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Fig. 2 | BMC Biochemistry

Fig. 2

From: Structural plasticity of green fluorescent protein to amino acid deletions and fluorescence rescue by folding-enhancing mutations

Fig. 2

Sequence analysis of identified deletions in GFPUV. α-helices and β-strands are shown as cylinders and arrows, respectively. The chromophore is indicated by a star. Deletions leading to fluorescent and non-fluorescent phenotypes are shown as white and black triangles, respectively. Fluorescent mutants include S2/K3➔R, G4Δ, E5Δ, F8/T9➔S, C48Δ, P75/D76➔H, P75Δ, E172Δ, S175/V176➔F, A226Δ, G228Δ and G228/I229➔V (M1Δ mutant is not included due to the deletion of the start codon). Non-fluorescent mutants include G10Δ, D21Δ, G24Δ, E34Δ, P58Δ, P89/E90➔Q and T203Δ

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