The roles of aldehyde dehydrogenases (ALDHs) in the PDH bypass of Arabidopsis
© Wei et al; licensee BioMed Central Ltd. 2009
Received: 12 August 2008
Accepted: 25 March 2009
Published: 25 March 2009
Eukaryotic aldehyde dehydrogenases (ALDHs, EC 1.2.1), which oxidize aldehydes into carboxylic acids, have been classified into more than 20 families. In mammals, Family 2 ALDHs detoxify acetaldehyde. It has been hypothesized that plant Family 2 ALDHs oxidize acetaldehyde generated via ethanolic fermentation, producing acetate for acetyl-CoA biosynthesis via acetyl-CoA synthetase (ACS), similar to the yeast pathway termed the "pyruvate dehydrogenase (PDH) bypass". Evidence for this pathway in plants has been obtained from pollen.
To test for the presence of the PDH bypass in the sporophytic tissue of plants, Arabidopsis plants homozygous for mutant alleles of all three Family 2 ALDH genes were fed with 14C-ethanol along with wild type controls. Comparisons of the incorporation rates of 14C-ethanol into fatty acids in mutants and wild type controls provided direct evidence for the presence of the PDH bypass in sporophytic tissue. Among the three Family 2 ALDHs, one of the two mitochondrial ALDHs (ALDH2B4) appears to be the primary contributor to this pathway. Surprisingly, single, double and triple ALDH mutants of Arabidopsis did not exhibit detectable phenotypes, even though a Family 2 ALDH gene is required for normal anther development in maize.
The PDH bypass is active in sporophytic tissue of plants. Blocking this pathway via triple ALDH mutants does not uncover obvious visible phenotypes.
Aldehydes vary in length and in characteristics of their alkyl chains but all are usually deleterious to biological systems due to their chemical reactivity. Aldehyde dehydrogenases (ALDHs, EC 1.2.1) oxidize aldehydes into carboxylic acids, using NAD+ or NADP+ as a co-factor. As such ALDHs play an important role in detoxifying aldehydes that are generated endogenously or introduced from the environment. ALDHs are very diverse in that some only use either NAD+ or NADP+ as the co-factor, while others can use both, some oxidize only a limited number of aldehydes, while others have broader substrate spectra, and ALDHs exist in various subcellular compartments, including the cytosol, mitochondria, plastids and microsomes.
Over 550 ALDH genes have been identified across virtually all species, and those from eukaryotes have been classified into more than 20 families . Family 2 ALDHs are mitochondrial or cytosolic homotetrameric enzymes. The well studied human mitochondrial Family 2 ALDH, ALDH2, detoxifies acetaldehyde generated via alcohol intake . Family 2 ALDHs in plants have gained attention since the cloning of rf2a gene, a nuclear restorer gene for cytoplasm male sterility in maize, which encodes a mitochondrial Family 2 ALDH, RF2A . Although the molecular mechanisms associated with the restorer function of the rf2a gene remain to be resolved, several studies in maize and other species have provided clues as to the physiological functions of Family 2 ALDHs in plants.
Consistent with the physiological function of human Family 2 ALDH in detoxifying acetaldehyde, Liu and Schnable  demonstrated that acetaldehyde is one of the best substrates in vitro for RF2A, based on the ratio of Kcat to Km. In addition, one of the mitochondrial Family 2 ALDHs in rice may be responsible for efficient detoxification of acetaldehyde during re-aeration after submergence of rice plants [5, 6]. These studies all suggest a role of Family 2 ALDHs during ethanolic fermentation, which is catalyzed by pyruvate decarboxylase (PDC) and alcohol dehydrogenase (ADH) and generates acetaldehyde and ethanol. The detoxification of acetaldehyde by ALDH produces acetate. In yeast and mammals, it has been established that acetyl-CoA synthetase (ACS) can utilize acetate to synthesize acetyl-CoA, both in the mitochondria [7, 8] and in the cytosol [9, 10].
