Figure 2

Biotin signals on the self-modified wild type CTc is an ADP-ribosylation reaction. (A) Purified wild type CTc or inactive mutant CTc(YEDQ), was incubated with various concentrations of biotinyl-NAD⁺. The folds of fluorescence intensity increase between CTc and CTc(YEDQ) were shown on the top of each pair of bar graph. (B) Purified CTc or CTc(YEDQ) was incubated with various concentrations of [carbonyl-¹⁴C]-NAD⁺ at 37°C for 1 hour. Asterisk indicates the control group in which 293 cell lysate was added to CTc or CTc(YEDQ) to measure ¹⁴C incorporation into the TCA precipitants. Data were summarized from two separated experiments.