Fig. 9

4 μM CaATP-G-actin was digested by 1.5 μg/ml subtilisin or 20 μg/ml trypsin at 22 °C for 10 min and was cross-linked to 6 μM Fl-histatin-3 and Fl-histatin-5 by 0.3 mg/ml TGase at 22 °C for 30 min. Trypsin and subtilisin digestions were stopped by 40 μg/ml STI and 1 mM PMSF, respectively. Samples were run on SDS-PAGE. Left, lanes visualized by Coomassie blue; right, lanes visualized by fluorescence, marked by (*). Legend: (a) actin; (b) trypsin digested actin; (c) trypsin digested actin cross-linked with Fl-histatin-3 or Fl-histatin-5; (d) subtilisin digested actin; (e) subtilisin digested actin cross-linked with Fl-histatin-3 or Fl-histatin-5; nf), actin cross-linked with Fl-histatin-3 or Fl-histatin-5. a Fl-histatin-3-actin fragments cross-linked bands; b Fl-histatin-5-actin fragments cross-linked bands. Lane M, molecular weight marker