The Arabidopsis thaliana ACS is targeted to the plastid and is encoded by a single gene [11–13]. In plastids acetyl-CoA is utilized for de novo fatty acid biosynthesis. Although the acetyl-CoA pool generated by ACS from acetate seems redundant for fatty acid biosynthesis, ACS is hypothesized to play a specialized role in certain cells and tissues . Besides, the redundancy of acetyl-CoA pool from ACS observed above might be due to the low concentration of acetate, probably 0.05 mM (less than one third the Km of this enzyme) [13, 14]. Indeed, the feeding of radio-labeled acetate indicates that isolated plastids can use exogenous acetate for fatty acid synthesis . To understand how and when ACS contributes to the acetyl-CoA pool of plastids, the study of acetate biosynthesis would be the key, which is readily diffusible across membranes. One potential pathway would be through ALDH utilizing acetaldehyde generated via ethanolic fermentation by PDC from pyruvate or by ADH from ethanol.
There are three Family 2 ALDHs in Arabidopsis , one of which, ALDH2C4 (AT3G24503), is localized to the cytosol. One physiological function of ALDH2C4 is the production of ferulic acid and sinapic acid during lignin biosynthesis . The other two ALDHs, ALDH2B4 (AT3G48000) [23, 24] and ALDH2B7 (AT1G23800) , are targeted to mitochondria. Their physiological functions have not yet been described.
Identification of null mutants of the three Family 2 ALDH genes
Gene-specific primers used along with T-DNA left border primera for genotyping
Gene Specific Primers
Direct evidence for the presence of PDH bypass for acetyl-CoA biosynthesis
An ideal experiment to directly test for the presence of the PDH bypass (the PDC-ALDH-ACS pathway) in plants would be to compare the differences between wild type and mutants of involved genes in the incorporation of 14C-pyruvate or 14C-acetaldehyde into acetyl-CoA in plants in which the PDH pathway has been blocked. This is not possible for two reasons. First, blocking the Arabidopsis PDH pathway by mutating the E2 subunit of the PDH complex results in lethality . Second, pyruvate is unstable and acetaldehyde is toxic. Hence, instead 14C-ethanol was fed to plants that were wild type for the PDH pathway and either wild type or mutant for the ALDH genes. The substitution of ethanol for pyruvate or acetaldehyde is appropriate because ethanol should be predominantly, if not exclusively, oxidized to acetaldehyde via ADH (Figure 1). Because well established means to detect acetyl-CoA do not exist, flux through the PDH bypass was measured by determining the incorporation of 14C-ethanol into extracted saponifiable lipids, which should consist primarily of fatty acids .
14C-ethanol was fed to whole seedlings, seedling leaves, and inflorescences. The incorporation rates were compared between single or double aldh mutants and their wild type siblings. No differences were detected in whole seedlings or seedling leaves between the aldh2C4 homozygous mutants from either allele (aldh2C4-1 and aldh2C4-2) and their wild type siblings (data not shown). We, therefore, conclude that the cytosolic Family 2 ALDH is either not involved in the ADH-ALDH-ACS pathway, or can be compensated for by the mitochondrial paralog(s).
ALDH2B4 appears to play a more substantial role in this pathway than does ALDH2B7. This conclusion is based in part on the observation that in both tissues the aldh2B4 single mutant exhibits less incorporation than the wild type. In addition, in both tissues, the rate of incorporation is not statistically different in the double mutant than in the aldh2B4 single mutant. Hence, there is no evidence to indicate that ALDH2B7 contributes substantially to this pathway. Surprisingly, in both tissues, the aldh2B7 single mutant (ALDH2B4;aldh2B7) exhibits more incorporation than the wild type, but only in the inflorescence is this difference statistically significant. This may indicate that in the aldh2B7 mutant, the plant responds by increasing the expression of ALDH2B4, but this hypothesis remains to be tested.
