Table 1 Effect of the concentration of ATP and C8D-ATP on the fit of the enzyme kinetic model of hexokinase, acetate kinase, adenylylated GS, deadenylylated GS, phosphofructokinase and shikimate kinase.

Shikimate kinase

Michaelis-Menton

Michaelis-Menton

1

v _max

2339 ± 600

2433 ± 600

K _m or K '

0.0065 ± 0.003

0.0128 ± 0.007

RMSD ^a

0.9543

0.9866

Hexokinase

Allosteric sigmoidal

Allosteric sigmoidal

1

v _max

1226 ± 189

1893 ± 940.7

K _m or K '

3.263 ± 0.662

4.419 ± 2.749

h

1.759 ± 0.207

1.020

RMSD ^a

0.9959

0.9919

Acetate kinase

Allosteric sigmoidal

Michaelis-Menton

1

v _max

22.29 ± 3.046

19.13 ± 2.103

K _m or K '

1.323 ± 0.353

1.081 ± 0.289

h

1.658 ± 0.302

RMSD ^a

0.9880

0.9704

Deadenylylated GS

Allosteric sigmoidal

Allosteric sigmoidal

1

v _max

11.49 ± 1.31

13.20 ± 0.651

K _m or K '

30.88 ± 4.44

6.774 ± 2.331

h

3.108 ± 0.316

4.077 ± 0.787

RMSD ^a

0.9972

0.9827

PFK ^b

Allosteric sigmoidal

Allosteric sigmoidal

2

v _max

111.3 ± 10.16

45.18 ± 1.012

K _m or K '

2.265 ± 0.320

0.097 ± 0.031

h

1.371 ± 0.098

1.785 ± 0.208

RMSD ^a

0.9982

0.9879

Adenylylated GS

Allosteric sigmoidal

Allosteric sigmoidal

>2

v _max

8.241 ± 0.787

6.540 ± 0.271

K _m or K '

15.22 ± 8.776

0.603 ± 0.151

h

3.288 ±

3.258 ± 0.589

RMSD ^a

0.9822

0.9924