (LOC225922; accession number NP_001032723.1) homologous to human LOC219990, are closely located on chromosome 19. The same cluster was independently identified in a recent study, in which LOC225922 and LOC225923 were renamed Oosp2 and Oosp3, respectively [19].
DNA constructs
A PCR fragment encoding aa 42–143 of mouse ZP3 protein was cloned between the Eco R1 and Xho 1 sites of vector pMBP4c, a derivative of plasmid pMBPL-/gp21(338–425) [54] that expresses a C-terminally histidine-tagged modified version of MBP under the control of T7 promoter/lac operator. A second vector, pLJDIS1, was generated from plasmids pBADΔSSdsbC [24] and pFÅ5 [25] to allow co-expression of a version of disulfide isomerase lacking a signal sequence (ΔSSdsbC) and a glutaredoxin-like thioredoxin variant with higher redox potential (TrxA(G33P, P34Y)), under the control of the arabinose promoter. All constructs were verified by DNA sequencing.
Protein expression and purification
For over-expression of MBP-ZP-N-6his, pMBP4c-mZP3(42–143) and pLJDIS1 were co-transformed into E. coli Origami B (DE3) (Novagen), carrying trxB and gor mutations. Although the trxB gor background was crucial to get partially soluble MBP-ZP-N-6his (the protein was completely insoluble in BL21 (DE3)), no significant improvement in solubility was observed upon co-expression of ΔSSdsbC or TrxA(G33P, P34Y). Nevertheless, we decided to still co-express both proteins, because they could be qualitatively important, as they were shown to significantly increase the activity of recombinant disulfide-rich proteins expressed in the cytoplasm of E. coli trxB gor strains [24]. Transformed cells were grown at 37°C in M9 medium containing 0.4% glucose, 15 μg/ml kanamycin, 12.5 μg/ml tetracyclin, 25 μg/ml chloramphenicol and 100 μg/ml carbenicillin. After reaching an optical density (OD595 nm) of 0.5, they were shifted to 24°C for 30 min and pre-induced with 0.2% arabinose. 1 hr 30 min later, cells were induced with 0.1 mM isopropyl-β-D-thiogalactopyranoside and grown for an additional 25 hr at 24°C (final OD595 nm~0.75). Bacteria were harvested by centrifugation and lysed with CelLytic B (Sigma). Soluble MBP-ZP-N-6his was purified by affinity chromatography, using Ni2+-charged HiTrap Chelating HP (Amersham Biosciences) and amylose resin (New England Biolabs) columns, followed by step-gradient ion exchange chromatography, using a Mono Q column (Amersham Biosciences). After dialysis against buffer F (10 mM Na-HEPES pH 8.0, 100 mM NaCl, 1 mM maltose, 1 mM NaN3), the purified protein was concentrated to 16 mg/ml.
Western blotting
Immunoblot experiments were carried out by using BSA-free Penta•His monoclonal primary antibody (1:1000; QIAGEN) and goat anti-mouse horseradish peroxidase (HRP)-conjugated IgG (1:3000; ICN/Cappel), according to the manufacturers protocol. Chemiluminescent detection reactions were performed with Western Lightning Chemiluminescence Reagent Plus (Perkin Elmer).
