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Table 3 Kinetic parameters for the four reactions systems studied

From: Feruloyl esterase immobilization in mesoporous silica particles and characterization in hydrolysis and transesterification

 

Kma (mM)

kcata (s− 1)

kcat/Kma (s− 1 M− 1)

Free enzyme - hydrolysis

0.43 ± 0.07

31.4 ± 2.20

7.29.104 ± 1.7.104

Free enzyme - transesterification

36.0 ± 14.4

0.14 ± 0.03

3.79 ± 2.33

Immobilized enzyme - hydrolysis

0.43 ± 0.06

6.47 ± 0.34

1.5.104 ± 0.3.104

Immobilized enzyme - transesterification

30.9 ± 7.30

0.01 ± 1.10− 3

0.35 ± 0.11

  1. aData are presented as mean ± standard deviation
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BMC Biochemistry

ISSN: 1471-2091

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