Table 2 Biochemical and kinetic properties of inulosucrase (IslA) from L. citreum CW28 and truncated versions.
From: Functional role of the additional domains in inulosucrase (IslA) from Leuconostoc citreum CW28
Truncated version | Optimum T (°C) | Half life @ 35°C (min) | Hydrolysis/transglycosylase ratio (%)a | KmT(mM) | kcat (s-1) | kcat/Km (mM-1s-1) |
|---|---|---|---|---|---|---|
IslA | 35 | 420 | 40/60 | 38 | 25 | 0.65 |
IslA2 | 35 | 407 | 38/62 | 24 | 28.19 | 1.17 |
IslA3 | 30 | 346 | 61/39 | nd | nd | nd |
IslA4 | 30 | 128 | 70/30 | 142 | 105 | 0.73 |