Table 1 Comparison of the spectral properties of zFP506, amFP486, and their mutants at position 167.
From: Color transitions in coral's fluorescent proteins by site-directed mutagenesis
Protein | Substitution | Excitation maximum, nm | Excitation spectral width, nm | Emission maximum, nm | Emission spectral width, nm |
|---|---|---|---|---|---|
zFP506 | Wild type | 494 | 41 | 506 | 23 |
| Â | M167A | 469 | 50 | 496 | 36 |
| Â | M167H | 469 | 53 | 497 | 36 |
amFP486 | Wild type | 453 | 58 | 486 | 49 |
| Â | A167M | 473 | 47 | 495 | 34 |
| Â | A167I | 464 | 56 | 498 | 38 |