Chemical biology

  1. Research article

    Antiproliferative factor (APF) binds specifically to sites within the cytoskeleton-associated protein 4 (CKAP4) extracellular domain

    Antiproliferative factor (APF) is a sialoglycopeptide elevated in the urine of patients with interstitial cystitis—a chronic, painful bladder disease. APF inhibits the proliferation of normal bladder epithelia...

    Burzin Chavda, Jun Ling, Thomas Majernick and Sonia Lobo Planey

    BMC Biochemistry 2017 18:13

    Published on: 11 September 2017

  2. Research article

    Serendipitous discovery of light-induced (In Situ) formation of an Azo-bridged dimeric sulfonated naphthol as a potent PTP1B inhibitor

    Protein tyrosine phosphatases (PTPs) like dual specificity phosphatase 5 (DUSP5) and protein tyrosine phosphatase 1B (PTP1B) are drug targets for diseases that include cancer, diabetes, and vascular disorders ...

    Robert D. Bongard, Michael Lepley, Khushabu Thakur, Marat R. Talipov, Jaladhi Nayak, Rachel A. Jones Lipinski, Chris Bohl, Noreena Sweeney, Ramani Ramchandran, Rajendra Rathore and Daniel S. Sem

    BMC Biochemistry 2017 18:10

    Published on: 31 May 2017

  3. Research article

    Avoiding false discovery in biomarker research

    Human tyrosine-protein phosphatase non-receptor type substrate 1α (SIRPA) is a surface marker identified in cardiomyocytes differentiated from human embryonic stem cells. Our objective was to determine if circ...

    Pranali Patel, Uros Kuzmanov and Seema Mital

    BMC Biochemistry 2016 17:17

    Published on: 30 July 2016

  4. Research article

    Tubulin is a molecular target of the Wnt-activating chemical probe

    In drug discovery research, cell-based phenotypic screening is an essential method for obtaining potential drug candidates. Revealing the mechanism of action is a key step on the path to drug discovery. Howeve...

    Yasunori Fukuda, Osamu Sano, Kenichi Kazetani, Koji Yamamoto, Hidehisa Iwata and Junji Matsui

    BMC Biochemistry 2016 17:9

    Published on: 20 May 2016

  5. Research article

    Comparative sequence analysis and mutagenesis of Ethylene Forming Enzyme (EFE) 2-oxoglutarate/Fe(II)-dependent dioxygenase homologs

    Ethylene is one of the most used chemical monomers derived from non-renewable sources and we are investigating the possibility of producing it in yeast via the ethylene forming enzyme (EFE) from Pseudomonas syrin...

    Nina Johansson, Karl O Persson, Christer Larsson and Joakim Norbeck

    BMC Biochemistry 2014 15:22

    Published on: 2 October 2014

  6. Research article

    Chemical-genetic induction of Malonyl-CoA decarboxylase in skeletal muscle

    Defects in skeletal muscle fatty acid oxidation have been implicated in the etiology of insulin resistance. Malonyl-CoA decarboxylase (MCD) has been a target of investigation because it reduces the concentrati...

    Susana Rodriguez, Jessica M Ellis and Michael J Wolfgang

    BMC Biochemistry 2014 15:20

    Published on: 25 August 2014

  7. Research article

    Comparative analysis of diosgenin in Dioscorea species and related medicinal plants by UPLC-DAD-MS

    Dioscorea is a genus of flowering plants, and some Dioscorea species are known and used as a source for the steroidal sapogenin diosgenin. To screen potential resource from Dioscorea species and related medicinal...

    Tao Yi, Lan-Lan Fan, Hong-Li Chen, Guo-Yuan Zhu, Hau-Man Suen, Yi-Na Tang, Lin Zhu, Chu Chu, Zhong-Zhen Zhao and Hu-Biao Chen

    BMC Biochemistry 2014 15:19

    Published on: 9 August 2014

  8. Research article

    Galloyl-RGD as a new cosmetic ingredient

    The cosmetics market has rapidly increased over the last years. For example, in 2011 it reached 242.8 billion US dollars, which was a 3.9% increase compared to 2010. There have been many recent trials aimed at...

    Dae-Hun Park, Dae Hyun Jung, Soo Jung Kim, Sung Han Kim and Kyung Mok Park

    BMC Biochemistry 2014 15:18

    Published on: 8 August 2014

  9. Methodology article

    Determination of carbohydrate-deficient transferrin in a Han Chinese population

    Carbohydrate-deficient transferrin (CDT) is a widely used alcohol biomarker. Because of the high prevalence of chronic alcohol abuse in many countries, CDT plays an important role in the areas of traffic, clin...

    Binbin Song, Jing Zhu, Jiong Wu, Chunyan Zhang, Beili Wang, Baishen Pan and Wei Guo

    BMC Biochemistry 2014 15:5

    Published on: 27 February 2014

  10. Research article

    Effect of the expression and knockdown of citrate synthase gene on carbon flux during triacylglycerol biosynthesis by green algae Chlamydomonas reinhardtii

    The regulation of lipid biosynthesis is essential in photosynthetic eukaryotic cells. This regulation occurs during the direct synthesis of fatty acids and triacylglycerols (TAGs), as well as during other cont...

    Xiaodong Deng, Jiajia Cai and Xiaowen Fei

    BMC Biochemistry 2013 14:38

    Published on: 30 December 2013

  11. Methodology article

    Development of an ELISA assay for the quantification of soluble huntingtin in human blood cells

    Huntington’s disease (HD) is a monogenic disorder caused by an aberrant expansion of CAG repeats in the huntingtin gene (HTT). Pathogenesis is associated with expression of the mutant (mHTT) protein in the CNS, w...

    Luisa Massai, Lara Petricca, Letizia Magnoni, Luca Rovetini, Salman Haider, Ralph Andre, Sarah J Tabrizi, Sigurd D Süssmuth, Bernhard G Landwehrmeyer, Andrea Caricasole, Giuseppe Pollio and Simonetta Bernocco

    BMC Biochemistry 2013 14:34

    Published on: 25 November 2013

  12. Research article

    Cell attachment on poly(3-hydroxybutyrate)-poly(ethylene glycol) copolymer produced by Azotobacter chroococcum 7B

    The improvement of biomedical properties, e.g. biocompatibility, of poly(3-hydroxyalkanoates) (PHAs) by copolymerization is a promising trend in bioengineering. We used strain Azotobacter chroococcum 7B, an effec...

    Anton P Bonartsev, Sergey G Yakovlev, Irina I Zharkova, Arasha P Boskhomdzhiev, Dmitrii V Bagrov, Vera L Myshkina, Tatiana K Makhina, Elena P Kharitonova, Olga V Samsonova, Alexey V Feofanov, Vera V Voinova, Anton L Zernov, Yurii M Efremov, Garina A Bonartseva, Konstantin V Shaitan and Michail P Kirpichnikov

    BMC Biochemistry 2013 14:12

    Published on: 21 May 2013