SPR analysis of binding of leptin to the leptin receptor and its inhibition by the gluten digest.
A) Examples of sensorgrams recorded during the injection of 30 nM leptin alone (black) or in the presence of 0.0022 (blue), 0.0045 (green), 0.018 (orange) or 0.3 (red) μg/mL gluten digest over a Sensor Chip with immobilized leptin receptor-Fc chimera. The pink dashed line is a fit to the black line using equation 2 (see Methods). B) Example of a sensorgram recorded during buffer flow after complete injection of 30 nM leptin (black). The pink dashed line is a fit to the data using equation 1 (see Methods). The two vertical lines occur during the extended time dissociation when the machine switches between its two pumps. C) Plot of relative intensity at the final leptin binding plateau during injection of 30 nM leptin versus gluten digest concentration. The error bars represent the standard deviation of three measurements.