Figure 1

Preparative size exclusion chromatography of recombinant MCR1. A representative elusion profile of MCR1 from a 120 ml Superdex 200 gel filtration column is shown, which is the final chromatographic separation in MCR1 purification from bacterial cells. The elution positions of three separate sample pools used in this study, referred to as Fractions A, B, and C, are indicated with brackets. Collection of Fraction C commenced at the trailing edge of the void volume peak (at 1/3 peak height), and continued to a slight peak trough, where collection of Fraction B was begun. Fraction A was collected from the second peak trough through the trailing edge of the dimeric MCR1 peak (at 1/3 peak height). Inset: Coomassie Blue-stained SDS polyacrylamide gel (10%) of Fractions A, B, and C samples after purification and concentration to similar levels. The first lane contains molecular weight markers with the sizes of selected markers labeled (in kDa). The predicted molecular weight of monomeric MCR1 is 115.7 kDa.