Mass spectrometry
After SDS-PAGE under non-reducing conditions (with ~20 μg MBP-ZP-N-6his/lane), gel spots were excised and alkylated with 30 mM iodoacetamide in 100 mM Tris-HCl pH 6.8 for 30 min at room temperature. The liquid was removed and samples were prepared for digestion by washing twice with 100 ml 50 mM Tris-HCl pH 6.8/30% acetonitrile (ACN) for 20 min with shaking, then with 100% ACN for 1–2 min. After removing the washes, gel pieces were dried for 30 min in a Speed-Vac concentrator. Individual gel pieces were digested by adding 80 μg modified trypsin or chymotrypsin (sequencing grade, Roche Molecular Biochemicals) in 13–15 ml 25 mM Tris-HCl pH 6.8 and leaving overnight at room temperature. Peptides were extracted with 2 × 50 ml 50% ACN/2% trifluoroacetic acid (TFA) and the combined extracts were divided in half, then dried. One half of the digest was dissolved in matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF-MS) matrix for immediate mass spectrometric analysis, and the other half was reduced by adding 20 mM dithiothreitol (DTT) in 100 mM Tris-HCl pH 8.5. After 30 min at 50°C, the reduced digest was cooled to room temperature and desalted with a C18 ZipTip (Millipore), using 50% ACN to elute the peptides. The eluate was dried and dissolved in MALDI-TOF-MS matrix for analysis. Matrix solution was prepared by making a 10 mg/ml solution of 4-hydroxy-α-cyanocinnamic acid in 50% ACN/0.1% TFA. The dried digest was dissolved in 3 ml matrix solution and 0.7 ml was spotted onto the sample plate. If the sample was not previously desalted, the dried spot was washed twice with water. MALDI mass spectrometric analysis was performed on the digest using a Voyager DE-Pro mass spectrometer (Applied Biosystems) in the linear mode. Spectra were analysed both manually and with MS-Screener [55] and MS-Compare (LJ, unpublished). Since all samples were alkylated prior to digestion, unmodified free Cys-containing peptides identified under non-reducing conditions (Fig. 2C) resulted from laser-induced breakage of disulfides. Furthermore, it appeared that essentially all Cys residues of purified MBP-ZP-N-6his were involved in disulfides. Unlike the case of the Cys 2-Cys 3 disulfide bridge (Fig. 2C), a peak corresponding to a linkage between peptides containing Cys 1 and Cys 4 could not be identified under non-reducing conditions; however, existence of the latter bridge could be clearly inferred by appearance (or marked increase in the intensity) of peaks corresponding to peptides containing unmodified free Cys 1 and Cys 4 upon reduction of the sample (compare Fig. 2C and 2D). This was further supported by a corresponding increase in the intensity of a peak corresponding to the C-terminal tag, which closely follows Cys 4 in the sequence of MBP-ZP-N-6his (Fig. 2C, D). MALDI-TOF-MS analyses of chymotrypsin-digested monomeric protein as well as trypsin-digested dimeric MBP-ZP-N-6his were also consistent with intramolecular 1–4, 2–3 disulfides.
Size-exclusion chromatography
Gel filtration experiments were performed on both FPLC and HPLC systems, using a HiPrep 16/60 Sephacryl S-300 HR column (~300 kDa Mr cut-off; Amersham Biosciences) and a Bio-Sil SEC-250-5 column (~300 kDa Mr cut-off; Bio-Rad), respectively. Running solutions were buffer F (non-reducing conditions) or buffer F + 10 mM DTT (reducing conditions). Additional runs were performed by pre-incubating purified MBP-ZP-N-6his with 10 mM EDTA pH 8.0 for 1 hr at 4°C, before analysis using 10 mM Na-HEPES pH 8.0, 1 mM EDTA as running buffer.
Electron microscopy
For morphological observation, material was negatively stained by applying a drop of solution (final concentration 1 mg/ml) directly onto a 300-mesh formvar-carbon coated nickel grid (Electron Microscopy Sciences), which was allowed to remain for approximately 30 seconds, after which excess solution was removed. A drop of 1% aqueous uranyl acetate was then added onto the grid and allowed to remain for an additional 30 seconds, after which excess solution was removed and the grids allowed to dry. For immunogold localisation, equal volumes of protein (1 mg/ml) and anti-MBP monoclonal primary antibody (1:300; New England Biolabs) diluted in Tris-buffered saline-Tween-20 solution (TBS-T) were allowed to incubate for two hours at room temperature. Goat anti-mouse H&L(Fab2') 10 nm gold-conjugated secondary antibody (1:30/TBS-T, EMS) was added directly to the solution and allowed to incubate for two hours at room temperature. A 300-mesh formvar-carbon coated nickel grid was then immersed and allowed to remain for approximately 30 seconds, after which it was removed and excess solution was removed. Negative contrast staining followed the above-described method. Material was imaged on a Jeol 1200EX electron microscope equipped with an Advanced Imaging Technologies digital camera. Images were imported into Photoshop CS2 (Adobe Systems Inc.) where they were sized and optimised for contrast and brightness.
