Fig. 4

Complex II and III enzymatic activities are similar in mitochondrial preparations of brain and liver mitochondria but differs in response to Antimycin A. a Mitochondrial complex II enzymatic activity was assessed by following the reduction of DCIP by 1.5 μg of isolated brain or liver mitochondria over one minute. b Mitochondrial complex III enzymatic activity was assessed by following the reduction of cytochrome c by 20 μg by isolated brain or liver mitochondria over one minute. Basal indicates the total complex III activity in the absence of electron transport chain inhibitors. When indicated, antimycin A (10 μM), DPI (10 μM), or CMB (10 μM) was added before the initiation of the reaction. Symbols denote comparisons among brain mitochondria treatment groups or liver mitochondria treatment groups as well as comparisons between brain and liver mitochondria for each treatment. For each comparison, * vs. # < 0.01; * vs. † < 0.001; # vs. † < 0.01. For each condition, n = 3