Figure 2

Identification of EMT characteristics in S/P cells from LNCaP cell line. (A) Western blot analysis of AR, E Cadherin, N Cadherin, Vimentin and Snail using cell extracts of parental, S/P and non S/P cells. (B) IF staining results using antibodies of E Cadherin, N Cadherin and Vimentin. (C) Soft agar colony formation assay. The plates were incubated at 37°C in a humidified incubator for 21 days. Colonies larger than 1 mm in diameter were counted. (D) Migration assay. The migrative potential of different cells was assessed using the 8 μm pore Transwell system. The average number of cells per field of view (five random fields per membrane) was counted at × 20 magnification.