Expression analyses of Family 2 ALDH genes
Gene specific primers used for qRT-PCR
ALDH mutants do not exhibit obvious visible phenotypes
No obvious visible phenotypes, including total seed production, plant size, or flowering time, were observed to be associated with any of the single, double or triple ALDH mutants when grown in soil or on MS media. These genotypes were also subjected to several stress conditions, but again no obvious phenotypic differences were observed between mutant and wild type plants. Tested conditions included oxidative stress (4 × 10-5 M Rose Bengal in MS media), ABA treatment (10-5 M in MS media), acetaldehyde (20 mM in MS media) or ethanol (0.5% in MS media), cold treatment (4°C in the dark for 24 hours), heat treatment (40°C in the dark for 12 hours), hypoxia treatments (plants on MS media plates submerged in water in the dark for 6 hours).
The PDC-ALDH-ACS pathway is termed the PDH bypass in yeast . Studies conducted in the Kuhlemeier lab  demonstrated that ethanol fed to tobacco pollen can be incorporated into fatty acids, which suggested the presence of the PDC-ALDH-ACS pathway for generating acetyl-CoA for de novo fatty acid biosynthesis. Analysis of a PDC mutant of petunia provided further support for the expression of the PDH bypass in pollen . This study left open, however, the question of whether the PDH bypass functions in sporophytic tissue. We used a reverse genetics approach to compare the incorporation rates of 14C-ethanol into fatty acids in sporophytic tissue (seedlings) of Arabidopsis aldh mutants versus their wild type controls. This study and that of Lin and Oliver , which demonstrated lower incorporation rates in an ACS knockout mutant than wild type, provide direct evidence for the presence of the PDH bypass in sporophytic tissue of plants. Specifically, lower rates of incorporation were observed in the aldh2B4;aldh2B7 double mutant as compared to wild type controls, in both whole seedlings and inflorescences.
The aldh2C4 single mutant did not exhibit differences in incorporation relative to wild type. Because ALDH2C4 can oxidize acetaldehyde, at least in vitro , this result suggests either that ALDH2C4 is not required for the PDH bypass or that it is involved in the PDH bypass but its absence can be compensated for by the presence of one or both of the mitochondrial ALDHs. Of the two mitochondrial ALDHs, ALDH2B4 appears to play a more important role in the PDH bypass pathway in both whole seedlings and inflorescences.
This study establishes the presence of the PDH bypass in whole seedlings, thereby demonstrating that the PDH bypass is not exclusive to pollen . This conclusion is consistent with our qRT-PCR results, which show constitutive expression of ALDH2B4. On the other hand, the findings that a knockout of ALDH2B4 has no discernable phenotype and that an ACS knockout mutant has only modest effects on plant growth  suggests that the PDH bypass is not essential under tested conditions. It is not clear whether the PDH bypass is involved in the biosynthesis of acetyl-CoA or the removal of fermentation intermediates. Lin and Oliver  provided evidence for the latter in that their ACS knockout mutant is more sensitive to ethanol, acetaldehyde and acetate than are controls. Surprisingly, there are no obvious visible phenotypes associated with any of the single, double or triple Family 2 ALDH mutants, even though a Family 2 ALDH has an essential role in the development of maize anthers .
The PDH bypass, which has previously been shown to be expressed in pollen, can produce acetyl-CoA in the absence of pyruvate dehydrogenase (PDH). We provide evidence of the existence of the PDH bypass in sporophytic tissue. Surprisingly, no obvious visible phenotypes are associated with the triple ALDH mutants, which should block this pathway.
Growth of plants
Wild type and T-DNA insertion lines of Arabidopsis seeds were planted in soil. After planting, they were allowed to imbibe for 2–4 days at 4°C before transferred to 24 hour light conditions in a growth chamber at 22°C under a light intensity of 110 ± 5 μmol m-2 S-1.
Identification and genotyping of T-DNA knockout lines
aldh2B4-1 (CSJ2971) and aldh2B7-1 (CSJ989) T-DNA knockout lines were identified in association with the Arabidopsis Knockout Facility at the University of Wisconsin following their standard procedures. aldh2C4-1 (SALK_027911), aldh2C4-2 (SALK_024974), and aldh2B4-2 (SALK_078568)T-DNA knockout lines were identified in the Salk Institute T-DNA insertion library database http://signal.salk.edu/cgi-bin/tdnaexpress by BLAST searches.
