Table 1 Purification scheme for meswak protease
From: Purification and characterization of cysteine protease from miswak Salvadora persica
Steps | T. units | T. Protein mg | S.Aa | Fold purification | Recovery 100% |
|---|---|---|---|---|---|
Crude extract | 545 | 14 | 39 | 1 | 100 |
Chromatography on DEAE-Sepharose | |||||
0.0 M NaCl (P1) | 412 | 4.6 | 89.5 | 2.29 | 75 |
0.1 M NaCl (P2) | 40 | 2.2 | 18 | 0.46 | 7.3 |
0.2 M NaCl (P3) | 60 | 6.5 | 9.2 | 0.23 | 11 |
Sephacryl S-200 P1 | 355 | 1.0 | 355 | 9.1 | 65 |