Metabolic signature of breast cancer cell line MCF-7: profiling of modified nucleosides via LC-IT MS coupling

Bullinger, Dino; Neubauer, Hans; Fehm, Tanja; Laufer, Stefan; Gleiter, Christoph H; Kammerer, Bernd

doi:10.1186/1471-2091-8-25

BMC Biochemistry

Table 2 Metabolite excretion in cell culture supernatants of MCF-10A cells and MCF-7 breast cancer cells.

From: Metabolic signature of breast cancer cell line MCF-7: profiling of modified nucleosides via LC-IT MS coupling

No.	compound name	M+H⁺	MCF-10A	MCF-7
			AreaQ *1000^† mean (n = 3) ± SD	AreaQ *1000 mean (n = 3) ± SD
1	1-ribosylimidazole-4-acetic acid	259	19.83 ± 1.07	n.d.
2	Dihydrouridine	247	210.30 ± 19.13	261.58 ± 14.58
3	Pseudouridine	245	513.38 ± 26.77	658.02 ± 30.67
4	1-ribosylnicotinamide	255	1985.96 ± 206.67	729.10 ± 43.95
5	Cytidine	244	2044.16 ± 190.68	1066.82 ± 86.71
6	S-adenosylmethionine	399	n.d.	335.55 ± 21.75
7	5-carbamoylmethyluridine	302	32.47 ± 0.73	18.10 ± 0.96
8	3-(3-aminocarboxypropyl)-uridine	346	7.13 ± 0.63	12.89 ± 0.60
9	Uridine	245	1103.56 ± 30.38	504.13 ± 80.28
10	3-methylcytidine	258	382.01 ± 9.65	458.36 ± 16.08
11	1-ribosyl-4-carboxamido-5-aminoimidazole	259	n.d.	251.47 ± 22.35
12	1-methyladenosine	282	2170.75 ± 104.62	2067.45 ± 67.84
13	5-methylcytidine	258	437.16 ± 25.90	446.30 ± 14.00
14	Inosine	269	147.28 ± 9.77	46.89 ± 10.41
15	5-methyluridine	259	35.90 ± 1.48	141.70 ± 4.01
16	Guanosine	284	79.84 ± 8.73	15.31 ± 3.02
17	1-ribosyl-pyridin-2-one-5-carboxamide	271	56.25 ± 27.64	46.70 ± 4.94
18	3-methyluridine	259	20.30 ± 1.25	26.91 ± 0.59
19	Xanthosine	285	13.10 ± 0.27	18.96 ± 1.84
20	S-adenosylhomocysteine	385	7.53 ± 0.43	47.77 ± 0.98
21	1-methylinosine	283	282.12 ± 32.66	269.92 ± 6.25
22	1-methylguanosine	298	648.74 ± 5.15	700.59 ± 10.64
23	5'-deoxy-5'-methyl-thioadenosine sulfoxide	314	23.73 ± 0.95	32.40 ± 6.31
24	N⁴-acetylcytidine	286	296.08 ± 4.84	298.65 ± 6.69
25	N²-methylguanosine	298	293.26 ± 14.32	206.55 ± 7.32
26	Adenosine	268	5.97 ± 0.10	32.57 ± 7.16
27	N⁶-succinyloadenosine	384	130.04 ± 2.59	97.78 ± 3.40
28	N²,N²-dimethylguanosine	312	460.45 ± 19.86	587.62 ± 9.99
29	N²,N²,7-trimethylguanosine	326	129.55 ± 7.07	263.28 ± 5.54
30	5'-deoxyadenosine	252	101.50 ± 16.49	81.81 ± 2.47
31	5-methoxycarbonylmethyl-2-thiouridine	333	10.55 ± 0.23	12.60 ± 0.34
32	N⁶-methyladenosine	282	22.97 ± 11.04	32.67 ± 12.12
33	N⁶-threonylcarbamoyladenosine	413	42.34 ± 1.83	67.25 ± 0.44
34	5'-deoxy-5'-methyl-thioadenosine	298	3452.12 ± 213.17	5668.00 ± 125.15
35	N⁶-methyl-N⁶-threonylcarbamoyladenosine	427	3.11 ± 0.04	5.96 ± 0.46
36	2-methylthio-N⁶-threonylcarbamoyladenosine	459	3.68 ± 0.15	3.47 ± 0.16

^† AreaQ* 1000 = (peak area [M+H⁺ + M+Na⁺ + M+K⁺] (analyt)/peak area [M+H⁺ + M+Na⁺ + M+K⁺] (internal standard isoguanosine)) * 1000 (factor), tabulated by increasing retention time, n.d.: not detectable

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ISSN: 1471-2091

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