Figure 5

Circular Dichroism spectra of native IslA and IslA4 mediated by EDTA and Ca²⁺ ions. (a) IslA4: control (yellow line), IslA4 after incubation for 15 min with 0.5 mM EDTA (brown line); IslA4 after incubation for 15 min of the previous sample after restoring Ca²⁺ ions (1 mM) (green line). (b) IslA: control (yellow line), IslA after incubation for 180 min with 0.5 mM EDTA (brown line); IslA after incubation for 180 min of the previous sample after restoring Ca²⁺ ions (8 mM) (green line).