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Table 1 Metal contents and specific activities of selected batches of recombinant QueD proteins

From: Nickel quercetinase, a “promiscuous” metalloenzyme: metal incorporation and metal ligand substitution studies

Protein a

Metal content (equivalents per protein monomer)

Specific activity (U mg −1 )

Ni-QueD

0.70 Ni, 0.20 Fe, 0.03 Co, 0.06 Zn, 0.01 Mn

137

Co-QueD

0.51 Co, 0.07 Ni, 0.05 Fe, 0.02 Zn, 0.01 Mn, 0.01 Cu

30

Mn-QueD

0.44 Mn, 0.12 Zn, 0.08 Fe, 0.05 Ni, 0.01 Co, 0.01 Cu

18

Fe-QueD

0.72 Fe, 0.05 Ni, 0.02 Zn, 0.02 Cu, 0.01 Mn

8

  1. aThe designations of the QueD metal forms refer to the metal of interest and do not imply exclusive occupancy of the protein with this metal.
  2. Metal ions which contribute to ≥0.01 equivalents per protein monomer are listed.
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BMC Biochemistry

ISSN: 1471-2091

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