The T-DNA left border primers JL202 (5'-CATTTTATAATAACGCTGCGGACATCTAC-3') and LBa1 (5'-TGGTTCACGTAGTGGGCCATCG-3') were used in combination with gene specific primers to genotype lines from the University of Wisconsin and from the SALK Institute, respectively (Table 1). LBa1 was used instead of LBb1 (see SALK website) because under our PCR conditions LBb1 can self amplify a band of ~450 bp from Columbia and WS wild type plants.
Generation of double and triple mutants
To obtain double mutants, crosses were made between plants homozygous for different single mutants to generate a plant that was heterozygous for both. Progeny from this plant were then genotyped. The aldh2B4;aldh2B7 double mutant in the uniform WS genetic background carried the aldh2B4-1 and aldh2B7-1 alleles, and that in a mixed Columbia and WS background carried the aldh2B4-2 and aldh2B7-1 alleles. The two types of triple mutants carried the aldh2B4-1, aldh2B7-1 and one of the two aldh2C4 alleles.
Polymerase Chain Reaction (PCR)
Each PCR reaction included 0.2 mM dNTP, 2.0 mM MgCl2, 0.5 μM of each primer, and Taq polymerase in a total volume of 20 μl. PCR reactions were conducted for 32 cycles, with each cycle conducted at 94° for 30 sec, followed by the appropriate annealing temperature for 45 sec, and then extended at 72° for 1 min.
14C feeding and isolation of fatty acids
Per replication, one intact seedling (2 to 4 weeks old) removed from the soil with roots cleaned using a paper towel, one seedling leaf, or the top cluster of flower buds and opened flowers from the main branch of an inflorescence, was weighed, and placed into a 1.5 ml microfuge tube containing 100 μl of carrier (20 mM ethanol) plus 1 μCi of 14C-ethanol (Sigma product # 312975). Tubes were incubated in a growth chamber (22°C; 110 ± 5 μmol m-2 S-1 light intensity) with the lid open. After four hours plant tissues were dried with a paper towel and placed into 1 ml Hexane:Isopropanol (3:2) solution for short-term storage if needed. Fatty acids were isolated using the protocol of . Data were collected from four replications.
Tissue collection, RNA isolation and reverse-transcriptase PCR
Tissues were collected from one month old adult plants growing in soil in the growth chamber as described above, harvested in the following order: green siliques, open flowers, flower buds, cauline leaves, stems, rosette leaves and roots (washed by water and dried with paper towel). After harvest, samples were immediately submerged in liquid nitrogen and stored at -80°C until RNA isolation was performed.
Tissues were ground with a mortar and pestle in liquid nitrogen, and RNA was isolated with a modified "acid guanidinium thiocyanate-phenol-chloroform extraction" method [28, 29] as described in , except for a slightly different recipe for Trizol (38% phenol equilibrated pH 4.3, 1 M guanidine thiocyanate, 1 M ammonium thiocyanate, 0.1 M sodium acetate pH5, 5% glycerol).
First strand cDNA was synthesized with poly dT primer using SuperScript II RNase H Reverse Transcriptase (Cat. No. 18064-014, Invitrogen, CA).
Two microliters of the first strand cDNA were used for PCR to test the transcription of the T-DNA insertion alleles. All primer pairs flank at least one intron.
The following pair of primers was used for aldh2C4-1 and aldh2C4-2: 5'-AACTTCTCCACAACCTTATCGTAT-3' (forward) and 5'-ACGGAGCCACGACGGTGAAGTTAC-3' (reverse).
The following pair of primers was used for aldh2B4-1 and aldh2B4-2: 5'-CTACTGGATGTGCCTGAAGCATC-3' (forward) and 5'-CATGAGTCTTTAGAGAACCCAAAG-3' (reverse).
For aldh2B7-1, the primer sequences are 5'-AGTACCAATGCTTGCTAGGG-3' (forward) and 5'-AGCTTGTAATGTGGCTCCAG-3' (reverse).
The primer sequences used for the positive control actin2 (GenBank accession no. U37281) are the same as used by .
