Figure 8
From: Prion protein insertional mutations increase aggregation propensity but not fiber stability
Expansion of the ORD enhances fiber formation in the presence of the denaturant urea. Recombinant protein was diluted from denaturant (120 ×) to 2.5 μM and fiber formation was followed by Th-T fluorescence. Fiber formation of chimeric proteins SP5NM, SP8NM, SP11NM and SP14NM in (A) 0.5 M Urea FFB (B) 1 M Urea FFB is shown. Data in the graphs are representative of two experiments.