Figure 4

Activation of self-renewal/proliferation related signaling pathways in LNCaP S/P cells. (A) The probably related signaling pathways in parental, S/P, and non S/P cells of LNCaP cell line were examined by Western blot analysis, such as AKT, Bcl-2, c-Myc, MAPK (Erk1/2) and Stat3. (B) Cells (LNCaP S/P) were infected with lentivirus carrying V/AR and the levels of AR, p-Akt, bcl-2, and c-Myc were examined by Western blot analysis. (C) The S/P cells of LNCaP cell line were transfected with AR, Oligo bcl-2 siRNA, or/and c-Myc siRNA plasmids indicated. At the end of 4 days of culture after transfection, cells were used to do MTT assay.