Fig. 5
From: Characterization of sulfhydryl oxidase from Aspergillus tubingensis
Oxidation of GSH by AtSOX as analysed by two-dimensional heteronuclear NMR spectroscopy (13C-HSQC spectra showing one-bond 1H-13C connectivities). The reduced substrate is shown on the left side as control and the enzyme-treated sample on the right side. The assignment of the tripeptide is indicated on the top of the cross-peaks. Arrows emphasize the chemical shift change in the Cβ correlation of cysteine in the two conformations i.e. in the cross-linked and open form (a). The gradient selected 13C-HSQC spectrum of the substrate control to evaluate auto-oxidation three days after the preparation (b)