Quantitative Real-Time PCR (qRT-PCR)
Procedures similar to those described in  were used. The criteria for designing primers (Table 2) using Primer 3  were as follows: Tm, 58°C to 61°C, no difference >2°C between the primers in a pair; primer length, 19–24 bp; GC content, 45–55%; amplicon length, 100–200 bp. Only primers yielding a single product in conventional PCR and qRT-PCR were used. qRT-PCR was conducted by using an Mx4000 multiplex quantitative PCR system (Stratagene). A human gene (GenBank accession no. AA418251) was spiked into each reaction as an external reference for data normalization.
qRT-PCR data were initially analyzed by using MX4000 analysis software. Ct values for each target gene and reference gene were calculated by using baseline-corrected, ROX-normalized parameters. Three technical replicates were included in each plate, and the average Ct value for each gene of interest was normalized within a plate to the human reference gene by computing 2^ΔCt (reference – target)  to indicate the relative amount of expression level compared to the reference gene. This was normalized again by the amount difference of starting RNA. The 2^ΔCt (reference – target) values from three biological replicates were used to calculate standard errors.
We thank the Salk Institute Genomic Analysis Laboratory for providing the sequence-indexed Arabidopsis T-DNA insertion lines, the Arabidopsis Knockout Facility at the University of Wisconsin for the screening service of T-DNA insertion lines, and the Arabidopsis Biological Resource Center Stock Center (Ohio State University, Columbus) for providing seeds. We thank former lab members, Dr. Tsui-Jung Wen, Dr. Yongjie Yang, Dr. Marna D. Yandeau-Nelson, and Chi-Hong Kuo, for participating in the initial screening for T-DNA mutants from the University of Wisconsin. We thank Dr. An-Ping Hsia for assisting with the preparation of the manuscript. This research was funded by grants from the USDA (NRI 00-0347 and 02-01419) and the DOE (DE-FG02-01ER15170).
- Sophos NA, Vasiliou V: Aldehyde dehydrogenase gene superfamily: the 2002 update. Chem Biol Interact. 2003, 143–144: 5-22. 10.1016/S0009-2797(02)00163-1.View ArticleGoogle Scholar
- Li SY, Gomelsky M, Duan J, Zhang Z, Gomelsky L, Zhang X, Epstein PN, Ren J: Overexpression of aldehyde dehydrogenase-2 (ALDH2) transgene prevents acetaldehyde-induced cell injury in human umbilical vein endothelial cells: role of ERK and p38 mitogen-activated protein kinase. J Biol Chem. 2004, 279 (12): 11244-11252. 10.1074/jbc.M308011200.View ArticleGoogle Scholar
- Cui X, Wise RP, Schnable PS: The rf2 nuclear restorer gene of male-sterile T-cytoplasm maize. Science. 1996, 272 (5266): 1334-1336. 10.1126/science.272.5266.1334.View ArticleGoogle Scholar
- Liu F, Schnable PS: Functional specialization of maize mitochondrial aldehyde dehydrogenases. Plant Physiol. 2002, 130 (4): 1657-1674. 10.1104/pp.012336.PubMed CentralView ArticleGoogle Scholar
- Nakazono M, Tsuji H, Li Y, Saisho D, Arimura S, Tsutsumi N, Hirai A: Expression of a gene encoding mitochondrial aldehyde dehydrogenase in rice increases under submerged conditions. Plant Physiol. 2000, 124 (2): 587-598. 10.1104/pp.124.2.587.PubMed CentralView ArticleGoogle Scholar
