Fig. 5

Determination of pH and temperature optima for the four reaction systems studied a Optimum pH. Reaction rates measured using phosphate-citrate buffer at pH 5.0–8.0 and 40 °C. b Optimum temperature. The temperature was varied from 15 to 80 °C and the reaction was performed using phosphate-citrate buffer at pH 7.0. All reactions were in initial velocity. The results are the average of triplicate samples, and the error bars represent one standard deviation