Abbreviations
6his:
6-histidine tag
aa:
amino acid(s)
ACN:
acetonitrile
DTT:
dithiothreitol
EDTA:
ethylenedinitrilotetraacetic acid
EM:
electron microscopy
FPLC:
fast protein liquid chromatography
HMM:
hidden Markov model
HPLC:
high performance liquid chromatography
IMAC:
immobilised metal ion affinity chromatography
m/z
:
mass/charge ratio
Mr:
molecular weight
MALDI-TOF-MS:
matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry
MBP:
maltose-binding protein
OD:
optical density
SDS-PAGE:
sodium dodecyl sulfate-polyacrylamide gel electrophoresis
TBS-T:
Tris-buffered saline-Tween-20 solution
TFA:
trifluoroacetic acid
ZP:
zona pellucida
Declarations
Acknowledgements
We thank Costel Darie, Mary Ann Gawinowicz and Yelena Milgrom for helpful discussions and comments, Kevin Kelliher and Roman Osman for access to the Mount Sinai School of Medicine bioinformatics cluster, and Frank Schmidt for help with MS-Screener. We are also grateful to Andy Poumbourios for plasmid pMBPL-/gp21(338–425) and to George Georgiou and Jon Beckwith for plasmids pBADΔSSdsbC and pFÅ5. Mass spectrometry analysis was carried out at Columbia University Protein Chemistry Core Facility. This study was supported by National Institutes of Health grant HD35105. LJ was supported in part by a Human Frontier Science Program long-term fellowship.
Authors’ Affiliations
(1)
Brookdale Department of Molecular, Cell and Developmental Biology, Mount Sinai School of Medicine
(2)
Department of Neuroscience, Mount Sinai School of Medicine
(3)
Department of Biosciences and Nutrition, Center for Structural Biochemistry, Karolinska Institutet
References
Bork P, Sander C: A large domain common to sperm receptors (Zp2 and Zp3) and TGF-β type III receptor. FEBS Lett. 1992, 300 (3): 237-240. 10.1016/0014-5793(92)80853-9.PubMedView ArticleGoogle Scholar
Jovine L, Qi H, Williams Z, Litscher E, Wassarman PM: The ZP domain is a conserved module for polymerization of extracellular proteins. Nat Cell Biol. 2002, 4 (6): 457-461. 10.1038/ncb802.PubMedView ArticleGoogle Scholar
Jovine L, Qi H, Williams Z, Litscher ES, Wassarman PM: A duplicated motif controls assembly of zona pellucida domain proteins. Proc Natl Acad Sci USA. 2004, 101 (16): 5922-5927. 10.1073/pnas.0401600101.PubMed CentralPubMedView ArticleGoogle Scholar
Patra AK, Gahlay GK, Reddy BV, Gupta SK, Panda AK: Refolding, structural transition and spermatozoa-binding of recombinant bonnet monkey (Macaca radiata) zona pellucida glycoprotein-C expressed in Escherichia coli. Eur J Biochem. 2000, 267 (24): 7075-7081. 10.1046/j.1432-1327.2000.01808.x.PubMedView ArticleGoogle Scholar
Yonezawa N, Nakano M: Identification of the carboxyl termini of porcine zona pellucida glycoproteins ZPB and ZPC. Biochem Biophys Res Commun. 2003, 307 (4): 877-882. 10.1016/S0006-291X(03)01297-X.PubMedView ArticleGoogle Scholar
Boja ES, Hoodbhoy T, Fales HM, Dean J: Structural characterization of native mouse zona pellucida proteins using mass spectrometry. J Biol Chem. 2003, 278 (36): 34189-34202. 10.1074/jbc.M304026200.PubMedView ArticleGoogle Scholar
Darie CC, Biniossek ML, Jovine L, Litscher ES, Wassarman PM: Structural characterization of fish egg vitelline envelope proteins by mass spectrometry. Biochemistry. 2004, 43 (23): 7459-7478. 10.1021/bi0495937.PubMedView ArticleGoogle Scholar