- Tsuji H, Meguro N, Suzuki Y, Tsutsumi N, Hirai A, Nakazono M: Induction of mitochondrial aldehyde dehydrogenase by submergence facilitates oxidation of acetaldehyde during re-aeration in rice. FEBS Lett. 2003, 546 (2–3): 369-373. 10.1016/S0014-5793(03)00631-8.View ArticleGoogle Scholar
- Yamashita H, Fukuura A, Nakamura T, Kaneyuki T, Kimoto M, Hiemori M, Tsuji H: Purification and partial characterization of acetyl-coA synthetase in rat liver mitochondria. J Nutr Sci Vitaminol (Tokyo). 2002, 48 (5): 359-364.View ArticleGoogle Scholar
- Klein HP, Jahnke L: Effects of aeration on formation and localization of the acetyl coenzyme A synthetases of Saccharomyces cerevisiae. J Bacteriol. 1979, 137 (1): 179-184.PubMed CentralGoogle Scholar
- Loikkanen D, Haghighi S, Vainio S, Pajunen A: Expression of cytosolic acetyl-CoA synthetase gene is developmentally regulated. Mechanisms of Development. 2002, 115 (1–2): 139-141. 10.1016/S0925-4773(02)00097-7.View ArticleGoogle Scholar
- Akamatsu S, Kamiya H, Yamashita N, Motoyoshi T, Goto-Yamamoto N, Ishikawa T, Okazaki N, Nishimura A: Effects of aldehyde dehydrogenase and acetyl-CoA synthetase on acetate formation in sake mash. J Biosci Bioeng. 2000, 90 (5): 555-560.View ArticleGoogle Scholar
- Ke J, Behal RH, Back SL, Nikolau BJ, Wurtele ES, Oliver DJ: The role of pyruvate dehydrogenase and acetyl-coenzyme A synthetase in fatty acid synthesis in developing Arabidopsis seeds. Plant Physiol. 2000, 123 (2): 497-508. 10.1104/pp.123.2.497.PubMed CentralView ArticleGoogle Scholar
- Lin M, Behal R, Oliver DJ: Disruption of plE2, the gene for the E2 subunit of the plastid pyruvate dehydrogenase complex, in Arabidopsis causes an early embryo lethal phenotype. Plant Mol Biol. 2003, 52 (4): 865-872. 10.1023/A:1025076805902.View ArticleGoogle Scholar
- Behal RH, Lin M, Back S, Oliver DJ: Role of acetyl-coenzyme A synthetase in leaves of Arabidopsis thaliana. Arch Biochem Biophys. 2002, 402 (2): 259-267. 10.1016/S0003-9861(02)00086-3.View ArticleGoogle Scholar
- Bao X, Focke M, Pollard M, Ohlrogge J: Understanding in vivo carbon precursor supply for fatty acid synthesis in leaf tissue. Plant J. 2000, 22 (1): 39-50. 10.1046/j.1365-313x.2000.00712.x.View ArticleGoogle Scholar
- Kang F, Rawsthorne S: Starch and Fatty-Acid Synthesis in Plastids from Developing Embryos of Oilseed Rape (Brassica-Napus L). Plant Journal. 1994, 6 (6): 795-805. 10.1046/j.1365-313X.1994.6060795.x. [http://www.ncbi.nlm.nih.gov/sites/entrez]View ArticleGoogle Scholar
- Boubekeur S, Camougrand N, Bunoust O, Rigoulet M, Guerin B: Participation of acetaldehyde dehydrogenases in ethanol and pyruvate metabolism of the yeast Saccharomyces cerevisiae. Eur J Biochem. 2001, 268 (19): 5057-5065. 10.1046/j.1432-1033.2001.02418.x.View ArticleGoogle Scholar
- Pronk JT, Wenzel TJ, Luttik MA, Klaassen CC, Scheffers WA, Steensma HY, van Dijken JP: Energetic aspects of glucose metabolism in a pyruvate-dehydrogenase-negative mutant of Saccharomyces cerevisiae. Microbiology. 1994, 140 (Pt 3): 601-610.View ArticleGoogle Scholar
- Tadege M, Kuhlemeier C: Aerobic fermentation during tobacco pollen development. Plant Mol Biol. 1997, 35 (3): 343-354. 10.1023/A:1005837112653.View ArticleGoogle Scholar
- op den Camp RG, Kuhlemeier C: Aldehyde dehydrogenase in tobacco pollen. Plant Mol Biol. 1997, 35 (3): 355-365. 10.1023/A:1005889129491.View ArticleGoogle Scholar
