Figure 2From: Biochemical characterization of malate synthase G of P. aeruginosaRecombinant production and purification of the malate synthase G of P. aeruginosa PAO1. (A) purification of malate synthase G after removal of the GST tag is shown and (B) after anion exchange chromatography (AEC). Samples from purified Gst-malate synthase fusion protein (Gst-PA0482), samples from the uninduced (UI) and induced (I) cultures are compared to the elution fractions (E1 to 5) after glutathione purification with removal of the GST tag and anion exchange chromatography. The amounts are not proportional between A and B. The low molecular weight ladder (LMW) served as reference for both gels (the molecular weight of the markers is indicated in kDa).Back to article page