Fig. 5

a Gel filtration of SNTI on Sephadex G-150. Elution profile of SNTI on a calibrated column of Sephadex G-150. 10 mg of purified SNTI was applied to the column in phosphate buffer pH 7.6 containing 20 mM NaCl and eluted with same buffer. Fractions of 4 ml each were collected at flow rate of 12 ml per hour. Protein was monitored at 280 nm. b Molecular weight determination of SNTI by gel filtration on Sephadex G-150. Plot of elution volume against log molecular weight of standard proteins. BSA −66 K.Da Ovalbumin −43 K.Da. Carbonicanhydrase −29 K.Da Lysozyme −14.3 K.Da