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Table 1 Gene-specific primers used along with T-DNA left border primera for genotyping

From: The roles of aldehyde dehydrogenases (ALDHs) in the PDH bypass of Arabidopsis

 

T-DNA location

Gene Specific Primers

Insertion featureb

  

Forward

Reverse

 

aldh2C4-1 (SALK_027911)

Exon 3

5'-GCACAACTACTCA

TTTTTTTCT-3'

5'-TTGCGGCTGCGGC

TTGCATTATCT-3'

F/R

aldh2C4-2 (SALK_024974)

Exon 5

5'-TTGATGCGGTTGA

CGGTGGAAAAT-3'

5'-AACTTCTCCACAA

CCTTATCGTAT-3'

F

aldh2B4-1 (CSJ2971)

Intron 7

5'-GTTGGTCCTGCTC

TTGCTTGTGGTAA-3'

5'-TCGTTCGCCCTCT

TTATCACCTCATC-3'

R

aldh2B4-2 (SALK_078568)

Intron 1

5'-ATTCAAAGTACGG

CAACACAAACCAAGAG-3'

5'-TTACCACAAGCAA

GAGCAGGACCAAC-3'

F/R

aldh2B7-1 (CSJ989)

Exon 7

5'-TTGAGACTTGGGA

TAATGGGAAACCT-3'

5'-AAGAAAACTGTG

ACGGTAATAATCGG-3'

F

  1. The T-DNA insertion sites of mutant alleles were determined by sequencing. However as a consequence of the imprecision of T-DNA integration, it is not possible to establish the exact insertion positions. Only exon/intron positions of the insertions are determined. a JL202 was used for lines from the University of Wisconsin and LBa1 for those from the SALK institute. b "F" and "R" indicate amplification of insertion alleles using forward and reverse primers, respectively, in combination with T-DNA left border primer. "F/R" indicate amplification with both primer combinations.
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BMC Biochemistry

ISSN: 1471-2091

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