Zhao M, Boja ES, Hoodbhoy T, Nawrocki J, Kaufman JB, Kresge N, Ghirlando R, Shiloach J, Pannell L, Levine RL, Fales HM, Dean J: Mass spectrometry analysis of recombinant human ZP3 expressed in glycosylation-deficient CHO cells. Biochemistry. 2004, 43 (38): 12090-12104. 10.1021/bi048958k.PubMedView ArticleGoogle Scholar
Monné M, Han L, Jovine L: Tracking down the ZP domain: from the mammalian zona pellucida to the molluscan vitelline envelope. Semin Reprod Med. 2006,Google Scholar
Cocchia M, Huber R, Pantano S, Chen EY, Ma P, Forabosco A, Ko MS, Schlessinger D: PLAC1, an Xq26 gene with placenta-specific expression. Genomics. 2000, 68 (3): 305-312. 10.1006/geno.2000.6302.PubMedView ArticleGoogle Scholar
Hemberger M, Himmelbauer H, Ruschmann J, Zeitz C, Fundele R: cDNA subtraction cloning reveals novel genes whose temporal and spatial expression indicates association with trophoblast invasion. Dev Biol. 2000, 222 (1): 158-169. 10.1006/dbio.2000.9705.PubMedView ArticleGoogle Scholar
Hemberger MC, Pearsall RS, Zechner U, Orth A, Otto S, Ruschendorf F, Fundele R, Elliott R: Genetic dissection of X-linked interspecific hybrid placental dysplasia in congenic mouse strains. Genetics. 1999, 153 (1): 383-390.PubMed CentralPubMedGoogle Scholar
Kushi A, Edamura K, Noguchi M, Akiyama K, Nishi Y, Sasai H: Generation of mutant mice with large chromosomal deletion by use of irradiated ES cells--analysis of large deletion around hprt locus of ES cell. Mamm Genome. 1998, 9 (4): 269-273. 10.1007/s003359900747.PubMedView ArticleGoogle Scholar
Fant M, Weisoly DL, Cocchia M, Huber R, Khan S, Lunt T, Schlessinger D: PLAC1, a trophoblast-specific gene, is expressed throughout pregnancy in the human placenta and modulated by keratinocyte growth factor. Mol Reprod Dev. 2002, 63 (4): 430-436. 10.1002/mrd.10200.PubMedView ArticleGoogle Scholar
Bokel C, Prokop A, Brown NH: Papillote and Piopio: Drosophila ZP-domain proteins required for cell adhesion to the apical extracellular matrix and microtubule organization. J Cell Sci. 2005, 118 (Pt 3): 633-642. 10.1242/jcs.01619.PubMedView ArticleGoogle Scholar
Jazwinska A, Affolter M: A family of genes encoding zona pellucida (ZP) domain proteins is expressed in various epithelial tissues during Drosophila embryogenesis. Gene Expr Patterns. 2004, 4 (4): 413-421. 10.1016/j.modgep.2004.01.003.PubMedView ArticleGoogle Scholar
Yan C, Pendola FL, Jacob R, Lau AL, Eppig JJ, Matzuk MM: Oosp1 encodes a novel mouse oocyte-secreted protein. Genesis. 2001, 31 (3): 105-110. 10.1002/gene.10010.PubMedView ArticleGoogle Scholar
Paillisson A, Dade S, Callebaut I, Bontoux M, Dalbies-Tran R, Vaiman D, Monget P: Identification, characterization and metagenome analysis of oocyte-specific genes organized in clusters in the mouse genome. BMC Genomics. 2005, 6 (1): 76-10.1186/1471-2164-6-76.PubMed CentralPubMedView ArticleGoogle Scholar
Routzahn KM, Waugh DS: Differential effects of supplementary affinity tags on the solubility of MBP fusion proteins. J Struct Funct Genomics. 2002, 2 (2): 83-92. 10.1023/A:1020424023207.PubMedView ArticleGoogle Scholar
