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Table 3 Assay conditions such as cell lines, cell densities, and incubation times for cellular proliferation assays in pathway profiling

From: Tubulin is a molecular target of the Wnt-activating chemical probe

Cell lines Cell density (cells/well) Incubation time with compounds
HEK293T 5,000 20 h
Jurkat 5,000 20 h
MRC5 1,000 72 h
MRC5 3,500 72 h
A549 1,000 72 h
PC3 1,000 72 h
LNCaP 600 72 h
MDA-MB-231 500 72 h