Figure 1From: Solution structure and biophysical characterization of the multifaceted signalling effector protein growth arrest specific-1 Purification of recombinant GAS1 from insect cells. A) Ni-affinity chromatogram for GAS1 purification B) Western blot of fractions from the Ni-affinity chromatography peak at ca. 28Â ml. Fractions of 1Â ml were collected, and fractions from peak area at 23, 27, 29 and 33Â ml were tested in the western blot. C) SDS-PAGE analysis of GAS1 purification (left lane, molecular weight marker, with sizes indicated; right lane, purified GAS1 after gel filtration; the gel was Coomassie stained.).Back to article page