Folding and self-association of atTic20 in lipid membranes: implications for understanding protein transport across the inner envelope membrane of chloroplasts

Table 1 Secondary structure composition of atTic20 and its truncated mutant in detergents and lipid vesicles ^a

Proteins	Environment	α	β	Turn	Random	NRMSD
atTic20	CHAPS micelles	40	11	14	27	0.036
	LDAO micelles	7	30	19	43	0.035
	TX- 100 micelles	19	25	20	36	0.032
	ZW 3-14 micelles	47	12	18	23	0.028
	POPC vesicles	74	11	5	9	0.002
atTic20	Distill prediction*	69	--	--	--	------
atTic20ΔN20	ZW 3-14 micelles	43	20	16	21	0.013

^aDeconvolution of CD spectra was performed using the CDSSTR program on the Dichroweb website (See Methods). The values represent the percentage of secondary structure composition. NRMSD, normalized root mean square deviation, denotes the best fit between the calculated and experimental CD spectra.
*Helical content of atTic20 was predicted using the Distill prediction program, as described in the Methods.

ISSN: 1471-2091