Fig. 4
From: Design of symmetric TIM barrel proteins from first principles
Ab initio modeling of Octarellin V and Octarellin VI. Both sequences were divided into four quadrants each before ab initio modeling. a-d The lowest scoring modeled structures for all fragments of Octarellin V, arranged from N to C-terminal. The expected alpha/beta secondary structure topology is not observed. Instead, all fragments adopt mostly helical secondary structures. e-h The lowest scoring modeled structures for all fragments of Octarellin VI, arranged from N to C-terminal. Although some beta sheets are observed, the overall topology is inconsistent with that expected for a TIM barrel. Note that the intended design of secondary structure is depicted in colors. Designed alpha helices are colored teal. Designed beta sheets are colored orange. Designed loops are colored green