Figure 1

Isolating S/P cells from LNCaP cell line. (A) Flow cytometry isolation of S/P (1.5%) and non S/P (98.5%) cells. Cells were grown in regular RPMI medium containing 5% of FBS (cells of passage number less than 3 were used for all experiments). (B) Morphology of sorted S/P cells. (C) Soft agar assay of S/P and non S/P cells. (D) Western blot analysis of AR, CD133, Integrin β1, and CK 8 using cell extracts of parental, S/P, and non S/P cells. (E) IF staining results using antibodies of CK5, CK8, AR, CD133 and Integrin. (F) qRT-PCR analysis results of markers of parental, S/P, and non S/P cells.