Blondel A, Bedouelle H: Export and purification of a cytoplasmic dimeric protein by fusion to the maltose-binding protein of Escherichia coli. Eur J Biochem. 1990, 193 (2): 325-330. 10.1111/j.1432-1033.1990.tb19341.x.PubMedView ArticleGoogle Scholar
Malone JP, Alvares K, Veis A: Structure and assembly of the heterotrimeric and homotrimeric C-propeptides of type I collagen: significance of the α2(I) chain. Biochemistry. 2005, 44 (46): 15269-15279. 10.1021/bi0508338.PubMedView ArticleGoogle Scholar
Smyth DR, Mrozkiewicz MK, McGrath WJ, Listwan P, Kobe B: Crystal structures of fusion proteins with large-affinity tags. Protein Sci. 2003, 12 (7): 1313-1322. 10.1110/ps.0243403.PubMed CentralPubMedView ArticleGoogle Scholar
Bessette PH, Åslund F, Beckwith J, Georgiou G: Efficient folding of proteins with multiple disulfide bonds in the Escherichia coli cytoplasm. Proc Natl Acad Sci USA. 1999, 96 (24): 13703-13708. 10.1073/pnas.96.24.13703.PubMed CentralPubMedView ArticleGoogle Scholar
Mossner E, Huber-Wunderlich M, Rietsch A, Beckwith J, Glockshuber R, Aslund F: Importance of redox potential for the in vivo function of the cytoplasmic disulfide reductant thioredoxin from Escherichia coli. J Biol Chem. 1999, 274 (36): 25254-25259. 10.1074/jbc.274.36.25254.PubMedView ArticleGoogle Scholar
Hellebust H, Bergseth S, Orning L: Expression of the second epidermal growth factor-like domain of human factor VII in Escherichia coli. J Biotechnol. 1998, 66 (2-3): 203-210. 10.1016/S0168-1656(98)00165-5.PubMedView ArticleGoogle Scholar
Fox JD, Routzahn KM, Bucher MH, Waugh DS: Maltodextrin-binding proteins from diverse bacteria and archaea are potent solubility enhancers. FEBS Lett. 2003, 537 (1-3): 53-57. 10.1016/S0014-5793(03)00070-X.PubMedView ArticleGoogle Scholar
Sachdev D, Chirgwin JM: Fusions to maltose-binding protein: control of folding and solubility in protein purification. Methods Enzymol. 2000, 326: 312-321.PubMedView ArticleGoogle Scholar
Fukuoka S, Kobayashi K: Analysis of the C-terminal structure of urinary Tamm-Horsfall protein reveals that the release of the glycosyl phosphatidylinositol-anchored counterpart from the kidney occurs by phenylalanine-specific proteolysis. Biochem Biophys Res Commun. 2001, 289 (5): 1044-1048. 10.1006/bbrc.2001.6112.PubMedView ArticleGoogle Scholar
Hikita C, Vijayakumar S, Takito J, Erdjument-Bromage H, Tempst P, Al-Awqati Q: Induction of terminal differentiation in epithelial cells requires polymerization of hensin by galectin 3. J Cell Biol. 2000, 151 (6): 1235-1246. 10.1083/jcb.151.6.1235.PubMed CentralPubMedView ArticleGoogle Scholar
Paquet ME, Pece-Barbara N, Vera S, Cymerman U, Karabegovic A, Shovlin C, Letarte M: Analysis of several endoglin mutants reveals no endogenous mature or secreted protein capable of interfering with normal endoglin function. Hum Mol Genet. 2001, 10 (13): 1347-1357. 10.1093/hmg/10.13.1347.PubMedView ArticleGoogle Scholar
Sasanami T, Pan J, Doi Y, Hisada M, Kohsaka T, Toriyama M: Secretion of egg envelope protein ZPC after C-terminal proteolytic processing in quail granulosa cells. Eur J Biochem. 2002, 269 (8): 2223-2231. 10.1046/j.1432-1033.2002.02880.x.PubMedView ArticleGoogle Scholar
