Figure 2From: Molecular evolution of B6 enzymes: Binding of pyridoxal-5'-phosphate and Lys41Arg substitution turn ribonuclease A into a model B6 protoenzymeFormation of internal aldimine between PLP and RNase. (---) Absorption spectrum of 30 μM PLP in 50 mM 4-methylmorpholine pH 7.5 at 25°C; (—) spectrum upon addition of 30 μM of RNase.Back to article page