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Table 2 Kinetic characterization of the MurF enzyme velocities in respect to the D-Ala-D-Ala substrate

From: Phage display-derived inhibitor of the essential cell wall biosynthesis enzyme MurF

Kinetic parameters

MurF

Specific activity (μmol/min/mg)

4.4 ± 0.1

kcat (min-1)

100 ± 15

Km (μM)

115 ± 10

kcat/Km (min-1 μM-1)

0.85 ± 0.15