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Fig. 2 | BMC Biochemistry

Fig. 2

From: The eukaryotic translation initiation factor 3f (eIF3f) interacts physically with the alpha 1B-adrenergic receptor and stimulates adrenoceptor activity

Fig. 2

The 120 kDa complex is composed by eIF3f and the alpha-1B adrenergic receptor. a Resolved proteins in a SDS-PAGE from anti-eIF3f immunoprecipitate of the 120 kDa region: the expected 47 kDa corresponding to eIF3f, and an unknown ~60 kDa protein. The ~60 kDa protein was sent for N-terminal sequencing and a Blastp showed that it could be the alpha 1B-ADR. MW, prestained protein ladder; A549, resolved proteins in the 120 kDa complex obtained from total native protein extracts. b The 120 kDa complex was immunoprecipitated in native conditions with anti-eIF3f, and immunodetected with anti-alpha 1B-ADR or anti-eIF3f in denaturing conditions. c The 120 kDa complex was immunoprecipitated in native conditions with anti-alpha 1B-ADR, and immunodetected with anti-eIF3f or anti-alpha 1B-ADR in denaturing conditions. d Immunodetection of alpha 1B-ADR in native conditions using A549 cell extracts (MW: native protein standard), where the 120 kDa band is present. Results show that alpha 1B-ADR is the eIF3f partner in the 120 kDa complex

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BMC Biochemistry

ISSN: 1471-2091

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