Table 1 Enzyme activity parameters using different substrates

PA0321

K_M/μM

284 ± 39^c

411 ± 34^c

^e

^e

22 ± 10

11 ± 1

V_max/(nmole mg⁻¹ s⁻¹)

7.6 ± 0.3^c

14.2 ± 0.4^c

^e

^e

0.017 ± 0.006

21 ± 2

V/(nmole mg⁻¹ s⁻¹)

2.9 ± 0.1^d

4.9 ± 0.2^d

≤0.001^d

≤0.001^d

-

-

PA1409

K_M/μM

465 ± 53^c

503 ± 60^c

^e

^e

223 ± 12

84 ± 15

V_max/(nmole mg⁻¹ s⁻¹)

24.5 ± 0.9^c

39.3 ± 1.5^c

^e

^e

1.3 ± 0.1

5.2 ± 0.6

V/(nmole mg⁻¹ s⁻¹)

11.1 ± 0.3^d

10.2 ± 0.1^d

7.4 ± 0.1^d

7.2 ± 0.2^d

-

-

PA3774

K_M/μM

NA^c

NA^c

^e

^e

95 ± 7

21 ± 2

V_max/(nmole mg⁻¹ s⁻¹)

≤0.01^c

≤0.01^c

^e

^e

3.4 ± 0.1

8.6 ± 0.3

V/(nmol mg⁻¹ s⁻¹)

≤0.001^d

≤0.001^d

≤0.001^d

≤0.001^d

-

-

HDAH

K_M/μM

-

-

-

-

26 ± 4

7.2 ± 1.1

V_max/(nmole mg⁻¹ s⁻¹)

0.18^a

0.13^a

-

0.06^a

0.43 ± 0.02

0.23 ± 0.01

APAH M. ramosa

K_M/μM

50^b

220^b

290^b

130^b

-

-

V_max/(nmole mg⁻¹ s⁻¹)

450^b

450^b

223^b

275^b

-

-