Fig. 3

Fibrinogenolysis activity of Dacin analyzed by 12% SDS-PAGE and its fibrinolysis activity tested by fibrin plate assay. a, Effects of different concentrations of Dacin incubated at 37 °C for 30 min with 25 μg of bovine fibrinogen. Lane 1, control; lane 2, 0.4 μg; lane 3, 0.8 μg; lane 4, 1.2 μg; lane 5, 1.6 μg; lane 6, 2 μg; lane 7, 2.4 μg; lane 8, 2.4 μg + 1 mM EDTA. b, Effects of 1.2 μg of Dacin incubated at 37 °C with 25 μg of bovine fibrinogen for different times. Lane 1, control; lane 2, 5 min; lane 3, 30 min; lane 4, 1 h; lane 5, 3 h; lane 6, 6 h; lane 7, 12 h; lane 8, 24 h. c, Effects of the different inhibitors on Dacin’s fibrinogenolysis activity. Lane 1, 5 mM EDTA; lane 2, 5 mM EGTA; lane 3, 5 mM 2-ME; lane 4, 5 mM PMSF; lane 5, negative control. d, Fibrin plate assay. 1, crude venom (20 μg); 2, Dacin (15 μg); 3, Dacin (10 μg); 4, saline. Every sample were inoculated into the wells in fibrin plate and incubated for 24 h at 37 °C