Takeuchi Y, Cho R, Iwata Y, Nishimura K, Kato T, Aoki N, Kitajima K, Matsuda T: Morphological and biochemical changes of isolated chicken egg-envelope during sperm penetration: degradation of the 97-kilodalton glycoprotein is involved in sperm-driven hole formation on the egg-envelope. Biol Reprod. 2001, 64 (3): 822-830. 10.1095/biolreprod64.3.822.PubMedView ArticleGoogle Scholar
Moreno-Pelayo MA, del Castillo I, Villamar M, Romero L, Hernandez-Calvin FJ, Herraiz C, Barbera R, Navas C, Moreno F: A cysteine substitution in the zona pellucida domain of α-tectorin results in autosomal dominant, postlingual, progressive, mid frequency hearing loss in a Spanish family. J Med Genet. 2001, 38 (5): E13-E16. 10.1136/jmg.38.5.e13.PubMed CentralPubMedView ArticleGoogle Scholar
Tinschert S, Ruf N, Bernascone I, Sacherer K, Lamorte G, Neumayer HH, Nurnberg P, Luft FC, Rampoldi L: Functional consequences of a novel uromodulin mutation in a family with familial juvenile hyperuricaemic nephropathy. Nephrol Dial Transplant. 2004, 19 (12): 3150-3154. 10.1093/ndt/gfh524.PubMedView ArticleGoogle Scholar
Verhoeven K, Van Laer L, Kirschhofer K, Legan PK, Hughes DC, Schatteman I, Verstreken M, Van Hauwe P, Coucke P, Chen A, Smith RJ, Somers T, Offeciers FE, Van de Heyning P, Richardson GP, Wachtler F, Kimberling WJ, Willems PJ, Govaerts PJ, Van Camp G: Mutations in the human α-tectorin gene cause autosomal dominant non-syndromic hearing impairment. Nat Genet. 1998, 19 (1): 60-62.PubMedView ArticleGoogle Scholar
Gougos A, Letarte M: Primary structure of endoglin, an RGD-containing glycoprotein of human endothelial cells. J Biol Chem. 1990, 265 (15): 8361-8364.PubMedGoogle Scholar
Yamashita H, Ichijo H, Grimsby S, Moren A, ten Dijke P, Miyazono K: Endoglin forms a heteromeric complex with the signaling receptors for transforming growth factor-β. J Biol Chem. 1994, 269 (3): 1995-2001.PubMedGoogle Scholar
Ge AZ, Butcher EC: Cloning and expression of a cDNA encoding mouse endoglin, an endothelial cell TGF-β ligand. Gene. 1994, 138 (1-2): 201-206. 10.1016/0378-1119(94)90808-7.PubMedView ArticleGoogle Scholar
Raab U, Lastres P, Arevalo MA, Lopez-Novoa JM, Cabanas C, de la Rosa EJ, Bernabeu C: Endoglin is expressed in the chicken vasculature and is involved in angiogenesis. FEBS Lett. 1999, 459 (2): 249-254. 10.1016/S0014-5793(99)01252-1.PubMedView ArticleGoogle Scholar
Chang YS, Hsu CC, Wang SC, Tsao CC, Huang FL: Molecular cloning, structural analysis, and expression of carp ZP2 gene. Mol Reprod Dev. 1997, 46 (3): 258-267. 10.1002/(SICI)1098-2795(199703)46:3<258::AID-MRD4>3.0.CO;2-O.PubMedView ArticleGoogle Scholar
Rankin T, Talbot P, Lee E, Dean J: Abnormal zonae pellucidae in mice lacking ZP1 result in early embryonic loss. Development. 1999, 126 (17): 3847-3855.PubMedGoogle Scholar
Rankin TL, O'Brien M, Lee E, Wigglesworth K, Eppig J, Dean J: Defective zonae pellucidae in Zp2-null mice disrupt folliculogenesis, fertility and development. Development. 2001, 128 (7): 1119-1126.PubMedGoogle Scholar
Bendtsen JD, Nielsen H, von Heijne G, Brunak S: Improved prediction of signal peptides: SignalP 3.0. J Mol Biol. 2004, 340 (4): 783-795. 10.1016/j.jmb.2004.05.028.PubMedView ArticleGoogle Scholar
von Heijne G: Sequence analysis in molecular biology: treasure trove or trivial pursuit. 1987, London , Academic Press, 113-117.Google Scholar
