Nucleic acids biochemistry

Association of TM6SF2 rs58542926 gene polymorphism with the risk of non-alcoholic fatty liver disease and colorectal adenoma in Chinese Han population

Genetic factors affect the risk of non-alcoholic fatty liver disease (NAFLD) and colorectal adenoma (CRA) importantly. Transmembrane protein 6 superfamily member 2 (TM6SF2) rs58542926 is a significant genetic ...

Authors: Yuan Li, Shousheng Liu, Yuqiang Gao, Huan Ma, Shuhui Zhan, Yan Yang, Yongning Xin and Shiying Xuan

Identification of the major diacylglycerol acyltransferase mRNA in mouse adipocytes and macrophages

Triacylglycerols (TAGs) are the major form of energy storage in eukaryotes. Diacylglycerol acyltransferases (DGATs) catalyze the final and rate-limiting step of TAG biosynthesis. Mammalian DGATs are classified...

Authors: Heping Cao

Establishment of a detection assay for DNA endonuclease activity and its application in the screening and prognosis of malignant lymphoma

Endonucleases play critical roles in maintaining genomic stability and regulating cell growth. The purpose of this study was to evaluate detection of endonuclease activity as an indicator in the early diagnosi...

Authors: Renquan Lu, Yingchao Wang, Xiaofeng Xu, Suhong Xie, Yanchun Wang, Ailing Zhong, Hui Zheng, Yiwen Yu, Xiang Gao and Lin Guo

Knockdown of GGCT inhibits cell proliferation and induces late apoptosis in human gastric cancer

Gamma glutamylcyclotransferase (GGCT) has been proved to be involved in various cancers, but the biological function of GGCT in gastric cancer is still largely unknown.

Authors: Wenjie Zhang, Lei Chen, Honggang Xiang, Chunhua Hu, Weibin Shi, Ping Dong and Wenjie Lv

Altered activity patterns of transcription factors induced by endoplasmic reticulum stress

The endoplasmic-reticulum (ER) responds to the burden of unfolded proteins in its lumen by activating intracellular signal transduction pathways, also known as the unfolded protein response (UPR). Many signal ...

Authors: Sheena Jiang, Eric Zhang, Rachel Zhang and Xianqiang Li

Conserved motif VIII of murine DNA methyltransferase Dnmt3a is essential for methylation activity

Dnmt3a is a DNA methyltransferase that establishes de novo DNA methylation in mammals. The structure of the Dnmt3a C-terminal domain is similar to the bacterial M. HhaI enzyme, a well-studied prokaryotic DNA meth...

Authors: Olga V. Lukashevich, Natalia A. Cherepanova, Renata Z. Jurkovska, Albert Jeltsch and Elizaveta S. Gromova

Enzyme assays for synthesis and degradation of 2-5As and other 2′-5′ oligonucleotides

The 5′-triphosphorylated, 2′-5′-linked oligoadenylate polyribonucleotides (2-5As) are central to the interferon-induced antiviral 2-5A system. The 2-5As bind and activate the RNase L, an endoRNase degrading vi...

Authors: Jesper Buchhave Poulsen, Karina Hansen Kjær, Just Justesen and Pia Møller Martensen

The promoter of cell growth- and RNA protection-associated SND1 gene is activated by endoplasmic reticulum stress in human hepatoma cells

Staphyloccocal nuclease domain-containing protein 1 (SND1) is involved in the regulation of gene expression and RNA protection. While numerous studies have established that SND1 protein expression is modulated...

Authors: Sandra Armengol, Enara Arretxe, Leire Enzunza, Sarai Mula, Begoña Ochoa, Yolanda Chico and María José Martínez

Plasmodium falciparum UvrD activities are downregulated by DNA-interacting compounds and its dsRNA inhibits malaria parasite growth

Human malaria parasite infection and its control is a global challenge which is responsible for ~0.65 million deaths every year globally. The emergence of drug resistant malaria parasite is another challenge t...

Authors: Mohammed Tarique, Farha Tabassum, Moaz Ahmad and Renu Tuteja

AGG/CCT interruptions affect nucleosome formation and positioning of healthy-length CGG/CCG triplet repeats

Fragile X Syndrome (FXS), the most common inherited form of mental retardation, is caused by expansion of a CGG/CCG repeat tract in the 5′-untranslated region of the fragile X mental retardation (FMR1) gene, whic...

Authors: Catherine B Volle and Sarah Delaney

Power to the people: Does Eterna signal the arrival of a new wave of crowd-sourced projects?

Authors: Thomas A Rowles

Transcription start sites and epigenetic analysis of the HSD17B10 proximal promoter

Hydroxysteroid (17beta) dehydrogenase X (HSD10) is a multifunctional protein encoded by the HSD17B10 gene at Xp11.2. In response to stress or hypoxia-ischemia its levels increase rapidly. Expression of this gene ...

Authors: Song-Yu Yang, Carl Dobkin, Xue-Ying He and W Ted Brown

Hepatoma-derived growth factor and nucleolin exist in the same ribonucleoprotein complex

Hepatoma-derived growth factor (HDGF) is a protein which is highly expressed in a variety of tumours. HDGF has mitogenic, angiogenic, neurotrophic and antiapoptotic activity but the molecular mechanisms by whi...

Authors: Stephanie Bremer, Katharina Klein, Angela Sedlmaier, Mekky Abouzied, Volkmar Gieselmann and Sebastian Franken

Insertion of a myc-tag within α-dystroglycan domains improves its biochemical and microscopic detection

Epitope tags and fluorescent fusion proteins have become indispensable molecular tools for studies in the fields of biochemistry and cell biology. The knowledge collected on the subdomain organization of the t...

Authors: Simona Morlacchi, Francesca Sciandra, Maria Giulia Bigotti, Manuela Bozzi, Wolfgang Hübner, Antonio Galtieri, Bruno Giardina and Andrea Brancaccio

Bifunctional TaqII restriction endonuclease: redefining the prototype DNA recognition site and establishing the Fidelity Index for partial cleaving

The TaqII enzyme is a member of the Thermus sp. enzyme family that we propounded previously within Type IIS restriction endonucleases, containing related thermophilic bifunctional endonucleases-methyltransferases...

Authors: Agnieszka Żylicz-Stachula, Olga Żołnierkiewicz, Katarzyna Śliwińska, Joanna Jeżewska-Frąckowiak and Piotr M Skowron