Figure 3
From: Identification of sites phosphorylated by the vaccinia virus B1R kinase in viral protein H5R
Phosphorylation of synthetic peptides. Peptides were phosphorylated as described in the Experimental section and subjected to electrophoresis on thin-layer cellulose at pH 3.5. The position of the phosphopeptides is indicated by the arrow, the identification being based on the fact that the ninhydrin-stained material (predominantly unphosphorylated and hence with a greater positive charge) migrated just ahead of this. Lane 1 contained RRIEEYHQTTEKN, lane 2: RRIEEYHQA TEKN, lane 3: RRIEEYHQTA EKN, and lane 4; no peptide.