Rice P, Longden I, Bleasby A: EMBOSS: The European Molecular Biology Open Software Suite. Trends Genet. 2000, 16 (6): 276-277. 10.1016/S0168-9525(00)02024-2.PubMedView ArticleGoogle Scholar
Kent WJ: BLAT - The BLAST-like alignment tool. Genome Res. 2002, 12 (4): 656-664. 10.1101/gr.229202. Article published online before March 2002.PubMed CentralPubMedView ArticleGoogle Scholar
Center RJ, Kobe B, Wilson KA, Teh T, Howlett GJ, Kemp BE, Poumbourios P: Crystallization of a trimeric human T cell leukemia virus type 1 gp21 ectodomain fragment as a chimera with maltose-binding protein. Protein Sci. 1998, 7 (7): 1612-1619.PubMed CentralPubMedView ArticleGoogle Scholar
Thiede B, Hohenwarter W, Krah A, Mattow J, Schmid M, Schmidt F, Jungblut PR: Peptide mass fingerprinting. Methods. 2005, 35 (3): 237-247. 10.1016/j.ymeth.2004.08.015.PubMedView ArticleGoogle Scholar
Reboul J, Vaglio P, Rual JF, Lamesch P, Martinez M, Armstrong CM, Li S, Jacotot L, Bertin N, Janky R, Moore T, Hudson JRJ, Hartley JL, Brasch MA, Vandenhaute J, Boulton S, Endress GA, Jenna S, Chevet E, Papasotiropoulos V, Tolias PP, Ptacek J, Snyder M, Huang R, Chance MR, Lee H, Doucette-Stamm L, Hill DE, Vidal M: C. elegans ORFeome version 1.1: experimental verification of the genome annotation and resource for proteome-scale protein expression. Nat Genet. 2003, 34 (1): 35-41. 10.1038/ng1140.PubMedView ArticleGoogle Scholar
Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K, Kimura K, Makita H, Sekine M, Obayashi M, Nishi T, Shibahara T, Tanaka T, Ishii S, Yamamoto J, Saito K, Kawai Y, Isono Y, Nakamura Y, Nagahari K, Murakami K, Yasuda T, Iwayanagi T, Wagatsuma M, Shiratori A, Sudo H, Hosoiri T, Kaku Y, Kodaira H, Kondo H, Sugawara M, Takahashi M, Kanda K, Yokoi T, Furuya T, Kikkawa E, Omura Y, Abe K, Kamihara K, Katsuta N, Sato K, Tanikawa M, Yamazaki M, Ninomiya K, Ishibashi T, Yamashita H, Murakawa K, Fujimori K, Tanai H, Kimata M, Watanabe M, Hiraoka S, Chiba Y, Ishida S, Ono Y, Takiguchi S, Watanabe S, Yosida M, Hotuta T, Kusano J, Kanehori K, Takahashi-Fujii A, Hara H, Tanase TO, Nomura Y, Togiya S, Komai F, Hara R, Takeuchi K, Arita M, Imose N, Musashino K, Yuuki H, Oshima A, Sasaki N, Aotsuka S, Yoshikawa Y, Matsunawa H, Ichihara T, Shiohata N, Sano S, Moriya S, Momiyama H, Satoh N, Takami S, Terashima Y, Suzuki O, Nakagawa S, Senoh A, Mizoguchi H, Goto Y, Shimizu F, Wakebe H, Hishigaki H, Watanabe T, Sugiyama A, Takemoto M, Kawakami B, Yamazaki M, Watanabe K, Kumagai A, Itakura S, Fukuzumi Y, Fujimori Y, Komiyama M, Tashiro H, Tanigami A, Fujiwara T, Ono T, Yamada K, Fujii Y, Ozaki K, Hirao M, Ohmori Y, Kawabata A, Hikiji T, Kobatake N, Inagaki H, Ikema Y, Okamoto S, Okitani R, Kawakami T, Noguchi S, Itoh T, Shigeta K, Senba T, Matsumura K, Nakajima Y, Mizuno T, Morinaga M, Sasaki M, Togashi T, Oyama M, Hata H, Watanabe M, Komatsu T, Mizushima-Sugano J, Satoh T, Shirai Y, Takahashi Y, Nakagawa K, Okumura K, Nagase T, Nomura N, Kikuchi H, Masuho Y, Yamashita R, Nakai K, Yada T, Nakamura Y, Ohara O, Isogai T, Sugano S: Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 2004, 36 (1): 40-45. 10.1038/ng1285.PubMedView ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