- Mellema S, Eichenberger W, Rawyler A, Suter M, Tadege M, Kuhlemeier C: The ethanolic fermentation pathway supports respiration and lipid biosynthesis in tobacco pollen. Plant J. 2002, 30 (3): 329-336. 10.1046/j.1365-313X.2002.01293.x.View ArticleGoogle Scholar
- Kirch HH, Bartels D, Wei Y, Schnable PS, Wood AJ: The ALDH gene superfamily of Arabidopsis. Trends Plant Sci. 2004, 9 (8): 371-377. 10.1016/j.tplants.2004.06.004.View ArticleGoogle Scholar
- Nair RB, Bastress KL, Ruegger MO, Denault JW, Chapple C: The Arabidopsis thaliana REDUCED EPIDERMAL FLUORESCENCE1 gene encodes an aldehyde dehydrogenase involved in ferulic acid and sinapic acid biosynthesis. Plant Cell. 2004, 16 (2): 544-554. 10.1105/tpc.017509.PubMed CentralView ArticleGoogle Scholar
- Millar AH, Sweetlove LJ, Giege P, Leaver CJ: Analysis of the Arabidopsis mitochondrial proteome. Plant Physiol. 2001, 127 (4): 1711-1727. 10.1104/pp.010387.PubMed CentralView ArticleGoogle Scholar
- Heazlewood JL, Tonti-Filippini JS, Gout AM, Day DA, Whelan J, Millar AH: Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins. Plant Cell. 2004, 16 (1): 241-256. 10.1105/tpc.016055.PubMed CentralView ArticleGoogle Scholar
- Skibbe DS, Liu F, Wen TJ, Yandeau MD, Cui X, Cao J, Simmons CR, Schnable PS: Characterization of the aldehyde dehydrogenase gene families of Zea mays and Arabidopsis. Plant Mol Biol. 2002, 48 (5–6): 751-764. 10.1023/A:1014870429630.View ArticleGoogle Scholar
- Gass N, Glagotskaia T, Mellema S, Stuurman J, Barone M, Mandel T, Roessner-Tunali U, Kuhlemeier C: Pyruvate decarboxylase provides growing pollen tubes with a competitive advantage in petunia. Plant Cell. 2005, 17 (8): 2355-2368. 10.1105/tpc.105.033290.PubMed CentralView ArticleGoogle Scholar
- Lin M, Oliver DJ: The role of acetyl-coenzyme a synthetase in Arabidopsis. Plant Physiol. 2008, 147 (4): 1822-1829. 10.1104/pp.108.121269.PubMed CentralView ArticleGoogle Scholar
- Chomczynski P, Sacchi N: Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987, 162 (1): 156-159. 10.1016/0003-2697(87)90021-2.View ArticleGoogle Scholar
- Puissant C, Houdebine LM: An improvement of the single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Biotechniques. 1990, 8 (2): 148-149. 10.1038/nbt0290-148.Google Scholar
- Huang Z, Yeakley JM, Garcia EW, Holdridge JD, Fan JB, Whitham SA: Salicylic acid-dependent expression of host genes in compatible Arabidopsis-virus interactions. Plant Physiol. 2005, 137 (3): 1147-1159. 10.1104/pp.104.056028.PubMed CentralView ArticleGoogle Scholar
- Sunkar R, Bartels D, Kirch HH: Overexpression of a stress-inducible aldehyde dehydrogenase gene from Arabidopsis thaliana in transgenic plants improves stress tolerance. Plant J. 2003, 35 (4): 452-64. 10.1046/j.1365-313X.2003.01819.x.View ArticleGoogle Scholar
- Swanson-Wagner RA, Jia Y, DeCook R, Borsuk LA, Nettleton D, Schnable PS: All possible modes of gene action are observed in a global comparison of gene expression in a maize F1 hybrid and its inbred parents. Proc Natl Acad Sci U S A. 2006, 103 (18): 6805-10. 10.1073/pnas.0510430103.PubMed CentralView ArticleGoogle Scholar
- Rozen S, Skaletsky H: Primer3 on the WWW for general users and for biologist programmers. Methods Mol Biol. 2000, 132: 365-386.Google Scholar
- Livak KJ, Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2001, 25 (4): 402-408. 10.1006/meth.2001.1262.View ArticleGoogle Scholar
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