Tubulin is a molecular target of the Wnt-activating chemical probe

BMC Biochemistry

Table 3 Assay conditions such as cell lines, cell densities, and incubation times for cellular proliferation assays in pathway profiling

Cell lines	Cell density (cells/well)	Incubation time with compounds
HEK293T	5,000	20 h
Jurkat	5,000	20 h
MRC5	1,000	72 h
MRC5	3,500	72 h
A549	1,000	72 h
PC3	1,000	72 h
LNCaP	600	72 h
MDA-MB-231	500	72 h

ISSN: 1471-2091