Fig. 1
From: Enzyme assays for synthesis and degradation of 2-5As and other 2′-5′ oligonucleotides
Production of single 2-5As and 2-5A core molecules. 2-5As were synthesized using human recombinant His-OAS1 protein, and subsequently resolved and concentrated by Mono Q chromatography. (a-e) Chromatograms representing different stages of the procedure. (a) Analysis of 2-5A synthesis. (b and c) Large-scale fractionation of 2-5As and 2-5A core molecules. (d) Example of up-concentration of a 2′-5′ oligoadenylate. In this case the A(pA)3 tetramer core. (e) Quality check of a 2′-5′ oligoadenylate (again the A(pA)3). Blue curves: The 2′-5′ oligoadenylates numbered according to length, hence 2 specify the pppApA (or ApA) dimer, 3 the pppA(pA)2 (or A(pA)2) trimer, 4 the pppA(pA)3 (or A(pA)3) tetramer and so forth. Red curve in (a): Chromatogram obtained using control molecules: Adenosine, 5′-AMP, 5′-ADP and 5′-ATP as indicated. Brown curves: Experimental salt gradients. mAU (milli-absorbance unit) is the unit used for molecular detection at 254 nm, and mS/cm (milliSiemens/centimeter) the unit used to measure conductivities