Table 1 Positive clones identified in substrate-trap screening of a human brain cDNA library using LexA-laforin C266S as bait. Yeast THY-AP4 cells transformed with plasmid pBridge-laforin C266S were co-transformed with a human brain cDNA library in pACT2
From: The laforin/malin E3-ubiquitin ligase complex ubiquitinates pyruvate kinase M1/M2
No. of positive clones | Encoded protein | Uniprot number | Intensity of interaction |
|---|---|---|---|
5 | Pyruvate kinase PKM1/PKM2 | Q504U3 | From ++ to +++ |
3 | E3 ubiquitin ligase TOPORS | Q9NS56 | ++ |
3 | Cytochrome C oxidase subunit 5A | P20674 | ++ |
1 | Laforin (EPM2A) | O95278 | +++ |
1 | PP1 regulatory subunit R5/PTG (PPP1R3C) | Q9UQK1 | ++ |
1 | G/T mismatch-specific thymine DNA glycosylase | Q13569 | +++ |
1 | 26S proteasome regulatory subunit 6A | P17980 | ++ |
1 | HMG domain-containing protein 4 | Q9UGU5 | ++ |
1 | Translation initiation factor eIF-2B subunit alpha | Q14232 | ++ |