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Table 1 Enzyme activity parameters using different substrates

From: Substrate specificity and function of acetylpolyamine amidohydrolases from Pseudomonas aeruginosa

Enzyme   Ac-Cad Ac-Put N1-Ac-Spermine N1-Ac-Spermidine Boc-K-(Ac)-AMC Boc-K-(Tfa)-AMC
PA0321 KM/μM 284 ± 39c 411 ± 34c e e 22 ± 10 11 ± 1
Vmax/(nmole mg−1 s−1) 7.6 ± 0.3c 14.2 ± 0.4c e e 0.017 ± 0.006 21 ± 2
V/(nmole mg−1 s−1) 2.9 ± 0.1d 4.9 ± 0.2d ≤0.001d ≤0.001d - -
PA1409 KM/μM 465 ± 53c 503 ± 60c e e 223 ± 12 84 ± 15
Vmax/(nmole mg−1 s−1) 24.5 ± 0.9c 39.3 ± 1.5c e e 1.3 ± 0.1 5.2 ± 0.6
V/(nmole mg−1 s−1) 11.1 ± 0.3d 10.2 ± 0.1d 7.4 ± 0.1d 7.2 ± 0.2d - -
PA3774 KM/μM NAc NAc e e 95 ± 7 21 ± 2
Vmax/(nmole mg−1 s−1) ≤0.01c ≤0.01c e e 3.4 ± 0.1 8.6 ± 0.3
V/(nmol mg−1 s−1) ≤0.001d ≤0.001d ≤0.001d ≤0.001d - -
HDAH KM/μM - - - - 26 ± 4 7.2 ± 1.1
Vmax/(nmole mg−1 s−1) 0.18a 0.13a - 0.06a 0.43 ± 0.02 0.23 ± 0.01
APAH M. ramosa KM/μM 50b 220b 290b 130b - -
Vmax/(nmole mg−1 s−1) 450b 450b 223b 275b - -
  1. NA cannot be determined due to a very low signal, a values were taken from [46], b values were taken from [32], assay was performed at 30 °C, c this study: diamine oxidase assay performed at 21 °C, d: this study: acetate release assay at 200 μM substrate concentration, where Vmax is not reached, e: not compatible with diamine oxidase assay. Mean values and standard errors are based on at least three replicates