Effect of mutations to amino acid A301 and F361 in thermostability and catalytic activity of the β-galactosidase from Bacillus subtilis VTCC-DVN-12-01

Table 1 Mutations of selected epRCA transformants

Transformant	RA (%)	Nucleotide substitution	Amino acid substitution
Wild-type	100	original
LacA125	167	No
LacA221	150	No
LacA887	198	ACG → ACA, CGG → CGA	T408, R624
LacA259	52	UUC → UAU, GCU → ACU	F361Y, A524T
LacA461	36	GAG → GAA, CGG → UGG, GCG → GUG, GCG → GUG	E60, R77W, A191V, A301V

Plasmids from selected colonies were isolated and sequenced to identify mutations in the lacA gene

ISSN: 1471-2091