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Figure 6 | BMC Biochemistry

Figure 6

From: The exchanged EF-hands in calmodulin and troponin C chimeras impair the Ca2+-induced hydrophobicity and alter the interaction with Orai1: a spectroscopic, thermodynamic and kinetic study

Figure 6

Kinetics of Orai-CMBD to CaM and chimeras. A solution of 5 μM dansyl-CaM, dansyl-CaM(1TnC), or dansyl-CaM(3TnC) and 15 μM Orai-CMBD was quickly mixed with a solution containing 75 μM CaM in the presence of 2 mM Ca2+ and the fluorescence decay was monitored for 2 s for CaM (A) and CaM(1TnC) (B) and 5 s for CaM(3TnC) (C) (left panel). The decay was fitted with a single exponential decay and the resulting k off values are shown in Table 4. The peptide dissociated from dansyl-CaM or chimeras from the mixing resulted in the intensity decrease as observed in the complexed and uncomplexed fluorescently labeled CaM or chimeras (right panel).

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BMC Biochemistry

ISSN: 1471-2091

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