Figure 1

SKIP augments AR-AF-1 dependent transactivation. Mammalian one-hybrid analysis in HEK293 cells that were transiently co-transfected with the GAL4₅E1bTATA-luciferase reporter (250 ng/well), with AR 1–555 N-terminal domain in pM-GAL4DBD expression vector (125 ng) or empty pM-GAL4DBD vector (125 ng), as well as with SKIP-pCGN (100 ng) or empty pCGN (100 ng). To allow for comparison to the N/C-interaction transfection experiments (Figure 2) the empty vector pVP16AD containing the VP16 transactivation domain was also transfected (125 ng). Data are relative to the control sample (no SKIP, no AR-AF1) as means ± SEM calculated from four independent luciferase reporter experiments, as described in Materials and Methods. Statistically significant difference between indicated means was determined using Student’s t-test.